We narrowed to 11,282 results for: Vars;

1. pQE-WT-RGVG-M6

   Plasmid

   #70172

   Purpose

   Expresses RGVG-M6 T7 RNA polymerase variant with enhanced transcription of 2' modified RNA

   Depositor

   Andrew Ellington

   Article

   Meyer et al Nucleic Acids Res. 2015 Sep 3;43(15):748

   Insert

   RGVG-M6 T7 RNA polymerase
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T5/Lac

   Available Since

   Dec. 16, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pAS1NB m Rosella I

   Plasmid

   #71247

   Purpose

   a biosensor comprised of a fast-maturing pH-stable red fluorescent protein fused to a pH-sensitive green fluorescent protein variant. Mitochodrial localization.

   Depositor

   Mark Prescott

   Article

   Rosado et al Autophagy. 2008 Feb;4(2):205-13. Epub 20

   Insert

   Rosella
   

   Tags

   yeast mitochondrial targeting sequence of citrate…

   Expression

   Yeast

   Promoter

   PGK1

   Available Since

   Nov. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. IRF3-V5

   Plasmid

   #169775

   Purpose

   Express IRF3 in mammalian cells

   Depositor

   Sung-Liang Yu

   Article

   Chang et al Commun Biol. 2021 Jun 2;4(1):663. doi: 1

   Insert

   Homo sapiens interferon regulatory factor 3 (IRF3), transcript variant 1 (IRF3 Human)
   

   Tags

   V5 tag

   Expression

   Bacterial and Mammalian

   Available Since

   July 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.2-hBIN1-V5

   Plasmid

   #27303

   Depositor

   Robin Shaw

   Article

   Hong et al PLoS Biol. 2010 Feb . 8(2):e1000312.

   Insert

   bridging integrator 1 (BIN1), transcript variant 8 (BIN1 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Feb. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pDEST/N1-hBIN1-GFP

   Plasmid

   #27305

   Depositor

   Robin Shaw

   Article

   Hong et al PLoS Biol. 2010 Feb . 8(2):e1000312.

   Insert

   bridging integrator 1 (BIN1), transcript variant 8 (BIN1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Jan. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. TALEN-CCR5L18-CtermQ3

   Plasmid

   #51440

   Purpose

   Expresses TALEN targeting left CCR5A site (L18) with Q3 C-terminal domain

   Depositor

   David Liu

   Article

   Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

   Insert

   TALEN CCR5 Left (L18) Q3 C-term
   

   Use

   TALEN

   Tags

   3xFLAG and Fok1 EL variant

   Expression

   Mammalian

   Mutation

   K788Q, R792Q and R801Q

   Promoter

   CMV

   Available Since

   April 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. TALEN-CCR5R18-CtermQ3

   Plasmid

   #51441

   Purpose

   Expresses TALEN targeting right CCR5A site (R18) with Q3 C-terminal domain

   Depositor

   David Liu

   Article

   Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

   Insert

   TALEN CCR5 Right (R18) Q3 C-term
   

   Use

   TALEN

   Tags

   3xFLAG and Fok1 KK variant

   Expression

   Mammalian

   Mutation

   K788Q, R792Q and R801Q

   Promoter

   CMV

   Available Since

   April 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. P1K-3HA-Atg8∆R-MET15

   Plasmid

   #207034

   Purpose

   Expression of 3HA-Atg8∆R. This variant bypasses the need of Atg4 for initial C-terminal processing.

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   ATG8
   

   Tags

   3xHA

   Expression

   Yeast

   Mutation

   ∆R

   Promoter

   pATG8

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. Stat3 pcDNA3

   Plasmid

   #8706

   Purpose

   See Depositor Comments below for variant information.

   Depositor

   Jim Darnell

   Article

   Zhong et al Proc Natl Acad Sci U S A 1994 May 24;91(

   Insert

   Stat3 (Stat3 Mouse)
   

   Expression

   Mammalian

   Mutation

   * see comments

   Available Since

   Sept. 26, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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10. TALEN-CCR5L18-NtermN1

    Plasmid

    #51444

    Purpose

    Expresses TALEN targeting left CCR5A site (L18) with N1 N-terminal domain

    Depositor

    David Liu

    Article

    Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

    Insert

    TALEN CCR5 Left (L18) N1 N-term
    

    Use

    TALEN

    Tags

    3xFLAG and Fok1 EL variant

    Expression

    Mammalian

    Mutation

    K150Q

    Promoter

    CMV

    Available Since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. TALEN-CCR5R18-NtermN1

    Plasmid

    #51445

    Purpose

    Expresses TALEN targeting right CCR5A site (R18) with N1 N-terminal domain

    Depositor

    David Liu

    Article

    Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

    Insert

    TALEN CCR5 Right (R18) N1 N-term
    

    Use

    TALEN

    Tags

    3xFLAG and Fok1 KK variant

    Expression

    Mammalian

    Mutation

    K150Q

    Promoter

    CMV

    Available Since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGP-AAV-syn-iGABASnFR2n-WPRE

    Plasmid

    #218875

    Purpose

    AAV-mediated expression of improved GABA sensor (negative change in fluorescence)

    Depositor

    GENIE Project

    Article

    Kolb et al bioRxiv 2025.03.25.644953

    Insert

    iGABASnFR2n
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    S99A F104H R168P

    Promoter

    Synapsin

    Available Since

    May 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. bdSUMO-GFP-HiBit

    Plasmid

    #170989

    Purpose

    For bacterial expression of bdSUMO tagged muGFP with NanoLuc complementation peptide variant #86

    Depositor

    Angus Johnston

    Article

    Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

    Insert

    14x-His-bdSUMO-muGFP-HiBiT
    

    Use

    Luciferase

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV6-AC EJ7-GFP

    Plasmid

    #113617

    Purpose

    Reporter for canonical-NHEJ. Reporter for end joining between two double-strand breaks, induced with the 7a and 7b sgRNAs with CAS9. EJ between the distal ends w/o indel mutations restores GFP

    Depositor

    Jeremy Stark

    Article

    Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

    Insert

    EJ7-GFP variant of EGFP
    

    Expression

    Mammalian

    Available Since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. P445-FBP_17

    Plasmid

    #139708

    Purpose

    T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, Luz promoter is flower specific, circadian controlled

    Depositor

    Daniel Voytas

    Article

    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

    Insert

    pSlH4:NnCPH:tSlH4, pSlRBCS2:AsNpga:tAtuG7, pAtuMas:NnH3H:tAtuMas, p35S:NnHisps:t35S, pPhODO1:NnLuz:tAtuOcs
    

    Use

    Luciferase

    Expression

    Plant

    Promoter

    various

    Available Since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUAS-V5-TurboID-NES

    Plasmid

    #116904

    Purpose

    Expresses TurboID under Gal4/UAS control. TurboID is tagged at the N-terminus with V5 and a nuclear export signal (NES) at the C-terminus.

    Depositor

    Norbert Perrimon

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    TurboID
    

    Use

    Drosophila expression

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-hSyn-sbGLuc-B7-EYFP

    Plasmid

    #114110

    Purpose

    fusion protein of Gaussia luciferase variant slow burn, B7 transmembrane region, and enhanced yellow fluorescent protein; control for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Article

    Gomez-Ramirez et al J Neurosci Res. 2020 Mar;98(3):471-480.

    Inserts

    Gaussia luciferase, slow burn
    B7 hinge region
    Enhanced Yellow Fluorescent Protein
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. ClCry4 Y319D

    Plasmid

    #160649

    Purpose

    Expression of protein for various experiments including finding interaction partners

    Depositor

    Joseph Takahashi

    Article

    Zoltowski et al Proc Natl Acad Sci U S A. 2019 Sep 24;11

    Insert

    Clcry4 PHR Y319D
    

    Tags

    Hexa his

    Expression

    Bacterial

    Available Since

    Oct. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMALcPP-MS2cp

    Plasmid

    #203351

    Purpose

    Expresses His/MBP-tagged MS2-CP V75E/A81G variant in bacteria

    Depositor

    Thomas Cech

    Article

    Hemphill et al Proc Natl Acad Sci U S A. 2023 Jun 27;12

    Insert

    MS2 coat protein
    

    Tags

    Hx6-MBP

    Expression

    Bacterial

    Mutation

    V75E and A81G, to prevent capsid assembly

    Available Since

    July 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET22b_PstS_1

    Plasmid

    #78198

    Purpose

    Expression of single-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensor

    Depositor

    Martin Webb

    Article

    Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62

    Insert

    pstS (A197C)
    

    Expression

    Bacterial

    Mutation

    A197C (please see depositors comment below)

    Promoter

    T7

    Available Since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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21. Phi3-GCaMP6f

    Plasmid

    #106298

    Purpose

    CMV driven GFP based calcium (Ca2+) reporter

    Depositor

    Sanford Simon

    Article

    Belote et al J Cell Biol. 2020 Jan 6;219(1). pii: 132

    Insert

    GCaMP6f
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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22. pET22b_PstS_3

    Plasmid

    #78200

    Purpose

    Expression of double-cysteine variant of mature phosphate binding protein, PstS, which also has a mutation to weaken phosphate binding, as scaffold for phosphate biosensor

    Depositor

    Martin Webb

    Article

    Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62

    Insert

    pstS (A17C,I76G,A197C)
    

    Expression

    Bacterial

    Mutation

    A17C,I76G,A197C (please see depositor comment bel…

    Promoter

    T7

    Available Since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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23. MSCV Puro SCRIB WT

    Plasmid

    #88886

    Purpose

    SCRIB WT in MSCV backbone

    Depositor

    Senthil Muthuswamy

    Article

    Zheng et al Nat Cell Biol. 2016 Nov;18(11):1244-1252

    Insert

    SCRIB (SCRIB Human)
    

    Use

    Retroviral

    Tags

    N/A

    Mutation

    N/A

    Promoter

    MSCV promoter

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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24. pME minprom KalTa4

    Plasmid

    #195964

    Purpose

    Gateway pME Gal4 variant

    Depositor

    Alexa Burger, Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    mouse beta-globin minimal promoter KalTA4 (Gal4)
    

    Use

    Other

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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25. pGP-AAV-GFAP-iGABASnFR2-WPRE

    Plasmid

    #218871

    Purpose

    AAV-mediated expression of improved GABA sensor (positive change in fluorescence)

    Depositor

    GENIE Project

    Article

    Kolb et al bioRxiv 2025.03.25.644953

    Has Service

    AAV1 and AAV5

    Insert

    iGABASnFR2
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    S99A F102Y F104Y L178S

    Promoter

    GFAP

    Available Since

    May 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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26. AAV-hRedOpsin-TGFB1

    Plasmid

    #164427

    Purpose

    AAV plasmid expressing TGFB1 in photoreceptors

    Depositor

    Connie Cepko

    Article

    Wang et al J Clin Invest. 2020 Aug 3;130(8):4360-43

    Insert

    Tgfb1 (Tgfb1 Mouse)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    human red opsin

    Available Since

    Nov. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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27. pCAG-AsCas12a-Plus-mCherry

    Plasmid

    #191231

    Purpose

    To express the AsCas12a nuclease variant "AsCas12a-Plus" that enhanced the editing activity and specificity

    Depositor

    Zhili Rong

    Article

    Huang et al BMC Biol. 2022 Apr 25;20(1):91. doi: 10.

    Insert

    AsCas12a-Plus-P2A-mCherry
    

    Use

    CRISPR

    Mutation

    E174R, R951K, R955A

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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28. EP-Pol vector

    Plasmid

    #131686

    Purpose

    A lentiviral vector that constitutively expresses an error-prone variant of the adenovirus DNA polymerase

    Depositor

    Matthew D. Shoulders

    Article

    Berman et al J Am Chem Soc. 2018 Dec 26;140(51):18093

    Insert

    Adenovirus DNA polymerase
    

    Use

    Lentiviral

    Tags

    HA

    Mutation

    F421Y, D827A

    Available Since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET22b_PstS_2

    Plasmid

    #78199

    Purpose

    Expression of double-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensor

    Depositor

    Martin Webb

    Article

    Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62

    Insert

    pstS (A17C,A197C)
    

    Expression

    Bacterial

    Mutation

    A17C; A197C (please see depositor comment below)

    Promoter

    T7

    Available Since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGP-AAV-CAG-iGABASnFR2-WPRE

    Plasmid

    #218883

    Purpose

    AAV-mediated expression of improved GABA sensor (positive change in fluorescence)

    Depositor

    GENIE Project

    Article

    Kolb et al bioRxiv 2025.03.25.644953

    Insert

    iGABASnFR2
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    S99A F102Y F104Y L178S

    Promoter

    CAG

    Available Since

    June 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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