We narrowed to 59,254 results for: %s

1. pYeDP60-hATP13A2-WT

   Plasmid

   #171377

   Purpose

   A modified pYEDP60 plasmid containing a yeast codon-optimized version of human ATP13A2 variant 2, followed by a thrombin cleavage site and a C-terminal BAD tag. DOI: 10.21769/BioProtoc.3888

   Depositor

   Peter Vangheluwe

   Article

   van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:

   Insert

   human ATP13A2 variant 2 wild-type
   

   Tags

   BAD tag

   Expression

   Yeast

   Promoter

   URA3

   Available Since

   Sept. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. ABE8e-Cas9NG

   Plasmid

   #203328

   Purpose

   Plasmid for bacterial purification of codon optimized ABE8e-Cas9NG

   Depositor

   Vijay Sankaran

   Article

   Martin-Rufino et al Cell. 2023 Apr 27:S0092-8674(23)00332-X.

   Insert

   ABE8e-Cas9NG
   

   Use

   CRISPR

   Tags

   His-tag

   Expression

   Bacterial

   Available Since

   Oct. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. FLAG-FBXL17

   Plasmid

   #236188

   Purpose

   Expression of N-terminal tagged FLAG-FBXL17 (H. sapiens, codon-optimized) in human cell lines.

   Depositor

   Michele Pagano

   Article

   Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

   Insert

   FLAG-FBXL17 (FBXL17 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. H3K9me3 biosensor

   Plasmid

   #120802

   Purpose

   FRET biosensor. To monitor histone H3 lysine 9 tri-methylation in mammalian cells by FRET

   Depositor

   Yingxiao Wang

   Article

   Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

   Insert

   Truncated YPet-HP1-EV linker-ECFP-mouse histone H3
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCaggs-mGold2s

   Plasmid

   #231763

   Purpose

   Expression of mGold2s in mammalian cells

   Depositor

   Francois St-Pierre

   Article

   Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

   Insert

   mGold2s
   

   Expression

   Mammalian

   Mutation

   mGold2s is mGold with V1A;V22I;H77N;Q80R;K101E;D1…

   Promoter

   Actin promoter with CMV enhancer

   Available Since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. lenti-SpCas9 puro

   Plasmid

   #104994

   Purpose

   This 3rd generation lentiviral construct delivers hSpCas9 and puromycin resistance.

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Insert

   KpnI-XhoI-BsrGI-NheI
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCold-I-Dsup

   Plasmid

   #90021

   Purpose

   To express His-tagged Dsup in bacteria

   Depositor

   Takekazu Kunieda

   Article

   Hashimoto et al Nat Commun. 2016 Sep 20;7:12808. doi: 10

   Insert

   Dsup
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   cspA

   Available Since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-G-Flamp1-mut

   Plasmid

   #188568

   Purpose

   control probe of G-Flamp1, insensitive to cAMP

   Depositor

   Jun Chu

   Article

   Wang et al Nat Commun. 2022 Sep 12;13(1):5363. doi:

   Insert

   G-Flamp1-mut
   

   Tags

   His tag

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-OptoSTIM1

   Plasmid

   #70159

   Purpose

   Mammalian expression plasmid of OptoSTIM1 (optogenetically-activated STIM1 protein)

   Depositor

   Won Do Heo

   Article

   Kyung et al Nat Biotechnol. 2015 Sep 14. doi: 10.103

   Insert

   OptoSTIM1 (STIM1 Human, Mustard Weed)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-2xFLAG-2xSTREP-CCND2

    Plasmid

    #172629

    Purpose

    Expresses 2xFLAG-2xSTREP-tagged cyclin D2 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND2 (CCND2 Human)
    

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEF6-CSPG4-myc-his

    Plasmid

    #69037

    Purpose

    Expression of CSPG4 in mammalian cells

    Depositor

    Wensheng Wei

    Article

    Yuan et al Cell Res. 2015 Feb;25(2):157-68. doi: 10

    Insert

    CSPG4 (CSPG4 Human)
    

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1 alpha

    Available Since

    Sept. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pF CAG luc IRES neo

    Plasmid

    #98294

    Purpose

    For constitutive expression of firefly luciferase from the CAG promoter together with an IRES-linked neo resistance gene

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Inserts

    Firefly luciferase
    IRES
    Neomycin resistance gene (NeoR)
    

    Use

    Lentiviral and Luciferase

    Available Since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLentiSIV hSyn HA-NLS-SpCas9-NLS WPRE

    Plasmid

    #236242

    Purpose

    Lentiviral expression of SpCas9

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    SpCas9
    

    Use

    Lentiviral

    Promoter

    human Synapsin 1

    Available Since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. DDX21

    Plasmid

    #155510

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    DDX21 (DDX21 Human)
    

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    T232A

    Promoter

    EF-1a

    Available Since

    Sept. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV2-hSyn-G-Flamp1-mut

    Plasmid

    #188570

    Purpose

    control probe of G-Flamp1, insensitive to cAMP

    Depositor

    Jun Chu

    Article

    Wang et al Nat Commun. 2022 Sep 12;13(1):5363. doi:

    Insert

    G-Flamp1-mut
    

    Use

    AAV

    Tags

    His tag

    Promoter

    hSyn

    Available Since

    Oct. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-mGold2t-CAF1-C-10

    Plasmid

    #231782

    Purpose

    Visualization of nucleus in mammalian cells using mGold2t

    Depositor

    Francois St-Pierre

    Article

    Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

    Insert

    mGold2t-CAF1-C-10 (Chaf1a Mouse)
    

    Tags

    mGold2s

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. eTd-CBE

    Plasmid

    #196601

    Purpose

    Mammalian expression of eTd-CBE

    Depositor

    Dali Li

    Article

    Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103

    Insert

    bpNLS-TadA-8e (N46L)-nSpCas9-bpNLS-P2A-UGI-bpNLS
    

    Tags

    6*His

    Expression

    Mammalian

    Available Since

    March 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. sgAPC(A)

    Plasmid

    #85557

    Purpose

    Expresses sgRNA targeting human APC around codon I1417

    Depositor

    Luca Grumolato

    Article

    Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

    Insert

    APC, WNT signaling pathway regulator (APC Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

19. pLenti-EF-FH-TAZ S89A-ires-blast

    Plasmid

    #52084

    Purpose

    Lentiviral expression vector for TAZ S89A mutant with N-terminal FLAG and His tags

    Depositor

    Yutaka Hata

    Article

    Yang et al Mol Cell Biol. 2014 Feb 18.

    Insert

    TAZ S89A (WWTR1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG and His

    Expression

    Mammalian

    Mutation

    S89A

    Promoter

    EF1a

    Available Since

    April 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-HA-CCND3

    Plasmid

    #172621

    Purpose

    Expresses HA-tagged cyclin D3 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND3 (CCND3 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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