Addgene

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pAG364-greenFAST

Plasmid

#160353

Purpose

expresses greenFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

greenFAST

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pAG365-redFAST

Plasmid

#160354

Purpose

Expresses redFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

redFAST

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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FR_HNF6

Plasmid

#31099

Depositor

Gerhart Ryffel

Article

Thomas et al Nucleic Acids Res. 2004 . 32(19):e150.

Insert

HNF6 (ONECUT1 Human)

Use

Tags

His

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pCMV-G-Cdc42

Plasmid

#180577

Purpose

ddGFP sensor of Cdc42

Depositor

Won Do Heo

Article

Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

Insert

ddGFP sensor of Cdc42

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPE-600n

Plasmid

#13537

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E WT

Use

Tags

ECFP and Venus

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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4xMS2-hTR

Plasmid

#154940

Purpose

4xMS2 stem-loop-hTR-500 bp genomic sequence

Depositor

Alice Ting

Article

Han et al Proc Natl Acad Sci U S A. 2020 Aug 24. p

Insert

4xMS2 stem-loop-hTR-500 bp genomic sequence

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-Cas9-EGFP

Plasmid

#159126

Purpose

Expresses active Cas9 fused with EGFP for fluorescence microscopy

Depositor

Taekjip Ha

Article

Liu et al Science. 2020 Jun 12;368(6496):1265-1269

Insert

Cas9 fused to EGFP

Use

CRISPR and Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

MSCV LTR promoter

Available since

Dec. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pUREF-EX

Plasmid

#199331

Purpose

A pEF-BOS-EX-derived expression vector that contains an SV40 promoter-driven puromycin resistance gene

Depositor

Rikiro Fukunaga

Article

Fujii et al Genes Cells. 2023 Mar 2. doi: 10.1111/gt

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pLCS104

Plasmid

#209357

Purpose

Gateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter for N-terminal HaloTag fusion protein.

Depositor

Lucia Strader

Article

Allen et al Res Sq. 2023 Nov 7:rs.3.rs-3558280. doi:

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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Orai1 CFP

Plasmid

#19757

Depositor

Anjana Rao

Article

Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

Insert

Orai1 (ORAI1 Human)

Use

Tags

CFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2008

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-nmdCas9-3XGFP

Plasmid

#64109

Purpose

Expression of Nm dCas9-3XGFP in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Nm dCas9

Use

CRISPR and Lentiviral

Tags

3XsfGFP

Expression

Mammalian

Mutation

Promoter

CMV-TO

Available since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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pLCS105

Plasmid

#209358

Purpose

Gateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter for N-terminal Dendra2 fusion protein.

Depositor

Lucia Strader

Article

Allen et al Res Sq. 2023 Nov 7:rs.3.rs-3558280. doi:

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pLCS101

Plasmid

#209355

Purpose

Gateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter for N-terminal mVenus fusion protein.

Depositor

Lucia Strader

Article

Allen et al Res Sq. 2023 Nov 7:rs.3.rs-3558280. doi:

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pLCS107

Plasmid

#209328

Purpose

Gateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter for N-terminal mNeonGreen fusion protein.

Depositor

Lucia Strader

Article

Allen et al Res Sq. 2023 Nov 7:rs.3.rs-3558280. doi:

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pSLC-246

Plasmid

#73190

Purpose

Template plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (tse2) under the control of rhamnose induceable promoter (PrhaB) for negative selection.

Depositor

Swaine Chen

Article

Khetrapal et al Nucleic Acids Res. 2015 Jul 27;43(13):e8

Insert

PrhaB-tse2

Use

Tags

Expression

Bacterial

Mutation

Promoter

PrhaB

Available since

July 15, 2016

Availability

Academic Institutions and Nonprofits only

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pFlag-CMV2-Smyd2

Plasmid

#13521

Depositor

Shelley Berger

Article

Huang et al Nature. 2006 Nov 30. 444(7119):629-32.

Insert

Smyd2 (Smyd2 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 1, 2007

Availability

Academic Institutions and Nonprofits only
pRSET FLIPPi-30m

Plasmid

#13556

Depositor

Wolf Frommer

Article

Gu et al FEBS Lett. 2006 Oct 30. 580(25):5885-93.

Insert

FLIPPi T22A

Use

Tags

ECFP and EYFP

Expression

Bacterial

Mutation

T22A in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pWaldo-GFPd_PepTSt

Plasmid

#58333

Purpose

Expresses the POT family transporter from Streptococcus thermophilus in E. coli as a C-terminally tagged GFP his tagged protein

Depositor

Simon Newstead

Article

Solcan et al EMBO J. 2012 Aug 15;31(16):3411-21. doi:

Insert

PepTSt

Use

Tags

GFP-His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only
U6-(BbsI)sgRNA_CAG-Venus-bpA

Plasmid

#86985

Purpose

For cloning and expression of sgRNA together with Venus expression

Depositor

Ralf Kuehn

Article

Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

Insert

Venus

Use

Tags

Expression

Mammalian

Mutation

Venus GFP variant

Promoter

CAG

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pRC3EGFP

Plasmid

#193790

Purpose

Expresses EGFP under the control of PR promoter, p15A origin of replication, Chloramphenicol selection

Depositor

Sangram Bagh

Article

Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

Insert

EGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

PR promoter

Available since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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