We narrowed to 12,339 results for: pcdna

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rASAP

Plasmid

#178205

Purpose

Ratiometric version of the genetically encoded voltage indicator ASAP2s-I67T-Q397R.

Depositor

Stefan Heinemann

Article

Ruhl et al Commun Biol. 2021 Oct 7;4(1):1164. doi:

Insert

rASAP

Expression

Mammalian

Available Since

Feb. 1, 2022

Availability

Academic Institutions and Nonprofits only

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PAC_E83_LOV_eGFP

Plasmid

#231574

Purpose

This plasmid mediates puromycin resistance in the dark, resulting in puro mediated cell death upon illumination with blue light

Depositor

Dominik Niopek

Article

Wolf et al bioRxiv 2024.12.04.626757

Insert

PAC-P2A-GFP carrying an insertion of AsLOV2 behind E83

Expression

Mammalian

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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HA-MCPIP1

Plasmid

#127459

Purpose

Expressing human MCPIP1 in mammalian cells

Depositor

Mingui Fu

Article

Liang et al J Exp Med. 2010 Dec 20;207(13):2959-73.

Insert

MCPIP1 (ZC3H12A Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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Shp2 biosensor

Plasmid

#134346

Purpose

FRET biosensor. To visualize Shp2 activity by FRET

Depositor

Yingxiao Wang

Article

Sun et al Nat Commun. 2017 Sep 7;8(1):477. doi: 10

Insert

Shp2 Biosensor

Expression

Mammalian

Available Since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

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HA-Mob

Plasmid

#32835

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

Mob (MOB1A Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 15, 2011

Availability

Academic Institutions and Nonprofits only

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BirA-Myc_N_MYOD1

Plasmid

#167716

Purpose

Expresses MYOD1 in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

MYOD1 (MYOD1 Human)

Tags

Myc-BirA

Expression

Mammalian

Mutation

no mutations

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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pMyosin-miRFP670nano3

Plasmid

#184668

Purpose

LC-Myosin labelled with near-infrared fluorescent protein miRFP670nano3

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

miRFP670nano3-LC-Myosin

Tags

miRFP670nano3

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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SB52

Plasmid

#21487

Depositor

Shoumo Bhattacharya

Article

Bhattacharya et al Genes Dev. 1999 Jan 1. 13(1):64-75.

Insert

Cited2 (CITED2 Human)

Expression

Mammalian

Available Since

July 23, 2009

Availability

Academic Institutions and Nonprofits only
pFH2.12_GagZip-MCP-P2A-eGFP

Plasmid

#205527

Purpose

Express HIV GagZip-MCP-P2A-eGFP

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

GagZip-MCP

Expression

Mammalian

Mutation

Nucleocapsid replaced with leucine zipper

Promoter

CMV

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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HA-Cer-NCreIntN

Plasmid

#160505

Purpose

Expresses NCreIntN, tagged with HA, in mammalian cells in split intein-mediated split-Cre recombinase applications

Depositor

Shawn Je

Article

Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

Insert

NCreIntN

Use

Cre/Lox

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pspCas9-HF-tetra-com-vector

Plasmid

#132555

Purpose

Vector plasmid expressing high fidelity spCas9 (R691A) and sgRNA scaffold with com replacing the Tetraloop.

Depositor

Baisong Lu

Article

Lu et al CRISPR J. 2021 Mar 16. doi: 10.1089/cris

Inserts

High fidelity Cas9
sgRNA scaffold with com replacing the Tetraloop

Expression

Mammalian

Available Since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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HA-AMOT p130 Y242/287A

Plasmid

#32822

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2011 Jan 1;25(1):51-63.

Insert

AMOT p130 (AMOT Human)

Tags

HA

Expression

Mammalian

Mutation

E5D, Y242/287A

Promoter

CMV

Available Since

Jan. 11, 2012

Availability

Academic Institutions and Nonprofits only
p3VEA3

Plasmid

#234796

Purpose

Mammalian expression of bovine arrestin-3

Depositor

Vsevolod Gurevich

Article

Zheng et al Cells. 2023 Jun 6;12(12):1563. doi: 10.3

Insert

Arrestin-3 (ARR3 Bovine)

Tags

Venus

Expression

Mammalian

Mutation

none

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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mc2 neo

Plasmid

#206214

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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Plasma membrane-targeted optodPLD

Plasmid

#140061

Purpose

Encodes CRY2-mCherry-dPLD-P2A-CIBN-CAAX for mammalian expression

Depositor

Jeremy Baskin

Article

Tei et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Phospholipase D

Tags

CAAX, CIBN, CRY2, P2A, and mCherry

Expression

Mammalian

Mutation

H170A (catalytic dead)

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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4xHRE_minCMV-sfGFP-CMV_dsRed Exp

Plasmid

#117401

Purpose

sfGFP expressed from hypoxia-inducible minimal CMV promoter and dsRed expressed from consitutive CMV promoter

Depositor

Yvonne Chen

Article

Ede et al ACS Synth Biol. 2016 May 20;5(5):395-404

Insert

SuperFolder GFP

Expression

Mammalian

Available Since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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STAGR_gRNAScaffold_h7SK

Plasmid

#102842

Purpose

Can be used as PCR template for a STAgR reaction

Depositor

Stefan Stricker

Article

Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

Insert

gRNAScaffold_h7SKpromoter

Use

As pcr template

Promoter

h7SK

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-MRGBP

Plasmid

#15325

Depositor

Joan Conaway, Ronald Conaway

Article

Cai et al J Biol Chem. 2003 Oct 31. 278(44):42733-

Insert

MRG-binding protein (MRGBP Human)

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only
HA.PKD.K/W

Plasmid

#10809

Depositor

Alex Toker

Article

Storz et al J Biol Chem. 2003 May 16. 278(20):17969-

Insert

PKD (PRKD1 Human)

Tags

HA and Myc (inserted between amino acids 33&3…

Expression

Mammalian

Mutation

Kinase dead mutant: K612W (Lys 612 to Trp) (bp183…

Promoter

CMV

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only
inert_EJC

Plasmid

#216224

Purpose

GFP P2A RFP reporter with inert EJC immediately following stop codon

Depositor

Rebecca Voorhees

Article

Inglis et al J Cell Sci. 2023 May 15;136(10):jcs26121

Insert

GFP P2A RFP_inert_EJC

Expression

Mammalian

Available Since

April 15, 2024

Availability

Academic Institutions and Nonprofits only

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pFH2.30_Gag-MCP-dpol-T2A-eGFP

Plasmid

#205538

Purpose

Express HIV Gag-MCP-dpol-T2A-eGFP

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

HIV Gag-MCP-dpol

Expression

Mammalian

Mutation

pol frameshift sequence recoded

Promoter

CMV

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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mSIRT3-L

Plasmid

#33309

Depositor

Michael Sack

Article

Bao et al J Cell Biochem. 2010 May;110(1):238-47.

Insert

Sirt3 (Sirt3 Mouse)

Tags

3XFlag

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2012

Availability

Academic Institutions and Nonprofits only

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TRPML1-CFP

Plasmid

#18827

Depositor

Craig Montell

Article

Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

Insert

TRPML1 (MCOLN1 Human)

Tags

CFP

Expression

Mammalian

Available Since

July 14, 2008

Availability

Academic Institutions and Nonprofits only
M-ST1n-VP64

Plasmid

#48675

Purpose

Mammalian ST1-VP64 nuclease-null Cas9 activator expression, human optimized

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Cas9-VP64, nuclease-null

Use

CRISPR

Tags

NLS and VP64

Mutation

nuclease-null (D9A D598A H599A N622A)

Promoter

CMV

Available Since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

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nls-Flamindo2

Plasmid

#73939

Purpose

Expresses biosensor for cAMP in mammalian cells with nuclear localization signal

Depositor

Tetsuya Kitaguchi

Article

Odaka et al PLoS One. 2014 Jun 24;9(6):e100252. doi:

Insert

nls-Flamindo2

Expression

Mammalian

Promoter

CMV

Available Since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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pmyc-GFP-TNRC6B

Plasmid

#42022

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6B (TNRC6B Human)

Tags

EGFP and Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 5, 2013

Availability

Academic Institutions and Nonprofits only

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Mammalian_SpCas9_Target-AID2S

Plasmid

#188648

Purpose

Expresses SpCas9_Target-AID2S in mammalian cells

Depositor

Keiji Nishida

Article

Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

Insert

nCas9-AID2S-UGI

Tags

UGI

Expression

Mammalian

Mutation

D10A for SpCas9

Promoter

pCMV

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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mc2 mNeon hPGK

Plasmid

#206230

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-Rubicon deltaCT

Plasmid

#28040

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

Insert

Rubicon (RUBCN Human)

Tags

3xFlag and EGFP

Expression

Mammalian

Mutation

delta880-end

Available Since

March 22, 2011

Availability

Academic Institutions and Nonprofits only

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BirA-Myc_N_TAL1

Plasmid

#167728

Purpose

Expresses TAL1 in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

TAL1 (TAL1 Human)

Tags

Myc-BirA

Expression

Mammalian

Mutation

no mutations

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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