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Showing: 6621 - 6640 of 65941 results

  6. Cas9/pTREX-n

    Plasmid
    #68708
    Purpose
    Expresses the fusion gene CAS9-HA-2xNLS-GFP in the pTREX-n backbone. This vector is used for cloning a specific sgRNA by BamHI, to be co-expressed with Cas9 for genome editing in Trypanosoma cruzi.
    Depositor
    Roberto Docampo
    Publication
    Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 (Addgene) .
    Use
    CRISPR
    Tags
    Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 …
    Mutation
    C4239T mutation that eliminates a BamHI restricti…
    Available since
    Sept. 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    11 Articles
    68708-plasmid-map-sequence-id-123989

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  8. Ii-Str_SBP-EGFP-Ecadherin

    Plasmid
    #65288
    Purpose
    synchronize trafficking of Ecadherin from the ER (RUSH system)
    Depositor
    Franck Perez
    Publication
    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ecadherin
    Tags
    EGFP and IL-2 Signal Sequence and Streptavidin Bi…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    65288-plasmid-map-sequence-id-120704

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  9. MSP1673

    Plasmid
    #65769
    Purpose
    Bacterial expression plasmid for S. thermophilus1 Cas9 & sgRNA (need to clone in spacer into BspMI sites): T7-humanSt1Cas9-NLS-T7-BspMIcassette-St1-sgRNA
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS, and St1Cas9 gRNA
    Use
    CRISPR
    Tags
    NLS
    Expression
    Bacterial
    Promoter
    T7 (x2)
    Available since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    65769-plasmid-map-sequence-id-112659

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  13. MAVS-Pex

    Plasmid
    #44557
    Purpose
    Retroviral vector for expression of a chimeric MAVS allele targeted to peroxisomes in mammalian cells
    Depositor
    Jonathan Kagan
    Publication
    Dixit et al Cell. 2010 May 14;141(4):668-81. Epub 20
    Insert
    MAVS-Pex13 chimeric protein (MAVS Human)
    Use
    Retroviral
    Expression
    Mammalian
    Mutation
    contains aa1-500 of MAVS and aa136-233 of Pex13
    Available since
    Aug. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles

  19. pSH62-EBD

    Plasmid
    #49455
    Purpose
    yeast expression of Cre recombinase fused to the estrogen binding domain (EBD), which confers estradiol-dependent control to Cre recombinase
    Depositor
    Marc Gartenberg
    Publication
    Cheng et al Nucleic Acids Res. 2000 Dec 15;28(24):E1
    Insert
    Cre-EBD fusion
    Use
    Cre/Lox
    Expression
    Yeast
    Promoter
    Gal1 promoter
    Available since
    Dec. 5, 2013
    Availability
    Academic Institutions and Nonprofits only
    49455-plasmid-map-sequence-id-73668

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Showing: 6621 - 6640 of 65941 results