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pRSET FLIPPi-200u

Plasmid

#13555

Depositor

Wolf Frommer

Article

Gu et al FEBS Lett. 2006 Oct 30. 580(25):5885-93.

Insert

FLIPPi G162A

Use

Tags

ECFP and EYFP

Expression

Bacterial

Mutation

G162A in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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PAX6 donor plasmid

Plasmid

#105239

Purpose

Knock in H2B-GFP into the human PAX6 locus

Depositor

Lorenz Studer

Article

Tchieu et al Cell Stem Cell. 2017 Sep 7;21(3):399-410

Insert

PAX6 Homologous recombination ARMS with a P2A-H2B-GFP and Puromycin selection cassette

Use

TALEN

Tags

Expression

Mutation

Promoter

Available since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-G-Kras

Plasmid

#180571

Purpose

ddGFP sensor of Kras

Depositor

Won Do Heo

Article

Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

Insert

ddGFP sensor of Kras

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-Gdf2

Plasmid

#163595

Purpose

Expresses GDF-2 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Gdf2 (Gdf2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pBx-Spas-sgRNA-Gm

Plasmid

#105232

Purpose

Plasmid containing S. pasteurianus sgRNA with no 20bp target (Empty control) Gentamycin marker for P. aeruginosa

Depositor

Kristala Prather

Article

Tan et al J Bacteriol. 2018 Mar 12;200(7). pii: JB

Insert

S. pasteurianus sgRNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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pFU-MVIIA-PC

Plasmid

#24148

Depositor

Ines Ibanez-Tallon

Article

Auer et al Nat Methods. 2010 Feb 7. ():.

Insert

omega-Conotoxin MVIIA-FLAG-PDGF_R_TM-mCherry (PDGFRB Human, Conus magus, Aequorea victoria)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2010

Availability

Academic Institutions and Nonprofits only

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pUC18-mini-Tn7T-Plac-dCas9 (Plac)

Plasmid

#105235

Purpose

Tn7 integrating plasmid with S. pasteurianus dCas9 expressed from the Plac promoter for expression in P. aeruginosa

Depositor

Kristala Prather

Article

Tan et al J Bacteriol. 2018 Mar 12;200(7). pii: JB

Insert

S. pasteurianus dCas9

Use

CRISPR and Synthetic Biology

Tags

HA

Expression

Bacterial

Mutation

D10A, H599A

Promoter

Available since

Aug. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pPOTv6-puro-3Ty:mNG:3Ty

Plasmid

#179798

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Keith Gull, Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mNG

Use

Unspecified

Tags

Expression

Mutation

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pQCasTns(Ptr)-IS186

Plasmid

#175581

Purpose

Expression of CRISPR-associated transposases

Depositor

Sheng Yang

Article

Yang et al Nucleic Acids Res. 2021 Sep 3. pii: 6363

Insert

PtrTniQ, PtrCas5/8, PtrCas7, PtrCas6, PtrTnsABC, CRISPR(IS186)

Use

Tags

Expression

Bacterial

Mutation

NO

Promoter

Available since

Jan. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAG461-FRB-redNFAST

Plasmid

#160362

Purpose

Expresses FRB-redNFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

FRB-redNFAST

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pDN-LexAOx4-mNeonGreen

Plasmid

#192604

Purpose

Vector for inducible mNeonGreen expression with 4x LexA operator (LexAO)

Depositor

Yingxiao Wang

Article

Huang et al Sci Adv. 2020 Feb 19;6(8):eaay9209. doi:

Insert

mNeonGreen

Use

Unspecified

Tags

Expression

Mutation

Promoter

Minimal promoter

Available since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPPi-5u

Plasmid

#13554

Depositor

Wolf Frommer

Article

Gu et al FEBS Lett. 2006 Oct 30. 580(25):5885-93.

Insert

FLIPPi S52A

Use

Tags

ECFP and EYFP

Expression

Bacterial

Mutation

S52A in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pEiLOV-N1

Plasmid

#26588

Depositor

John Christie

Article

Chapman et al Proc Natl Acad Sci U S A. 2008 Dec 16. 1

Insert

iLOV

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 28, 2010

Availability

Academic Institutions and Nonprofits only

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pCAG-Gdf6

Plasmid

#163598

Purpose

Expresses GDF-6 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Gdf6 (Gdf6 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-FLAG-LOV*-H2B

Plasmid

#202204

Purpose

Expresses the photoproximity labeling catalyst LOV* fused to H2B in mammalian cells

Depositor

Thomas Muir

Article

Hananya et al Proc Natl Acad Sci U S A. 2023 Apr 18;12

Insert

Histone H2B type 1-K

Use

Tags

FLAG and LOV*

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-mNG2(1-10)

Plasmid

#190209

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mNeonGreen2(1-10)

Use

Unspecified

Tags

Expression

Mutation

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAG460-FRB-greenNFAST

Plasmid

#160361

Purpose

Expresses FRB-greenNFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

FRB-greenNFAST

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-Flp-DOG

Plasmid

#80084

Purpose

Flp recombinase dependent on GFP (Flp-DOG) for expression in mammalian cells

Depositor

Connie Cepko

Article

Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

Insert

Flp-DOG

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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SpyTag003-mClover3

Plasmid

#133453

Purpose

Expresses SpyTag003-mClover3 (a green fluorescent protein) in bacterial cytoplasm

Depositor

Mark Howarth

Article

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyTag003-mClover3

Use

Tags

GSGSGSGS linker and His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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AcrIIA4

Plasmid

#101043

Purpose

Anti-CRISPRprotein which inhibit SpyCas9 and LmoCas9 activity.

Depositor

Zhiwei Huang

Article

Dong et al Nature. 2017 Jun 15;546(7658):436-439. d

Insert

AcrIIA4

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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