We narrowed to 7,847 results for: ALP

Showing: 6621 - 6640 of 7847 results

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pWZ403

Plasmid

#208113

Purpose

mSwAP-In marker cassette 1 with mScarlet-PuroTK

Depositor

Jef Boeke

Article

Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-

Insert

mScarlet-PuroTK

Tags

P2A

Expression

Bacterial and Mammalian

Promoter

EF1alpha

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pAW-NbVHH05-mCherry

Plasmid

#171572

Purpose

Expresses mCherry-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-mCherry) in fly cells

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

NbVHH05-mCherry (UBE2J1 Human)

Tags

mCherry

Expression

Insect

Promoter

fly actin5C promoter

Available Since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-n1-AICD

Plasmid

#69925

Purpose

mammalian expression of human AICD fused to EGFP

Depositor

Zita Balklava, Thomas Wassmer

Article

Currinn et al Cell Mol Life Sci. 2015 Jul 28.

Insert

AICD (APP Human)

Tags

EGFP

Expression

Mammalian

Mutation

47 aa long

Promoter

CMV

Available Since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only

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pMK321 (TOP2A-mAC (Neo))

Plasmid

#140652

Purpose

TOP2A tagging with mAID-Clover

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

TOP2A (TOP2A Human)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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betaTrCP mVenus reporter

Plasmid

#192458

Purpose

Fluorescent reporter for beta-TrCP activity in human cells

Depositor

Steven Cappell

Article

Paul et al Nat Commun. 2022 Oct 26;13(1):6364. doi:

Insert

CDC25B (CDC25B Human)

Use

Lentiviral

Tags

SV40 NLS and mVenus

Expression

Mammalian

Mutation

aa198-338

Promoter

EF1alpha

Available Since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pJM31

Plasmid

#52986

Purpose

A secreted Annexin V (sAnxV::GFP) Phosphatidylserine sensor

Depositor

Ding Xue

Article

Mapes et al Curr Biol. 2012 Jul 24;22(14):1267-75. d

Insert

Annexin V (ANXA5 Human)

Tags

GFP (w/ introns) and signal sequence

Expression

Worm

Promoter

hsp-16.41

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pLV[Exp]-Neo-EF1A>Tet3G

Plasmid

#184379

Purpose

The Tet3G gene is expressed under a constitutive EF1-alpha promoter. This protein binds a TRE3G promoter to activate gene transcription only in the presence of tetracycline or its analogs (e.g. doxycycline)

Depositor

Ori Bar-Nur

Article

Domenig et al Stem Cell Reports. 2022 Feb 8;17(2):321-

Insert

Tet3G

Use

Lentiviral

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pJR107

Plasmid

#187245

Purpose

Lentiviral sgRNA vector for CROP-seq with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-GFP marker expression

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Lentiviral sgRNA vector for CROP-seq with mU6 sgRNA promoter

Use

Lentiviral

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-DIO-mCReP

Plasmid

#167503

Purpose

Conditional expression of the eIF2alpha phophatase, CReP (Ppp1r15b)

Depositor

Nicole Calakos

Article

Helseth et al Science. 2021 Apr 23;372(6540). pii: 372

Insert

Ppp1r15b (Ppp1r15b Mouse)

Use

AAV and Cre/Lox

Tags

Myc

Expression

Mammalian

Promoter

hSyn

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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pAW-NbVHH05-GFP

Plasmid

#171570

Purpose

Expresses GFP-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-GFP) in fly cells

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

NbVHH05-GFP (UBE2J1 Human)

Tags

GFP

Expression

Insect

Promoter

fly actin5C promoter

Available Since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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pJR106

Plasmid

#187244

Purpose

Lentiviral sgRNA vector for CROP-seq with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-BFP marker expression

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Lentiviral sgRNA vector for CROP-seq with mU6 sgRNA promoter

Use

Lentiviral

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pWZ852

Plasmid

#208114

Purpose

mSwAP-In marker cassette 1 with mScarlet-HygTK

Depositor

Jef Boeke

Article

Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-

Insert

mScarlet-HygTK

Tags

T2A

Expression

Bacterial and Mammalian

Promoter

EF1alpha

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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p23-puro-EGFP-FBL

Plasmid

#179235

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Wu et al Science. 2021 Jul 30;373(6554):547-555.

Insert

FBL (FBL Human)

Tags

EGFP

Expression

Mammalian

Promoter

EF-1alpha

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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p1.2-Hyg-Fur

Plasmid

#162774

Purpose

Soluble human PACE/furin protease expression

Depositor

Ivan Vorobiev

Article

Kovnir et al Acta Naturae. 2018 Jan-Mar;10(1):51-65.

Insert

Human PACE/furin protease soluble (FURIN Human)

Expression

Mammalian

Promoter

CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac_His-ZZ-TEV-Lis1-SNAPf

Plasmid

#133073

Purpose

Plasmid for insect cell expression of human full-length LIS1 with an N terminal His-ZZ-LTLT tag and C terminal SNAPf tag

Depositor

Simon Bullock, Andrew Carter

Article

Baumbach et al Elife. 2017 Apr 13;6. pii: e21768. doi:

Insert

Lis1 (PAFAH1B1 Human)

Tags

8x His, Linker-TEV-linker_TEV, SNAPf, and ZZ

Expression

Insect

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pMJA285= pSin-TCRab-CMVp-Puro

Plasmid

#116875

Purpose

stable TCR expression in human T cells

Depositor

Marcos Alcocer

Article

Wang et al Biotechnol Lett. 2019 Oct;41(10):1095-11

Insert

TCR (TRAV10, TRBV25-1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

deletion of EF1-a promotor

Promoter

CMV Promotor

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pSKC12 - DelQ

Plasmid

#110493

Purpose

Monitor the expression of Luciferase driven by a mutated NRAS 5'-UTR.

Depositor

Shankar Balasubramanian

Article

Kumari et al Nat Chem Biol. 2007 Apr;3(4):218-21. doi

Insert

NRAS 5'- DelQ (bp 30-254) (NRAS Human)

Use

Luciferase

Tags

Firefly luciferase

Mutation

Deletion of the first 29 base pairs of the NRAS 5…

Promoter

T7

Available Since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-sCre

Plasmid

#55635

Purpose

Expresses sCre in Mammalian Cells

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

Cre

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EF1alpha

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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pSP64-hKCNQ1

Plasmid

#53048

Purpose

Expresses alpha subunit for hKCNQ1 channel in Xenopus oocytes

Depositor

Michael Sanguinetti

Article

Sanguinetti et al Nature. 1996 Nov 7;384(6604):80-3.

Insert

Potassium voltage-gated channel subfamily KQT member 1 (KCNQ1 Human)

Use

Xenopus oocyte expression

Promoter

SP6

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only

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pAG43

Plasmid

#226736

Purpose

Expresses a reporter for an alpha-helical OMM protein

Depositor

Jonathan Weissman

Article

Guna et al Science. 2022 Oct 21;378(6617):317-322.

Insert

MICU1 (targeting sequence) (MICU1 Human)

Use

Lentiviral

Tags

GFP11

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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