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pKG-GW1

Plasmid

#203163

Purpose

Yeast expression vector; To express proteins under control of TDH3 promoter and terminator using leucine selection

Depositor

William Sessa

Article

Park et al Cell Metab. 2014 Sep 2;20(3):448-57. doi

Type

Empty backbone

Expression

Yeast

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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gh90

Plasmid

#106761

Purpose

expression of gRNA targeting FUT6

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

FUT6 (FUT6 Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCV-SMYD2 F184A

Plasmid

#85659

Purpose

retroviral expression of human SMYD2 F184A

Depositor

Julien Sage

Article

Reynoird et al Genes Dev. 2016 Apr 1;30(7):772-85. doi:

Insert

SMYD2 (SMYD2 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

F184A

Promoter

LTR

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pDRf1-4CL5-AtSCT (JBEI-11557)

Plasmid

#87937

Purpose

Co-expression in S.cerevisiae of 4CL5 and AtSCT

Depositor

Dominique Loque

Article

Eudes et al Microb Cell Fact. 2016 Nov 21;15(1):198.

Inserts

AtSCT
4CL5 (4CL5 Mustard Weed)

Expression

Yeast

Available Since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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GST-GAR

Plasmid

#34697

Depositor

Steven Clarke

Article

Frankel et al J Biol Chem. 2000 Oct 20;275(42):32974-8

Insert

Fibrillarin (FBL Human)

Tags

GST

Expression

Bacterial

Mutation

Residues 1-145 of human fibrillarin with K2E and …

Promoter

tac

Available Since

July 12, 2012

Availability

Academic Institutions and Nonprofits only
pGEX-HCF-1rep1E10A

Plasmid

#84607

Purpose

Plasmid contains HCF-1rep1E10A with GST-tag

Depositor

Winship Herr

Article

Capotosti et al Cell. 2011 Feb 4;144(3):376-88. doi: 10.

Insert

HCF-1rep1E10A (HCFC1 Human)

Tags

GST and His

Expression

Bacterial

Promoter

T7

Available Since

Sept. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX-HCF-1rep1wt

Plasmid

#84606

Purpose

Plasmid contains HCF-1rep1wt with GST-tag

Depositor

Winship Herr

Article

Capotosti et al Cell. 2011 Feb 4;144(3):376-88. doi: 10.

Insert

HCF-1rep1wt (HCFC1 Human)

Tags

GST-TEV and His

Expression

Bacterial

Promoter

T7

Available Since

Sept. 18, 2017

Availability

Academic Institutions and Nonprofits only

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(282) pcDNA3.1-HA-nGFP-(EAAAK)4-OGT(4,K852A)

Plasmid

#160741

Purpose

Expresses an inactive truncated OGT (4.5 TPRs, K852A) that can't bind O-GlcNAc fused to a GFP nanobody

Depositor

Christina Woo

Article

Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

Insert

HA-nGFP-(EAAAK)4-OGT(4,K852A)

Tags

HA-Tag

Expression

Mammalian

Promoter

T7/CMV

Available Since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 MYC dLZ

Plasmid

#176048

Purpose

Expresses Myc Mutant (delta leucine zipper) in Mammalian cells

Depositor

Linda Penn

Article

Tu et al Cancer Cell. 2018 Oct 8;34(4):579-595.e8

Insert

Myc

Expression

Mammalian

Mutation

deleted amino acids 414-433

Promoter

CMV

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pDSHA-ARL2-Myc

Plasmid

#67354

Purpose

Mammalian expression of hArl2 with C-term Myc tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL2 (ARL2 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDSHA-ARL8A-Myc

Plasmid

#67361

Purpose

Mammalian expression of hArl8 with C-term Myc tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL8A (ARL8A Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDSHA-ARF5-Myc

Plasmid

#67351

Purpose

Mammalian expression of hArf5 with C-term Myc tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF5 (ARF5 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pMALp2x-SCO5461(43-204)

Plasmid

#42509

Depositor

Koji Okamoto

Article

Nakano et al Toxicon. 2012 Dec 2;63C:55-63. doi: 10.1

Insert

guanosine ADP-ribosyltransferase (SCO5461 Streptomyces coelicolor A3(2))

Tags

maltose-binding protein

Expression

Bacterial

Mutation

deleted amino acids 1-42 (nucleotide 1-126)

Promoter

tac

Available Since

April 10, 2013

Availability

Academic Institutions and Nonprofits only
pDONR221-ARF6

Plasmid

#67314

Purpose

hArf6 with stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF6 (ARF6 Human)

Use

Gateway entry vector

Expression

Bacterial

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
CBP-Bromo-HAT-CH3-sp65

Plasmid

#21026

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol. 2001 Nov;21(21):7509-22

Insert

CBP (Crebbp Mouse)

Use

In vitro tnt

Tags

Flag

Mutation

Bromo+HAT+CH3 domains aa 1098-1877; E1239G mutati…

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
gh178

Plasmid

#106828

Purpose

expression of gRNA targeting GYS1

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

GYS1 (GYS1 Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pQCXIpuro-H3.3K4M-FLAG/HA

Plasmid

#128742

Purpose

Expression of histone H3.3K4M-FLAG/HA

Depositor

Kai Ge

Article

Jang et al Nucleic Acids Res. 2019 Jan 25;47(2):607

Insert

H3.3K4M (H3-3A Human)

Use

Retroviral

Tags

FLAG and HA

Mutation

K4M

Promoter

CMV

Available Since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST17-6xHis-ARL2

Plasmid

#67474

Purpose

Expression of hArl2 with N-term His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL2 (ARL2 Human)

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
GFP-PRMT9 L182A/D183A/I184A/G185A

Plasmid

#79676

Purpose

mammalian expression of GFP-PRMT9 catalytically inactive LDIG(182-185)AAAA mutant

Depositor

Steven Clarke

Article

Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

Insert

PRMT9 (PRMT9 Human)

Tags

EGFP

Expression

Mammalian

Mutation

L182A/D183A/I184A/G185A catalytic mutant

Promoter

CMV

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
pDONR221-ARF3

Plasmid

#67308

Purpose

hArf3 with stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF3 (ARF3 Human)

Use

Gateway entry vector

Expression

Bacterial

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only