We narrowed to 79,697 results for: ELL

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pAAV-flex-taCasp3-TEVp

Plasmid

#45580

Depositor

Nirao Shah, Jim Wells

Article

Yang et al Cell. 2013 May 9;153(4):896-909. doi: 10

Has Service

AAV1, AAV5, and AAV8

Inserts

Caspase 3 (CASP3 Synthetic, Human)
Tobacco Etch Virus protease

Use

AAV; Adeno associated viral vector

Mutation

Linker replaced with a TEV protease cleavage site

Promoter

EF1a

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pTRIP-hPGK-Blast-2A-STING-mNeonGreen

Plasmid

#227186

Purpose

Lentiviral expression plasmid encoding STING-mNeonGreen under hPGK promoter

Depositor

Nir Hacohen

Article

Gentili et al Cell Syst. 2024 Dec 18;15(12):1264-1277.

Insert

STING1 (STING1 Human)

Use

Lentiviral

Tags

mNeonGreen

Expression

Mammalian

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-iCreV

Plasmid

#140135

Purpose

Can be used to generate AAV virus that will express light-inducible site-specific iCreV recombinase

Depositor

Hongkui Zeng

Article

Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

Has Service

AAV PHP.eB and AAV1

Insert

iCreV

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EF1alpha

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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AP1muA-TurboID-polyA-G418 HR

Plasmid

#227734

Purpose

Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with TurboID and a V5 epitope tag. Contains a G418 resistance cassette for selection of edited cells.

Depositor

Francesca Bottanelli

Article

Stockhammer et al J Cell Sci. 2024 Aug 15;137(16):jcs26195

Insert

genomic sequence AP1M1 locus (AP1M1 Human)

Use

CRISPR

Tags

Turbo-ID, V5

Available Since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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AAVS1-TRE3G-NFIA+SOX9

Plasmid

#129455

Purpose

Inducible overexpression of NFIA and Sox9 in human cells.

Depositor

Su-Chun Zhang

Article

Li et al Stem Cell Reports. 2018 Oct 9;11(4):998-

Insert

Sox 9 (SOX9 Human)

Expression

Mammalian

Promoter

TRE3G

Available Since

March 5, 2020

Availability

Academic Institutions and Nonprofits only

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PCDH-WT-ALKBH5-3xFLAG

Plasmid

#203736

Purpose

Overexpression of ALKBH5-WT

Depositor

Jianjun Chen

Article

Shen et al Cell Stem Cell. 2020 Jul 2;27(1):64-80.e

Insert

ALKBH5 (ALKBH5 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Available Since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV_msfGFP-SEPT7

Plasmid

#180315

Purpose

mammalian expression of human SEPT7 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN7 (SEPTIN7 Human)

Tags

msfGFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pBABE-hygro-2xMyc-Rac1-P29S

Plasmid

#128581

Purpose

For mammalian cell expression of P29S mutant murine RAC1 carrying 2x Myc epitope tag sequences at the N-terminus.

Depositor

Julian Downward

Article

Lionarons et al Cancer Cell. 2019 Jul 8;36(1):68-83.e9.

Insert

Rac1 (Rac1 Mouse)

Use

Retroviral

Tags

2xMyc epitope

Expression

Mammalian

Mutation

Changed Proline29 to Serine.

Available Since

Aug. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pBABE-hygro-2xMyc-Rac1-Q61L

Plasmid

#128582

Purpose

For mammalian cell expression of Q61L mutant murine RAC1 carrying 2x Myc epitope tag sequences at the N-terminus.

Depositor

Julian Downward

Article

Lionarons et al Cancer Cell. 2019 Jul 8;36(1):68-83.e9.

Insert

Rac1 (Rac1 Mouse)

Use

Retroviral

Tags

2xMyc epitope

Expression

Mammalian

Mutation

Changed Glutamine61 to Leucine.

Available Since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-iDreV

Plasmid

#140136

Purpose

can be used to generate AAV virus that will express light-inducible site-specific iDreV recombinase

Depositor

Hongkui Zeng

Article

Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

Has Service

AAV PHP.eB and AAV1

Insert

iDreV

Use

AAV

Promoter

EF1a

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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TGFbetaR1 (T204D)-pcw107-V5

Plasmid

#64629

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

TGFBR1 (transcript variant 1) (TGFBR1 Human)

Use

Lentiviral

Tags

V5

Mutation

T204D

Promoter

PGK

Available Since

June 1, 2015

Availability

Academic Institutions and Nonprofits only
pUC19-OCT4-T2A-NLS-EmGFP-P2A-Puro

Plasmid

#89992

Purpose

Donor template for generation of OCT4-T2A-nEmGFP reporter cell lines

Depositor

Timo Otonkoski

Article

Balboa et al Stem Cell Res. 2017 Aug;23:105-108. doi:

Insert

OCT4-T2A-NLS-EmGFP-P2A-Puro (POU5F1 Human)

Use

CRISPR

Promoter

none

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT2-msfGFP

Plasmid

#180318

Purpose

mammalian expression of human SEPT2 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN2 (SEPTIN2 Human)

Tags

msfGFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS-PHFF-sh-hBVRA

Plasmid

#100285

Purpose

Expression vector for phycocyanobilin (PCB) synthesis and shRNA for human BVRA gene in mammalian cells

Depositor

Kazuhiro Aoki

Article

Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

Inserts

MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
shRNA for human BVRA

Tags

FLAG, T7 and Mitochondria-targeting sequence (MTS…

Expression

Mammalian

Mutation

All genes are codon-optimized for expression in h…

Promoter

CAG promoter and H1 promoter

Available Since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only

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FLT1-KDR

Plasmid

#86034

Purpose

chimera of human VEGFR1 extracellular domain with the TMD fused to the intracellular domain of VEGFR2

Depositor

Christie Thomas

Article

Raikwar et al Exp Cell Res. 2016 May 15;344(1):103-11.

Insert

human VEGFR1-VEGFR2 hybrid (FLT1, KDR Human)

Tags

HA and t-GFP

Expression

Mammalian

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i3-mApple

Plasmid

#180323

Purpose

mammalian expression of human SEPT9_i3 fused to monomeric Apple

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN9_v3 (SEPTIN9 Human)

Tags

mApple

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-RNF2-ngRNA+41_EF1a-puroR

Plasmid

#207358

Purpose

Lentiviral transfer plasmid encoding hU6-driven expression of a RNF2 nicking guide RNA and EF1a-driven puromycin resistance gene.

Depositor

Marianne Carlon

Article

Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

Insert

RNF2 +41 ngRNA

Use

Lentiviral

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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C1ORF43-3xFLAG

Plasmid

#130880

Purpose

Expresses human C1ORF43 with C-terminal 3xFLAG tag

Depositor

Michael Bassik

Article

Jeng et al Cell Host Microbe. 2019 Oct 9;26(4):551-

Insert

C1ORF43 (C1orf43 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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C1ORF43(1-41)-3xFLAG

Plasmid

#130881

Purpose

Expresses first 41 amino acids of human C1ORF43 with C-terminal 3xFLAG tag

Depositor

Michael Bassik

Article

Jeng et al Cell Host Microbe. 2019 Oct 9;26(4):551-

Insert

C1ORF43 (C1orf43 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Deleted amino acids 44-253

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-CCreV

Plasmid

#140132

Purpose

can be used to generate AAV virus that will express fusion protein of split Cre (C-terminal) and fungal photoreceptor Vivid (VVD) monomer

Depositor

Hongkui Zeng

Article

Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

Insert

CCreV

Use

AAV

Promoter

EF1a

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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