Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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pDONR221_SLC31A1

Plasmid

#132252

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC31A1 (SLC31A1 Human)

Expression

Mammalian

Available Since

Nov. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pCR8/GW/TOPO-ETV1

Plasmid

#98623

Purpose

Gateway entry TOPO TA cloning vector for ETV1

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

ETV1 (Etv1 Mouse)

Use

Topo ta cloning vector

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR221-APP751

Plasmid

#193772

Purpose

Entry vector for human APP isoform having 751 amino acid residues

Depositor

Wade Harper

Article

Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

Insert

APP (APP Human)

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC22A11

Plasmid

#131963

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC22A11 (SLC22A11 Human)

Expression

Mammalian

Available Since

Oct. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC44A5

Plasmid

#132155

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC44A5 (SLC44A5 Human)

Expression

Mammalian

Available Since

Oct. 22, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC26A11_STOP

Plasmid

#161162

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC26A11 (SLC26A11 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SPNS3

Plasmid

#132285

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SPNS3 (SPNS3 Human)

Expression

Mammalian

Available Since

Dec. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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HTR1D-DuET

Plasmid

#213318

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

HTR1D (HTR1D Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLCO4A1

Plasmid

#132236

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLCO4A1 (SLCO4A1 Human)

Expression

Mammalian

Available Since

Nov. 8, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC25A1

Plasmid

#132299

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC25A1 (SLC25A1 Human)

Expression

Mammalian

Available Since

Nov. 12, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pCR8/GW/TOPO-ELK4

Plasmid

#98617

Purpose

Gateway entry TOPO TA cloning vector for ELK4

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

ELK4 (Elk4 Mouse)

Use

Topo ta cloning vector

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC10A2

Plasmid

#131919

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC10A2 (SLC10A2 Human)

Expression

Mammalian

Available Since

Oct. 15, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC4A10

Plasmid

#132308

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC4A10 (SLC4A10 Human)

Expression

Mammalian

Available Since

Nov. 8, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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p5E-MCS-c-Fos-b-globin (JDW 1237)

Plasmid

#229829

Purpose

A gateway compatible 5' entry clone containing an MCS upstream of the murine c-fos minimal promotor and b-globin intron.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

c-fos minimal promoter and b-globin intron

Use

Gateway Cloning

Expression

Mammalian

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC41A2

Plasmid

#132154

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC41A2 (SLC41A2 Human)

Expression

Mammalian

Available Since

Oct. 22, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC43A3

Plasmid

#132202

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC43A3 (SLC43A3 Human)

Expression

Mammalian

Available Since

Oct. 28, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC2A6

Plasmid

#132078

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC2A6 (SLC2A6 Human)

Expression

Mammalian

Available Since

Nov. 11, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC22A16

Plasmid

#132023

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC22A16 (SLC22A16 Human)

Expression

Mammalian

Available Since

Oct. 25, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC12A7

Plasmid

#131873

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC12A7 (SLC12A7 Human)

Expression

Mammalian

Available Since

Oct. 9, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR221 UBR5 ∆PABC

Plasmid

#81064

Purpose

Gateway entry vector encoding full length UBR5 Y2402A/K2415A (∆PABC)

Depositor

Darren Saunders

Article

Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

Insert

Ubiquitin protein ligase E3 component N-recognin 5 (UBR5) ∆PABC (UBR5 Human)

Use

Cloning

Mutation

Y2402A and K2415A (bp t7204g, a7205c, t7206c, a72…

Promoter

N/A

Available Since

Jan. 3, 2017

Availability

Academic Institutions and Nonprofits only

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