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Grx1-roGFP2-Dcp1b

Plasmid

#236547

Purpose

Expressing fluorescent Grx1-roGFP2 fusion protein in mammalian cells with localization in processing bodies.

Depositor

Pablo Rivera-Fuentes

Insert

Grx1-roGFP2-Dcp1b (DCP1B Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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CasRx gRNA targeting EZH2

Plasmid

#219655

Purpose

hU6-driven expression of CasRx guide RNA targeting EZH2. 5' processed DR followed by EZH2 guide.

Depositor

Weixin Tang

Article

Yan et al Nat Chem Biol. 2024 Jul 5. doi: 10.1038/

Insert

CasRx gRNA targeting EZH2

Use

CRISPR

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Trex2

Plasmid

#79246

Purpose

Expression vector for Trex2, which is a non-processive 3' exonuclease.

Depositor

Jeremy Stark

Article

Bennardo et al PLoS Genet. 2009 Oct;5(10):e1000683. doi

Insert

Trex2 (Trex2 Mouse)

Expression

Mammalian

Promoter

pCAGGS

Available Since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCF711

Plasmid

#121662

Purpose

U6-sgRNA EFS-ProCas9-FlaviS6-P2A-Puro

Depositor

David Savage

Article

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Inserts

Use

Lentiviral

Promoter

U6

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCF713

Plasmid

#121665

Purpose

U6-sgRNA EF1a-ProCas9-FlaviS6-P2A-Puro

Depositor

David Savage

Article

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Inserts

Use

Lentiviral

Promoter

U6

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAN102

Plasmid

#220045

Purpose

Mammalian expression of type III-A crRNA and Cas6 crRNA-processing ribonuclease from Streptococcus thermophilus

Depositor

Blake Wiedenheft

Article

Nemudraia et al Science. 2024 Apr 25:eadk5518. doi: 10.1

Insert

crRNA; Cas6

Expression

Mammalian

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMBP-LbCas12a H759A

Plasmid

#113518

Purpose

Expresses His-MBP fused to LbCas12a with H759A mutation to inactivate crRNA processing

Depositor

Jennifer Doudna

Article

Chen et al Science. 2018 Apr 27;360(6387):436-439.

Insert

LbCas12a

Tags

MBP

Expression

Bacterial

Mutation

H759A

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pCF730

Plasmid

#121668

Purpose

EF1a-ProCas9-Flavi-P2A-Puro

Depositor

David Savage

Article

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Insert

Cas9(C)

Use

Lentiviral

Promoter

EF-1a core promoter

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pQE30-AtAEP2

Plasmid

#159529

Purpose

Expresses Arabidopsis thaliana asparaginyl endopeptidase 2 in E. coli

Depositor

Joshua Mylne

Article

Bernath-Levin et al Chem Biol. 2015 May 21;22(5):571-82. doi

Insert

Arabidopsis thaliana asparaginyl endopeptidase 2

Tags

Six-Histidine tag

Expression

Bacterial

Promoter

T5

Available Since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ141-ZmUbi-RZ-Bh

Plasmid

#136378

Purpose

Gateway entry clone for BhCas12b sgRNA expression under ZmUbi promoter with ribozyme processing

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pCF704

Plasmid

#121658

Purpose

U6-sgRNA EFS-ProCas9-Flavi-P2A-Puro

Depositor

David Savage

Article

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Insert

Cas9(C)

Use

Lentiviral

Promoter

U6

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCF712

Plasmid

#121663

Purpose

U6-sgRNA EF1a-ProCas9-Flavi-P2A-Puro

Depositor

David Savage

Article

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Insert

Cas9(C)

Use

Lentiviral

Promoter

U6

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-N-HA_NEDD8

Plasmid

#221859

Purpose

Expresses WT unprocessed NEDD8 with a HA tag in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

NEDD8 (NEDD8 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pXR003: CasRx gRNA cloning backbone

Plasmid

#109053

Purpose

hU6-driven expression of guide RNAs compatible with CasRx. 5' processed DR followed by BbsI sites for guide cloning.

Depositor

Patrick Hsu

Article

Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pIS003

Plasmid

#194014

Purpose

This plasmid is designed for use in a procedure to synthesize single-stranded DNA containing only the insert.

Depositor

Timothy Whitehead

Article

Strawn et al Biotechnol Bioeng. 2023 Oct;120(10):3057

Insert

C16 Ex4 XTR f1

Expression

Bacterial

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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plk1 WT GFP-myc

Plasmid

#132963

Purpose

To study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damage

Depositor

Michael Yaffe

Article

Kishi et al Mol Cell Biol. 2009 Jun;29(11):3134-50.

Insert

polo-like kinase 1 (PLK1 Human)

Expression

Mammalian

Available Since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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plk1 T210D

Plasmid

#132964

Purpose

To study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damage

Depositor

Michael Yaffe

Article

Kishi et al Mol Cell Biol. 2009 Jun;29(11):3134-50.

Insert

polo-like kinase 1 (PLK1 Human)

Expression

Mammalian

Mutation

T210D

Available Since

Feb. 17, 2023

Availability

Academic Institutions and Nonprofits only

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plk1 T210A

Plasmid

#132965

Purpose

To study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damage

Depositor

Michael Yaffe

Article

Kishi et al Mol Cell Biol. 2009 Jun;29(11):3134-50.

Insert

polo-like kinase 1 T210A (PLK1 Human)

Expression

Mammalian

Mutation

T210A

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsRRP12_K

Plasmid

#146766

Purpose

Mammalian Expression of HsRRP12

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsRRP12 (RRP12 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pIS002

Plasmid

#194013

Purpose

This plasmid is designed for use in a procedure to synthesize single-stranded DNA containing only the insert.

Depositor

Timothy Whitehead

Article

Strawn et al Biotechnol Bioeng. 2023 Oct;120(10):3057

Insert

C16 Ex3 XTR f1

Expression

Bacterial

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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