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Plasmid#50590PurposeCRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sitesDepositorInsertT1, U626t plus, U629p, T2
UseCRISPR; Pcr templateTagsExpressionPlantMutationPromoterAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
p5E-hPGK
Plasmid#82579Purpose5' entry vector containing human phosphoglycerate kinase (hPGK) promoter for ubiquitous expressionDepositorInsertHuman PGK promoter
UseTagsExpressionMutationPromoterNoneAvailable sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-MAT2A
Plasmid#100521PurposeDox-repressible cDNA expression of MAT2A (dox-off/ tet-off all-in-one vector)DepositorInsertMAT2A (MAT2A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterpTight TREAvailable sinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
FH-TET3-pEF
Plasmid#49446PurposeThis plasmid expresses full-length human TET3.DepositorInsertTet methylcytosine dioxygenase 3 (TET3 Human)
UseTagsFlag and HAExpressionMammalianMutationPromoterEF-1alphaAvailable sinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
TFORF0987
Plasmid#142715PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertKLF2 (KLF2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-EFS-BirAopt-GSQ-RBXN-P2A-BLAST
Plasmid#208048PurposeEnables constitutive expression of BirAopt alone; RBXN represents a EcoR1-BsiW1-Xba1-Not1 polylinker; to perform bioE3; selection with blasticidinDepositorInsertBirAopt
UseLentiviralTagsExpressionMutationBirAopt is human-codon-optimized BirA (PMID 15707…PromoterEFSAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLX303-ABI1-dCas9
Plasmid#154474PurposeExpresses ABI1 fused to the N terminus of dCas9DepositorInsertABI1 (ABI1 Mustard Weed)
UseLentiviralTagsExpressionMammalianMutationIncludes ABI1 aa 126-423, D143APromoterCMVAvailable sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
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EB3-GFP
Plasmid#190164PurposeExpresses EB3-GFP in mammalian cellsDepositorInsertEB3-GFP
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-NbALFA
Plasmid#159986PurposeFor the expresson and purification of the EGFP-tagged anti-ALFA tag nanobody (NbALFA)DepositorInsertmEGFP-NbALFA
UseTags8histidine-HRV3C protease cleavage siteExpressionBacterialMutation3GS linker between mEGFP and NbALFAPromoterAvailable sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only