We narrowed to 11,282 results for: Vars;

1. F. prausnitzii 16S toehold switch sensor

   Plasmid

   #110705

   Purpose

   Toehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output

   Depositor

   James Collins

   Article

   Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

   Insert

   F. prausnitzii 16S toehold switch sensor
   

   Use

   Synthetic Biology

   Promoter

   T7

   Available Since

   Sept. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. R. hominis 16S toehold switch sensor

   Plasmid

   #110703

   Purpose

   Toehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output

   Depositor

   James Collins

   Article

   Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

   Insert

   R. hominis 16S toehold switch sensor
   

   Use

   Synthetic Biology

   Promoter

   T7

   Available Since

   Sept. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. E. rectale 16S toehold switch sensor

   Plasmid

   #110702

   Purpose

   Toehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output

   Depositor

   James Collins

   Article

   Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

   Insert

   E. rectale 16S toehold switch sensor
   

   Use

   Synthetic Biology

   Promoter

   T7

   Available Since

   Sept. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. B. breve 16S toehold switch sensor

   Plasmid

   #110701

   Purpose

   Toehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output

   Depositor

   James Collins

   Article

   Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

   Insert

   B. breve 16S toehold switch sensor
   

   Use

   Synthetic Biology

   Promoter

   T7

   Available Since

   Sept. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. B. adolescentis 16S toehold switch sensor

   Plasmid

   #110700

   Purpose

   Toehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output

   Depositor

   James Collins

   Article

   Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

   Insert

   B. adolescentis 16S toehold switch sensor
   

   Use

   Synthetic Biology

   Promoter

   T7

   Available Since

   Sept. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. B. longum 16S toehold switch sensor

   Plasmid

   #110699

   Purpose

   Toehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output

   Depositor

   James Collins

   Article

   Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

   Insert

   B. longum 16S toehold switch sensor
   

   Use

   Synthetic Biology

   Promoter

   T7

   Available Since

   Sept. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. B. thetaiotaomicron 16S toehold switch sensor

   Plasmid

   #110697

   Purpose

   Toehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output

   Depositor

   James Collins

   Article

   Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

   Insert

   B. thetaiotaomicron 16S toehold switch sensor
   

   Use

   Synthetic Biology

   Promoter

   T7

   Available Since

   Sept. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. SCRIB_PDZ_2

   Plasmid

   #103884

   Purpose

   Protein expression and purification of Scribble PDZ2 domain

   Depositor

   Sachdev Sidhu

   Article

   Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

   Insert

   SCRIB (SCRIB Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. HA-mus-Bnip3_deltaExon3

   Plasmid

   #100793

   Purpose

   Mammalian expression of Bnip3_deltaExon3 splice variant

   Depositor

   Joseph Gordon

   Article

   Diehl-Jones et al J Pediatr Gastroenterol Nutr. 2015 Nov;6

   Insert

   mouse-Bnip3_deltaExon3 (Bnip3 Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Exon3 deletion

   Promoter

   CMV

   Available Since

   Sept. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGS-3R112C/T180C

    Plasmid

    #85095

    Purpose

    Host vector to fuse a gene at the N-terminus of PCNA3 variant (R112C/T180C)

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

    Insert

    DNA polymerase sliding clamp 3
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    changed Arginine 112 to Cysteine and Threonine 18…

    Promoter

    T7 promoter

    Available Since

    Nov. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. MSCV-6NBC-GFP-PGK-Puro-Barcode81

    Plasmid

    #83758

    Purpose

    individual 6N barcode to make barcoded cell line variants

    Depositor

    Monte Winslow

    Article

    Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

    Insert

    Barcode81
    

    Use

    Retroviral

    Mutation

    deletion of T in starting ATG of GFP (please see …

    Available Since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. MSCV-6NBC-GFP-PGK-Puro-Barcode71

    Plasmid

    #83748

    Purpose

    individual 6N barcode to make barcoded cell line variants

    Depositor

    Monte Winslow

    Article

    Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

    Insert

    Barcode71
    

    Use

    Retroviral

    Mutation

    deletion of AA5-233 in GFP (please see depositor …

    Available Since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSB700 lenti gRNA Cerulean-zhang2.0-Weiss-chimera2

    Plasmid

    #79380

    Purpose

    Cas9 gRNA variant to recruit ms2 proteins

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    Chimera 2
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. TALEN-CCR5L18-NtermN3

    Plasmid

    #51448

    Purpose

    Expresses TALEN targeting left CCR5A site (L18) with N3 N-terminal domain

    Depositor

    David Liu

    Article

    Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

    Insert

    TALEN CCR5 Left (L18) N3 N-term
    

    Use

    TALEN

    Tags

    3xFLAG and Fok1 EL variant

    Expression

    Mammalian

    Mutation

    K150Q, K153Q, and R154Q

    Promoter

    CMV

    Available Since

    May 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. TALEN-CCR5R18-NtermN3

    Plasmid

    #51449

    Purpose

    Expresses TALEN targeting right CCR5A site (R18) with N3 N-terminal domain

    Depositor

    David Liu

    Article

    Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

    Insert

    TALEN CCR5 Right (R18) N3 N-term
    

    Use

    TALEN

    Tags

    3xFLAG and Fok1 KK variant

    Expression

    Mammalian

    Mutation

    K150Q, K153Q, and R154Q

    Promoter

    CMV

    Available Since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. refGFP1_pQE30

    Plasmid

    #48644

    Purpose

    Bact exp vector encoding for the 6XHis tagged insensitive variant of the fluorescent redox probe roGFP with mut Cys147 to Serine, del insertion147b and mutated Cys204 to Glut.Referred to as refGFP

    Depositor

    David Ron

    Article

    Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.

    Insert

    refGFP1
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    refGFP C147S, C204Q

    Available Since

    Oct. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSB700 lenti gRNA Cerulean-zhang2.0-Weiss-chimera1

    Plasmid

    #79379

    Purpose

    Cas9 gRNA variant to recruit ms2 proteins

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    Chimera 1
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSB700 lenti gRNA Cerulean-zhang2.0-Weiss-chimera4

    Plasmid

    #79382

    Purpose

    Cas9 gRNA variant to recruit ms2 proteins

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    Chimera 4
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET41a iGlum

    Plasmid

    #119829

    Purpose

    Production of recombinant iGluSnFR R24K medium affinity variant, a single-wavelength glutamate indicator, in E. coli

    Depositor

    Katalin Torok

    Article

    Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

    Insert

    iGlum
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    R24K

    Promoter

    T7

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAF0033 Ef1a-Pa01-2a-EGFP

    Plasmid

    #193460

    Purpose

    Expression of Pa01 recombinase from Ef1a promoter

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Pa01
    

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAF0036 Ef1a-Si74-2a-EGFP

    Plasmid

    #193463

    Purpose

    Expression of Si74 recombinase from Ef1a promoter

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Si74
    

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pTH727-CEN-RLuc/staCFLuc

    Plasmid

    #38211

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Inserts

    Firefly Luciferase
    Renilla luciferase
    

    Expression

    Yeast

    Mutation

    The last three codons of the full-length Firefly …

    Promoter

    ADH1 and TDH3 (=GPD)

    Available Since

    Oct. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAF0035 Ef1a-Nm60-2a-EGFP

    Plasmid

    #193464

    Purpose

    Expression of Nm60 recombinase from Ef1a promoter

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Nm60
    

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAF0084 Ef1a-Cp36-2a-EGFP

    Plasmid

    #193462

    Purpose

    Expression of Cp36 recombinase from Ef1a promoter

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Cp36
    

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAF0034 Ef1a-Kp03-2a-EGFP

    Plasmid

    #193461

    Purpose

    Expression of Kp03 recombinase from Ef1a promoter

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Kp03
    

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV.Syn.GCaMP6f.WPRE.SV40

    Plasmid

    #100837

    Purpose

    AAV expression of ultrasensitive protein calcium sensor from Synapsin promoter

    Depositor

    Douglas Kim, GENIE Project

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Has Service

    AAV1, AAV5, and AAV9

    Insert

    GCaMP6f
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    GCaMP3-T302L R303P A317E D380Y T381R S383T R392G

    Promoter

    Synapsin

    Available Since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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27. pDACR3883 [rab-3p::HYlight-RA::let-858utr]

    Plasmid

    #207667

    Purpose

    Expresses HYlight-RA, the negative control HYlight variant with reduced binding affinity, in C. elegans neurons

    Depositor

    Daniel Colón-Ramos

    Article

    Wolfe et al Proc Natl Acad Sci U S A. 2024 Jan 16;12

    Insert

    HYlight-RA [T152E]
    

    Expression

    Worm

    Promoter

    rab-3

    Available Since

    Feb. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCasTet-λ

    Plasmid

    #128318

    Purpose

    Constitutive expression of cas9 and anhydrotetracycline/tetracycline inducible expression of lamda RED. Useful variant of Plasmid #62225 when arabinose induction is not possible.

    Depositor

    Christopher Johnston

    Article

    Johnston et al Proc Natl Acad Sci U S A. 2019 May 16. p

    Insert

    TetR and pTetR/TetO
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Mutation

    Constitutive expression of cas9 and anhydro-tetra…

    Promoter

    tet promoter

    Available Since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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29. pAAV.CAG.GCaMP6s.WPRE.SV40

    Plasmid

    #100844

    Purpose

    AAV expression of ultrasensitive protein calcium sensor from CAG promoter

    Depositor

    Douglas Kim, GENIE Project

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Has Service

    AAV1, AAV5, and AAV9

    Insert

    GCaMP6s
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    GCaMP3-K78H T302L R303P D380Y T381R S383T R392G

    Promoter

    CAG

    Available Since

    Feb. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGP-pcDNA3.1 Puro-CAG-Positron2

    Plasmid

    #239079

    Purpose

    Mammalian expression of positive-going voltage sensor

    Depositor

    GENIE Project

    Article

    Eric R. et al Janelia Figshare 2023

    Insert

    Positron2
    

    Expression

    Mammalian

    Mutation

    R78K N81D D92N W178F

    Promoter

    CAG

    Available Since

    July 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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