We narrowed to 6,905 results for: crispr cas9 plasmids

1. EED-KO-gRNA-2

   Plasmid

   #131322

   Purpose

   gRNA to knockout EED. Use with EED-KO-gRNA-1

   Depositor

   Danny Reinberg

   Article

   Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

   Insert

   EED-KO-gRNA-2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

2. gRNA-HEK3-Puro

   Plasmid

   #136282

   Purpose

   Expresses a guide RNA targeting HEK3 site

   Depositor

   Zhaohui Ye

   Article

   McGrath et al Nat Commun. 2019 Nov 25;10(1):5353. doi:

   Insert

   sgRNA-HEK3
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. 5xtetO (tet3) Gene Desert

   Plasmid

   #131339

   Purpose

   gRNA to insert 5xTet operator sequence into a gene desert on chromosome 5.

   Depositor

   Danny Reinberg

   Article

   Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

   Insert

   Desert-gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. 5xtetO HoxC 3'

   Plasmid

   #131340

   Purpose

   gRNA to insert 5xTet operator sequence into downstream of HoxC cluster.

   Depositor

   Danny Reinberg

   Article

   Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

   Insert

   HoxC-downstream-gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. EZH2-Promoter-gRNA

   Plasmid

   #131329

   Purpose

   gRNA to generate endogenously tagged -tetR-EZH2

   Depositor

   Danny Reinberg

   Article

   Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

   Insert

   EZH2-Promoter-gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. EED-F97A

   Plasmid

   #131326

   Purpose

   gRNA to generate F97A cage-mutant EED

   Depositor

   Danny Reinberg

   Article

   Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

   Insert

   EED-KO-gRNA-2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. peSCBE3-NG-HF1-Hypa

   Plasmid

   #218166

   Purpose

   This plasmid harbors the base editor eSCBE3-NG-HF1-Hypa along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity base editing at targets bearing NG PAM in Streptomyces.

   Depositor

   Yuhui Sun

   Article

   Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

   Inserts

   escbe3-NG-HF1-Hypa
   a programmable sgRNA cloning cassette
   

   Use

   CRISPR; Sgrna transcription

   Tags

   Expression

   Bacterial

   Mutation

   Mutation in the portion of deaminase hAPOBEC3A : …

   Promoter

   rpsL promoter

   Available since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX459-sgRNA2-CTCF-prom

   Plasmid

   #195104

   Purpose

   Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting upstream of the human CTCF promoter. Puromycin selection (2A-fusion).

   Depositor

   Job Dekker

   Article

   Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

   Insert

   sgRNA against CTCF promoter
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX459-sgRNA1-CTCF-prom

   Plasmid

   #195103

   Purpose

   Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting upstream of the human CTCF promoter. Puromycin selection (2A-fusion).

   Depositor

   Job Dekker

   Article

   Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

   Insert

   sgRNA against CTCF promoter
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. GG-dest

    Plasmid

    #69538

    Purpose

    Destination plasmid for gRNA concatenation Golden Gate cloning

    Depositor

    Timo Otonkoski

    Article

    Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. scFv-GCN4-DNMT3a-DNMT3l

    Plasmid

    #154140

    Purpose

    Expresses the scFv-GCN4-DNMT3a-DNMT3l fusion protein (more details are shown in the vectro map) for targeted DNA methylation. Should be used in a combination with the dCas9-SunTag systems.

    Depositor

    Albert Jeltsch

    Article

    Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

    Insert

    scFv-GCN4, DNMT3a (catalytic domain), DNMT3l (C-terminal part), sfGFP
    

    Use

    Tags

    HA and sfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Oct. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. PE_CO-Mini-C

    Plasmid

    #190337

    Purpose

    Expressing the C-terminal of PE_CO-Mini using Rma intein and Cas9 673-674 split site, and pegRNA for PCSK9 +1A insertion

    Depositor

    Rasmus Bak

    Article

    Gao et al Mol Ther. 2022 Jul 8. pii: S1525-0016(22

    Insert

    PE_CO-Mini-C, PCSK9 +1A insertion pegRNA
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. PE_CO-Mini-N

    Plasmid

    #190336

    Purpose

    Expressing the N-terminal of PE_CO-Mini using Rma intein and Cas9 673-674 split site, ngRNA and pegRNA for PCSK9 +1A insertion

    Depositor

    Rasmus Bak

    Article

    Gao et al Mol Ther. 2022 Jul 8. pii: S1525-0016(22

    Insert

    PE_CO-Mini-N, ngRNA and pegRNA for PCSK9 +1A insertion
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-EFS-SaKKH-spA-miniU6-miniSdd6-MmHPD-T2

    Plasmid

    #204852

    Purpose

    AAV vector for cytosine base editing using miniSdd6 at HPD-T2 target site in mouse

    Depositor

    Caixia Gao

    Article

    Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

    Insert

    miniSdd6-nSaCas9KKH(D10A)-UGI
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    D10A, E782K, N968K, R1015H in SaCas9

    Promoter

    EFS, miniU6

    Available since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. JARID2-KO-gRNA-1

    Plasmid

    #131328

    Purpose

    gRNA to knockout JARID2. Use with JARID2-KO-gRNA-2

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    JARID2-KO-gRNA-1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

16. JARID2-KO-gRNA-2

    Plasmid

    #131327

    Purpose

    gRNA to knockout JARID2. Use with JARID2-KO-gRNA-1

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    JARID2-KO-gRNA-2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

17. Y365A or A365Y inducible rescue

    Plasmid

    #131323

    Purpose

    gRNA to generate i-WT-r or i-MT-r systems

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    Y365A or A365Y inducible rescue
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. MTF2_KO_ex11_gRNA-4

    Plasmid

    #131334

    Purpose

    gRNA to knockout MTF2. This gRNA is also used in Haojie Li et al. Nature 2017. It targets exon 11 of MTF2.

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    MTF2_KO_ex11_gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. LMX1B-gRNA-N-del

    Plasmid

    #131333

    Purpose

    gRNA to delete a nucleation site near Lmx1b. Use with LMX1B-gRNA-C-del

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    LMX1B-gRNA-N-del
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. HoxD del ds1

    Plasmid

    #131338

    Purpose

    gRNA to delete a nucleation site near HoxD. Use with HoxD del us1

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    EVX2-gRNA-C-del
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge