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pET28-hisSumo-KE-Des27.6

Plasmid

#248002

Purpose

Expression of synthetic Kemp eliminase (Des27.6)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.6

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pET28-hisSumo-KE-Des27.11

Plasmid

#248006

Purpose

Expression of synthetic Kemp eliminase (Des27.11)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.11

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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mCerulean-T-SpyTag

Plasmid

#243787

Purpose

Expresses multifunctional construct enabling the YES-gated release of mCerulean from materials in response to TEV treatment. Construct contains a SpyTag for material tethering.

Depositor

Cole DeForest

Article

Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

Insert

mCerulean

Tags

6x His tag

Expression

Bacterial

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLN051

Plasmid

#247314

Purpose

Expression of PopJ under the control of the PodJ promoter (KanR)

Depositor

Wei Zhao

Article

Lu et al mBio. 2023 Apr 25;14(2):e0321822. doi: 1

Insert

PodJ (podJ Caulobacter crescentus)

Expression

Bacterial

Promoter

PPodJ

Available Since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pLN053

Plasmid

#247313

Purpose

Expression of PopZ fused to mCherry and sfGFP under the control of the PopZ promoter (KanR)

Depositor

Wei Zhao

Article

Lu et al mBio. 2023 Apr 25;14(2):e0321822. doi: 1

Insert

PopZ (popZ Caulobacter crescentus)

Tags

mCherry and sfgfp

Expression

Bacterial

Promoter

PPopZ

Available Since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pLN058

Plasmid

#247316

Purpose

Expression of PodJΔIDR under the control of the PodJ promoter (KanR)

Depositor

Wei Zhao

Article

Lu et al mBio. 2023 Apr 25;14(2):e0321822. doi: 1

Insert

PodJΔIDR (podJ Caulobacter crescentus)

Expression

Bacterial

Promoter

PPodJ

Available Since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pLN055

Plasmid

#247315

Purpose

Expression of PodJΔCC4-6 under the control of the PodJ promoter (KanR)

Depositor

Wei Zhao

Article

Lu et al mBio. 2023 Apr 25;14(2):e0321822. doi: 1

Insert

PodJΔCC4-6 (podJ Caulobacter crescentus)

Expression

Bacterial

Promoter

PPodJ

Available Since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pPD0053_pIVTRup_EBFP2

Plasmid

#242544

Purpose

IVT

Depositor

Christoph Bock, Paul Datlinger

Article

Datlinger et al Nature. 2025 Oct;646(8086):963-972. doi:

Insert

EBFP2

Use

Other

Available Since

Nov. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pET28-hisSumo-KE-Des27.7

Plasmid

#248003

Purpose

Expression of synthetic Kemp eliminase (Des27.7)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.7

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pET28-hisSumo-KE-Des27.9

Plasmid

#248004

Purpose

Expression of synthetic Kemp eliminase (Des27.9)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.9

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pET28-hisSumo-KE-Des27.13

Plasmid

#248008

Purpose

Expression of synthetic Kemp eliminase (Des27.13)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.13

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pEV824

Plasmid

#246453

Purpose

The plasmid comprises an indicible transcriptional unit encoding Gal4-VPR

Depositor

Ron Weiss

Article

Caliendo et al Nat Chem Biol. 2024 Sep 12. doi: 10.1038

Insert

Gal4-VPR

Use

Synthetic Biology

Mutation

Mutated Kozak sequence

Promoter

miniYB

Available Since

Nov. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pEV823

Plasmid

#246452

Purpose

The plasmid comprises an indicible transcriptional unit encoding Gal4-VPR

Depositor

Ron Weiss

Article

Caliendo et al Nat Chem Biol. 2024 Sep 12. doi: 10.1038

Insert

Gal4-VPR

Use

Synthetic Biology

Mutation

Mutated Kozak sequence

Promoter

miniYB

Available Since

Nov. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pEV746

Plasmid

#246449

Purpose

The plasmid comprises an indicible transcriptional unit encoding Gal4-VPR

Depositor

Ron Weiss

Article

Caliendo et al Nat Chem Biol. 2024 Sep 12. doi: 10.1038

Insert

Gal4-VPR

Use

Synthetic Biology

Mutation

Mutated Kozak sequence

Promoter

miniYB

Available Since

Nov. 7, 2025

Availability

Academic Institutions and Nonprofits only

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CIRC-V1-Tet-CVB3 IRES-EGFP-BbsI

Plasmid

#246756

Purpose

RNA circularization with CIRC using Tet intron, harbouring an CVB3 IRES-EGFP reporter.Plasmid was linearized with BbsI.

Depositor

Wensheng Wei

Article

Shen et al Nat Commun. 2025 Aug 10;16(1):7376. doi:

Insert

CIRC-V1-Tet-CVB3 IRES-EGFP-BbsI

Use

Synthetic Biology

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pEV745

Plasmid

#246448

Purpose

The plasmid comprises an indicible transcriptional unit encoding Gal4-VPR

Depositor

Ron Weiss

Article

Caliendo et al Nat Chem Biol. 2024 Sep 12. doi: 10.1038

Insert

Gal4-VPR

Use

Synthetic Biology

Mutation

Mutated Kozak sequence

Promoter

miniYB

Available Since

Oct. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pEV785

Plasmid

#246451

Purpose

The plasmid comprises an indicible transcriptional unit encoding Gal4-VPR

Depositor

Ron Weiss

Article

Caliendo et al Nat Chem Biol. 2024 Sep 12. doi: 10.1038

Insert

Gal4-VPR

Use

Synthetic Biology

Mutation

Mutated Kozak sequence

Promoter

miniYB

Available Since

Oct. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pISEre1

Plasmid

#226819

Purpose

Expressing ISEre1 controled by pCas promoter, λ-red recombinase, SacB and ISEre1 guide RNA targeting pMB1 ori

Depositor

Sheng Yang

Article

Zhou et al Biotechnol J. 2025 Mar;20(3):e70005. doi

Insert

ISEre1

Expression

Bacterial

Available Since

Oct. 20, 2025

Availability

Academic Institutions and Nonprofits only

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mGreenLantern-A OR (S AND T)-SpyTag

Plasmid

#243781

Purpose

Expresses multifunctional construct enabling the OR(AND)-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9) OR (sortase eSrtA(4S9) AND TEV) treatment. Contains a SpyTag for material tethering.

Depositor

Cole DeForest

Article

Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

Insert

mGreenLantern

Tags

6x His tag

Expression

Bacterial

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

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mGreenLantern-S OR (A AND T)-SpyTag

Plasmid

#243782

Purpose

Expresses multifunctional construct enabling the OR(AND)-gated release of mGreenLantern from materials in response to sortase eSrtA(4S9) OR (sortase eSrtA(2A9) AND TEV) treatment. Contains a SpyTag for material tethering.

Depositor

Cole DeForest

Article

Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

Insert

mGreenLantern

Tags

6x His tag

Expression

Bacterial

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

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