We narrowed to 13,293 results for: cDNA

1. pcDNA3.2 mir-1-1 reporter dme-mir-125

   Plasmid

   #46667

   Depositor

   David Bartel

   Article

   Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

   Insert

   dme-mir-125
   

   Use

   RNAi

   Tags

   V5 and hsa-mir-1-1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA4-Tet-on-vsv-Mis12-dCEN Incenp

   Plasmid

   #108482

   Purpose

   inducible expression of vsv-Mis12-dCEN-INCENP

   Depositor

   Susanne Lens

   Article

   Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

   Insert

   Mis12-dCEN-INCENP (INCENP Human)
   

   Use

   Tags

   VSV

   Expression

   Mammalian

   Mutation

   CEN box replaced by Mis12

   Promoter

   CMV

   Available Since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1 Flag YFP hNKCC1 HA-ECL3 (NT933)

   Plasmid

   #49064

   Purpose

   Expresses human NKCC1 mutant with HA tag inserted into extracellular loop #3 and N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

   Depositor

   Biff Forbush

   Article

   Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

   Insert

   human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
   

   Use

   Tags

   3x Flag and mVenus

   Expression

   Mammalian

   Mutation

   2xHA epitope in ECL3 inserted at aa460 in hNKCC1 …

   Promoter

   CMV

   Available Since

   Nov. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5-FRT/TO-neo-C11orf51-3xFlag (2765)

   Plasmid

   #39859

   Depositor

   Jonathon Pines

   Article

   Mansfeld et al Nat Cell Biol. 2011 Sep 18;13(10):1234-4

   Insert

   C11orf51 (ANAPC15 Human)
   

   Use

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 31, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA4/TO CD16.7-h-PKD1 FLM (4078-4303)

   Plasmid

   #83465

   Purpose

   Cytoplasmic tail of human PC1 expression cassette with a membrane anchor and PKD1 sequence that corresponds to amino acids 4078 to 4303 of PC1 (98)

   Depositor

   Thomas Weimbs

   Article

   Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

   Insert

   Polycystin-1 (PKD1 Human)
   

   Use

   Tags

   CD16.7

   Expression

   Mammalian

   Mutation

   Amino-acids 4078-4303

   Promoter

   Available Since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. PcDNA-DEST53-CypB-Nucl N-term GFP

   Plasmid

   #36123

   Depositor

   Patrick Van Oostveldt

   Article

   Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

   Insert

   cyclophilin B (PPIB Human, contains CypB AA 33-216)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   A212S

   Promoter

   CMV

   Available Since

   Nov. 1, 2012

   Availability

   Academic Institutions and Nonprofits only

7. Mito-Cyan Nano-lantern(Ca2+)/pcDNA3

   Plasmid

   #71345

   Purpose

   Express Mito-Cyan Nano-lantern-based luminescent Ca2+ indicator in mammalian cel

   Depositor

   Takeharu Nagai

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   hOTC_Cyan Nano-lantern(Ca2+)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

8. PCDNA3-N3FLAG-MYB-TAD(251-327)

   Plasmid

   #105611

   Purpose

   transient express MYB TAD fragment with 3*FLAG tag at N terminal in HEK 293T cells

   Depositor

   Christopher Vakoc

   Article

   Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

   Insert

   TAD fragment of MYB (mouse), amino acids 251-327 (Myb Mouse)
   

   Use

   Tags

   3*FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. 832 pcDNA3 GFP PTEN delta274-342

   Plasmid

   #10928

   Depositor

   William Sellers

   Insert

   PTEN (PTEN Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Deletion of amino acids 274-342

   Promoter

   Available Since

   Jan. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

10. p5064 pcDNA3.1/V5-His-TOPO hBrd4 (1-461)

    Plasmid

    #14443

    Depositor

    Peter Howley

    Article

    You et al Cell. 2004 Apr 30. 117(3):349-60.

    Insert

    Brd4 (1-461) (BRD4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    amino acids 1-461

    Promoter

    Available Since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA5 FRT TO myc Separase delta 55/S1126A

    Plasmid

    #59825

    Purpose

    Allows the integration of myc Separase delta 55/S1126A in the genome and Tet-inducible expression.

    Depositor

    Stephen Taylor

    Article

    Holland et al J Biol Chem. 2007 Aug 24;282(34):24623-3

    Insert

    Separase (ESPL1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    delta 55/S1126A

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available Since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA3.1(-) + SLNCR1 _462-572 (conserved sequence)

    Plasmid

    #86818

    Purpose

    mammalian expression of lncRNA LINC00673/SLNCR1 deletion construct

    Depositor

    Carl Novina

    Article

    Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

    Insert

    LINC00673 (LINC00673 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1(+) Laccase2 MCS-ZKSCAN1 548-1047

    Plasmid

    #69896

    Purpose

    Expresses a miniature version of the ZKSCAN1 circular RNA in mammalian cells

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    ZKSCAN1 (stw, ZKSCAN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1 Flag YFP hNKCC1 A734C (NT508)

    Plasmid

    #50888

    Purpose

    Expresses human NKCC1 A734C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Insert

    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    A734C in hNKCC1 in NT17

    Promoter

    CMV

    Available Since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. YFP(159-238)-gamma-10 in pcDNAI/Amp

    Plasmid

    #55192

    Purpose

    A carboxyl-terminal YFP fragment was fused to Ggamma-10. When co-expressed with an amino-terminal YFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.

    Depositor

    Catherine Berlot

    Article

    Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E

    Insert

    YFP-(159-238)-gamma-10 (GNG10 Aequorea victoria, Human)
    

    Use

    Tags

    YFP(159-238) was fused to the amino terminus of G…

    Expression

    Mammalian

    Mutation

    Ggamma-10 was amplified by PCR, which added a Bam…

    Promoter

    CMV

    Available Since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. YFP(159-238)-gamma-12 in pcDNAI/Amp

    Plasmid

    #55194

    Purpose

    A carboxyl-terminal YFP fragment was fused to Ggamma-12. When co-expressed with an amino-terminal YFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.

    Depositor

    Catherine Berlot

    Article

    Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E

    Insert

    YFP(159-238)-gamma-12 (GNG12 Aequorea victoria, Human)
    

    Use

    Tags

    YFP(159-238) was fused to the amino terminus of G…

    Expression

    Mammalian

    Mutation

    Ggamma-12 was amplified by PCR, which added a Bam…

    Promoter

    CMV

    Available Since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA-dCas9-p300 Core (1396/1397 SY/WW)

    Plasmid

    #61363

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation 1396/1397 SY/WW) driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Insert

    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, Human, S. pyogenes)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9; 1396/1397 SY/WW m…

    Promoter

    CMV

    Available Since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. 3187 pSFFV-neo Bad cDNA ORF

    Plasmid

    #8756

    Depositor

    Stanley Korsmeyer

    Article

    Zha et al Cell 1996 Nov 15;87(4):619-28.

    Insert

    BAD (Bad Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 18, 2006

    Availability

    Academic Institutions and Nonprofits only

19. PcDNA-DEST53-CypB-Del4 N-term GFP

    Plasmid

    #36137

    Depositor

    Patrick Van Oostveldt

    Article

    Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

    Insert

    cyclophilin B (PPIB Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    contains amino acids 42-216 of cyclophilin B

    Promoter

    CMV

    Available Since

    Sept. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

20. NC12 pCDNA3.1 VENUS WTX delta ETGE

    Plasmid

    #36967

    Depositor

    Randall Moon

    Article

    Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50.

    Insert

    WTX (delta ETGE motif) (AMER1 Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    lacks amino acids 288 –291

    Promoter

    CMV

    Available Since

    July 24, 2012

    Availability

    Academic Institutions and Nonprofits only