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Showing: 1321 - 1360 of 1450 results

  1. pILGFP3A2 (1)

    Plasmid
    #185851
    Purpose
    Testing the TEF1 promoter appended with 2 tetracycline riboswitch using a green fluorescence protein as the reporter
    Depositor
    Claudia Vickers
    Article
    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:
    Insert
    PURA3>KlURA3>TAgTEF1-PTEF1>2*TcRb>yEGFP>TPGK1-TURA3
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    185851-plasmid-map-sequence-id-370760

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  4. pGLO-GFP-3UAG

    Plasmid
    #82501
    Purpose
    Expresses GFP with 3 UAG codons at the amino acid position 3, 151, and 153
    Depositor
    Chang Liu
    Article
    Schwessinger et al Integr Biol (Camb). 2016 Apr 18;8(4):542
    Insert
    Green Fluorescence Protein with 3 UAG codons
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Added an UAG codon at the amino acid position 3, …
    Promoter
    AraBAD
    Available since
    Sept. 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    82501-plasmid-map-sequence-id-156568

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  7. env8 selectivity (b')

    Plasmid
    #99837
    Purpose
    mNeonGreen reporter that places expression of a fluorescent protein reporter (mNeonGreen) under control of the env8HyCbl riboswitch.
    Depositor
    Robert Batey
    Article
    Polaski et al J Biol Chem. 2017 May 8. pii: jbc.M117.7
    Insert
    env8 selectivity (b')
    Use
    Tags
    mNeonGreen
    Expression
    Bacterial
    Mutation
    A20G, A68G, G-C pairs in P1 and P3
    Promoter
    Available since
    April 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    99837-plasmid-map-sequence-id-192118

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  23. HSC1-HS4-GiP

    Plasmid
    #58540
    Purpose
    Derived from HSC1-GiP where 2 copies of the HS4 insulator core are cloned inside the 3'LTR
    Depositor
    James Ellis
    Article
    Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1
    Inserts
    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    Core HS4 insulator sequence (dimer)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human EF1-alpha
    Available since
    Sept. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    58540-plasmid-map-sequence-id-90629

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  28. HSC1-D4Z4-GiP

    Plasmid
    #58542
    Purpose
    Derived from HSC1-GiP where the D4Z4 insulator is cloned upstream of the EF1 alpha promoter
    Depositor
    James Ellis
    Article
    Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1
    Inserts
    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    D4Z4 repeat, partial
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human EF1-alpha
    Available since
    Sept. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    58542-plasmid-map-sequence-id-90651

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  35. env8 selectivity (d')

    Plasmid
    #99838
    Purpose
    mNeonGreen reporter that places expression of a fluorescent protein reporter (mNeonGreen) under control of the env8HyCbl riboswitch.
    Depositor
    Robert Batey
    Article
    Polaski et al J Biol Chem. 2017 May 8. pii: jbc.M117.7
    Insert
    env8 selectivity (d')
    Use
    Tags
    mNeonGreen
    Expression
    Bacterial
    Mutation
    A20G, A68G, G-C pairs in P1 and P3, G12A, G10U de…
    Promoter
    Available since
    April 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    99838-plasmid-map-sequence-id-192115

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  36. pH6-2xmNeonC

    Plasmid
    #178463
    Purpose
    A tandem mNeonGreen fluorescent protein (2xmNeon) with an N-terminal 6xHis-tag and C-terminal cysteine in pET28a.
    Depositor
    Siegfried Musser
    Article
    Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:
    Insert
    mNeonGreen dimer
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Free cysteine at the C-terminus of the dimer
    Promoter
    T7
    Available since
    July 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    178463-plasmid-map-sequence-id-392808

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  37. pHR-pSFFV-TAF15N-mCherry-sspB

    Plasmid
    #122436
    Purpose
    Expresses fusion of disordered protein TAF15(1-208), fluorescent protein mCherry, and SspB which upon light activation binds to iLID.
    Depositor
    Clifford Brangwynne
    Article
    Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.
    Insert
    TAF15 (TAF15 Human)
    Use
    Lentiviral
    Tags
    mCherry and sspB
    Expression
    Mammalian
    Mutation
    Contains only amino acids 1-208
    Promoter
    SFFV
    Available since
    May 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    122436-plasmid-map-sequence-id-243727

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  38. pcDNA3.1-GGamma2-GFP2

    Plasmid
    #166774
    Purpose
    Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GGamma2-GFP2 (GNG2 Synthetic, Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Contains an N-terminal GFP2 and GSAG linker
    Promoter
    CMV
    Available since
    May 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    166774-plasmid-map-sequence-id-330993

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  39. pcDNA3.1-GGamma12-GFP2

    Plasmid
    #166781
    Purpose
    Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GGamma12-GFP2 (GNG12 Synthetic, Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Contains an N-terminal GFP2 and GSAG linker
    Promoter
    CMV
    Available since
    April 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    166781-plasmid-map-sequence-id-331013

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  40. pcDNA3.1-GGamma5-GFP2

    Plasmid
    #166777
    Purpose
    Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GGamma5-GFP2 (GNG5 Synthetic, Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Contains an N-terminal GFP2 and GSAG linker
    Promoter
    CMV
    Available since
    May 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    166777-plasmid-map-sequence-id-408989

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Showing: 1321 - 1360 of 1450 results