We narrowed to 7,333 results for: ef1a

Showing: 661 - 680 of 7333 results

Results per page:

Sort results by:

qTAG-AAVS1-Ef1a-Puro

Plasmid

#227266

Purpose

Empty AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLv-EF1a-Parkin-A92mKO2

Plasmid

#213559

Purpose

Lentiviral vector for stable expression of Parkin, internally tagged with mKO2

Depositor

Steven Altschuler, Lani Wu

Article

Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

Insert

Parkin (PRKN Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-EF1a-HSF1-GFP

Plasmid

#227820

Purpose

Expresses HSF1 fused to GFP from EF1a promoter

Depositor

Jeffrey Gerst

Article

Pataki et al bioRxiv 2024.10.05.616786

Insert

HSF1 (HSF1 Human)

Use

Lentiviral

Tags

3xHA and GFP

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a-KLF2-T2A-mCHERRY

Plasmid

#224295

Purpose

Expresses mouse KLF2 and mCherry under EF1a promoter

Depositor

Nikhil Joshi

Article

Fagerberg et al Science. 2025 Jan 2;387(6735):eadn2337.

Insert

KLF2 T2A mCherry (Klf2 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

EF1a

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAID-EF1a linearizing in pX330

Plasmid

#140610

Purpose

Crispr/Cas9 plasmid for linearization of pAID-EF1a

Depositor

Tatsuo Fukagawa

Article

Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

Insert

sgRNA for pAID-EF1a plasmid digestion

Use

CRISPR

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-EF1a-pMagHigh-FlpC

Plasmid

#180586

Purpose

PA-Flp

Depositor

Won Do Heo

Article

Jung et al Nat Commun. 2019 Jan 18;10(1):314. doi:

Insert

Split FlpO

Use

AAV

Available Since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEf1a-superDn29-P2A-GFP

Plasmid

#247152

Purpose

Mammalian expression of a human codon optimized engineered superDn29 recombinase (highest efficiency variant)

Depositor

Patrick Hsu

Article

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

superDn29-P2A-GFP

Tags

6xHis

Expression

Mammalian

Mutation

M6I/E70G/A224P/G227V/I233K/K248R/I303K/Q332K/N341…

Promoter

Ef1a

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-EF1a-FLEX-GTB

Plasmid

#26197

Depositor

Edward Callaway

Article

Haubensak et al Nature. 2010 Nov 11. 468(7321):270-6.

Insert

Enhanced green fluorescent protein, TVA, rabies B19 glycoprotein

Use

AAV and Cre/Lox; Adeno-associated virus

Mutation

TVA, rabies glycoprotein are mammalian codon opti…

Available Since

Nov. 11, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-EF1a-4mtGCaMP6f-DIO

Plasmid

#179529

Purpose

Cre-dependent expression of mitochondria-targeted GCaMP6f under control of the EF1a promoter

Depositor

Maarten Kole

Article

Kole et al Nat Commun. 2022 Dec 9;13(1):7598. doi:

Insert

4mtGCaMP6f

Use

AAV

Tags

4x Cox8 targeting sequence

Promoter

Ef1a

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
hAAVS1-gs4_EF1a-Cas9-2A-GFP

Plasmid

#118414

Purpose

CRISPR knockout, expresses Cas9, and sgRNA targeting AAVS1 locus

Depositor

Mark Denham

Article

Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

Insert

gRNA AAVS1 (AAVS1 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-EF1aL-WT_ABCA3-GFP

Plasmid

#188548

Purpose

Lentiviral vector allowing for EF1aL-driven constitutive expression of wildtype human ABCA3:GFP fusion protein

Depositor

Darrell Kotton

Article

Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

Insert

ABCA3:GFP (ABCA3 Human)

Use

Lentiviral

Tags

GFP

Promoter

EF1aL

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-EF1a-fDIO-Cre

Plasmid

#121675

Purpose

Expresses Cre recombinase and HA tag in a Flp dependent fashion (fDIO)

Depositor

Esteban Engel, Alexander Nectow

Article

Schneeberger et al Cell. 2019 Jun 24. pii: S0092-8674(19)30

Has Service

AAV Retrograde, AAV1, AAV5, AAV8, and AAV9

Insert

NLS-CRE-HA

Use

AAV

Tags

3xHA and SV40-NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pT3-Neo-EF1a-GFP

Plasmid

#69134

Purpose

Sleeping Beaquty (SB) vector encoding G418 resistance and GFP from two separate promoters

Depositor

Martin Bonamino

Article

Chicaybam et al Front Bioeng Biotechnol. 2017 Jan 23;4:9

Type

Empty backbone

Use

Sleeping beauty transposon

Expression

Mammalian

Promoter

U3MSCV

Available Since

Jan. 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAF0466 Ef1a-Nm60_attB acceptor

Plasmid

#193476

Purpose

Acceptor plasmid for 3 plasmid recombination assay

Depositor

Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Nm60_attB

Expression

Mammalian

Promoter

Ef1a

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-EF1A-CD19.8H.28TM.28.BB.3z

Plasmid

#200676

Purpose

Modular CD8hinge-CD28transmembrane-CD28-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production

Depositor

Michele Bernasconi

Article

Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

Insert

FMC63-CD8HD-CD28TM-CD28-41BB-CD3z-P2A-EGFP (CD8A, CD28, CD247, TNFRSF9 Human)

Use

Lentiviral

Tags

MycTag

Expression

Mammalian

Promoter

EF1a-short

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
LV_EF1a_CDK4(R24C)-P2A-Hygro_Barcode

Plasmid

#170222

Purpose

Barcoded lentiviral vector to express CDK4 (R24C) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

CDK4 (CDK4 Human)

Use

Lentiviral

Mutation

R24C

Promoter

EF1a

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV x EF1a_EKAREN4-ires-puro

Plasmid

#167828

Purpose

Optimized EKAREV FRET biosensor sensor for ERK

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN4

Use

Lentiviral

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W

Promoter

EF1a

Available Since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMR506-EF1a-H2B-EGFP

Plasmid

#186627

Purpose

Expresses nuclear-localised EGFP under EF1a promoter. For insertion of genetic barcodes into 3'UTR of EGFP.

Depositor

Jonas Frisén

Article

Ratz et al Nat Neurosci. 2022 Mar;25(3):285-294. do

Insert

EF1a (EEF1A1 Synthetic)

Use

Lentiviral

Available Since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEnt L1L3 EF1a-tTA-2

Plasmid

#26803

Depositor

Edward Hsiao

Article

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Insert

Elongation Factor 1 alpha promoter and tTA transgene

Use

Gateway entry vector

Expression

Mammalian

Available Since

Feb. 23, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-EF1alpha-dCas9-p300_Blast

Plasmid

#192653

Purpose

3rd generation lenti vector encoding dCas9-p300 with 2A Blast resistance marker

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

dCas9-p300

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A; H840A in Cas9

Promoter

EF1a

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge