We narrowed to 1,639 results for: plasmid for e coli

1. pZE27GFP2

   Plasmid

   #75455

   Purpose

   Plasmid for constitutive expression of gfp-pdt2

   Depositor

   James Collins

   Article

   Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

   Insert

   gfp-pdt#2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   placIq promoter

   Available since

   Oct. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pZE27GFP4

   Plasmid

   #75457

   Purpose

   Plasmid for constitutive expression of gfp-pdt4

   Depositor

   James Collins

   Article

   Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

   Insert

   gfp-pdt#4
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   placIq promoter

   Available since

   Oct. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pK168.AAV-EF1α-DIO-tTA-P2A-RFP-WPRE (Supernova)

   Plasmid

   #85039

   Purpose

   For AAV-TRE-Cre based Supernova-RFP, pK168 should be used with pK170.

   Depositor

   Takuji Iwasato

   Article

   Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

   Insert

   RFP-P2A-tTA
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pG1AK-sfGFP

   Plasmid

   #71739

   Purpose

   Modular shuttle vector for Geobacillus and E. coli

   Depositor

   Tom Ellis

   Article

   Reeve et al ACS Synth Biol. 2016 Jul 1.

   Insert

   sfGFP
   

   Use

   Synthetic Biology; Shuttle vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pRplsWT

   Available since

   July 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pG1AK-mCherry

   Plasmid

   #71737

   Purpose

   Modular shuttle vector for Geobacillus and E. coli

   Depositor

   Tom Ellis

   Article

   Reeve et al ACS Synth Biol. 2016 Jul 1.

   Insert

   mCherry
   

   Use

   Synthetic Biology; Shuttle vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pRplsWT

   Available since

   July 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. His-DERA

   Plasmid

   #203225

   Purpose

   For protein expression. E. coli 2-deoxy-D-ribose-5-phosphate aldolase. Converts dR5P to G3P and acetaldehyde. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.

   Depositor

   Vern Schramm

   Article

   Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi

   Insert

   deoxyribose aldolase
   

   Use

   Tags

   N-terminal TEV cleavable 6xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pANY2

   Plasmid

   #112196

   Purpose

   This plasmid is used for efficient cloning of DNA and expression of protein in E. coli.

   Depositor

   Yingfeng An

   Article

   Gao et al J Agric Food Chem. 2018 Jun 20;66(24):61

   Type

   Empty backbone

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. amilCP chromoprotein

   Plasmid

   #117847

   Purpose

   BioBrick pSB1C3 plasmid that constitutively overexpresses amilCP chromoprotein in E. coli

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   promoter, RBS, amilCP
   

   Use

   Synthetic Biology; Escherichia coli

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMRBad-C-wtBlc

   Plasmid

   #168257

   Purpose

   C-fragment. This plasmid can be co-transformed with either Plasmid 168259: pET11a-N-wtBlc or Plasmid 168472: pET11a-N-DiB2 to obtain wtBlc‐split or DiB2‐split protein, correspondingly

   Depositor

   Jens Meiler

   Article

   Bozhanova et al FEBS Lett. 2021 Jan;595(2):206-219. doi:

   Insert

   C-fragment of wtBlc
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pOxyR_gfp

    Plasmid

    #194154

    Purpose

    Green Fluorescent Reporter of intracellular hydrogen peroxide

    Depositor

    Sven Panke

    Article

    Kardashliev et al Chem Sci. 2021 Oct 29;12(44):14766-14772

    Insert

    transcriptional regulator, OxyR, cognate promoter PoxyS, fluorescent protein sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PoxyR, PoxyS

    Available since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOxyR_rfp

    Plasmid

    #194155

    Purpose

    Red Fluorescent Reporter of intracellular hydrogen peroxide

    Depositor

    Sven Panke

    Article

    Kardashliev et al Chem Sci. 2021 Oct 29;12(44):14766-14772

    Insert

    transcriptional regulator, OxyR, cognate promoter PoxyS, fluorescent protein mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PoxyR, PoxyS

    Available since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTCF158 Human beta-2 microglobulin

    Plasmid

    #180457

    Purpose

    E. coli expression of class I MHC light chain

    Depositor

    John Altman

    Insert

    Human beta-2 microglobulin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a_PTK2b

    Plasmid

    #224237

    Purpose

    Expression of human PTK2b Kinase in E. coli

    Depositor

    Andrew Mesecar

    Insert

    ptk2b (PTK2B Human)
    

    Use

    Tags

    6xHis and 6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promotor

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET11a_PTK2b_Kinase

    Plasmid

    #224242

    Purpose

    Expression of human PTK2b Kinase domain in E. coli

    Depositor

    Andrew Mesecar

    Insert

    ptk2b_kinase (PTK2B Human)
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promotor

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBS3ndd

    Plasmid

    #97354

    Purpose

    Low background cloning vector for conventional or homologous recombination cloning with sticky blunt or TA compatible ends, kanamycin resistant

    Depositor

    Jamorn Somana

    Insert

    Nucleoid Disruptive Domain
    

    Use

    Cloning

    Tags

    Expression

    Bacterial

    Mutation

    changed methionine 1 to isoleucine

    Promoter

    E. coli lacZ promoter

    Available since

    Aug. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pE-SUMO CRYGB

    Plasmid

    #80751

    Purpose

    Expression of human gamma B-crystallin in E. coli

    Depositor

    Larry David

    Insert

    CRYGB (CRYGB Human)
    

    Use

    Tags

    6xHIS and Smt3 SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pE-SUMO CRYGC

    Plasmid

    #80754

    Purpose

    Expression of human gamma C-crystallin in E. coli

    Depositor

    Larry David

    Insert

    CRYGC (CRYGC Human)
    

    Use

    Tags

    6xHIS and Smt3 SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBS2ndd

    Plasmid

    #97353

    Purpose

    Low background cloning vector for conventional or homologous recombination cloning with sticky blunt or TA compatible ends, ampicillin resistant

    Depositor

    Jamorn Somana

    Insert

    Nucleoid Disruptive Domain
    

    Use

    Cloning

    Tags

    Expression

    Bacterial

    Mutation

    changed methionine 1 to isoleucine

    Promoter

    E. coli lacZ promoter

    Available since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSLC-217

    Plasmid

    #73163

    Purpose

    Template plasmid which encodes a toxin gene (relE) under the control of rhamnose induceable promoter (PrhaB) to be used as a negative selection marker.

    Depositor

    Swaine Chen

    Article

    Khetrapal et al Nucleic Acids Res. 2015 Jul 27;43(13):e8

    Insert

    PrhaB-relE (relE E. coli (bacteria))
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PrhaB

    Available since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRF0G-IodoY

    Plasmid

    #113645

    Purpose

    Plasmid contains the aminoacyl-tRNA synthetase and tRNA genes from methanococcus jannaschii for charging iodotyrosine at amber stop codons.

    Depositor

    Jeffrey Barrick

    Article

    Monk et al ACS Synth Biol. 2016 Sep 20.

    Insert

    IodoYRS and tRNA gene
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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