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pUC57-LbNOX

Plasmid

#75285

Purpose

This plasmid contains human codon optimized sequence of LbNOX which is a water-forming NADH oxidase that can be used to increase NAD+/NADH ratio in mammalian cells.

Depositor

Vamsi Mootha

Publication

Titov et al Science. 2016 Apr 8;352(6282):231-5. doi

Insert

LbNOX

Tags

C-terminal FLAG tag with linker

Mutation

Human codon optimized

Available since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

24 Articles

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tet-pLKO-sgRNA-puro

Plasmid

#104321

Purpose

3rd generation lentiviral plasmid for inducible expression of sgRNA; derived from tet-pLKO-puro; puromycin selection. See manual for detailed protocols.

Depositor

Nathanael Gray

Publication

Huang et al eLife. 2017 Sep 19;6. pii: e26693. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

H1/TO

Available since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pscAAV-GFP

Plasmid

#32396

Depositor

John T Gray

Publication

Gray et al Methods Mol Biol. 2011;807:25-46.

Insert

GFP

Use

AAV; Adeno-associated virus

Expression

Mammalian

Promoter

CMV

Available since

Sept. 28, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

32 Articles

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GFP-ERK1

Plasmid

#14747

Depositor

Rony Seger

Publication

Yung et al J Biol Chem. 2001 Sep 21. 276(38):35280-

Insert

ERK1 (MAPK3 Human)

Tags

EGFP

Expression

Mammalian

Available since

June 12, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pcDNA3 Flag TSC2

Plasmid

#14129

Depositor

Brendan Manning

Publication

Manning et al Mol Cell. 2002 Jul . 10(1):151-62.

Insert

TSC2 (TSC2 Human)

Tags

Flag

Expression

Mammalian

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

21 Articles

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LV-RFP

Plasmid

#26001

Depositor

Elaine Fuchs

Publication

Beronja et al Nat Med. 2010 Jul . 16(7):821-7.

Insert

H2B-RFP (H2BC21 A. victoria, Human)

Use

Lentiviral

Expression

Mammalian

Available since

Dec. 10, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

44 Articles

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pET His6 GST TEV LIC cloning vector (1G)

Plasmid

#29655

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis, GST, and TEV cleavage site

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pComb3XSS

Plasmid

#63890

Purpose

3rd generation plasmid for phage display on modified geneIII, contains stuffer fragment

Depositor

Carlos Barbas

Publication

Andris-Widhopf et al J Immunol Methods. 2000 Aug 28;242(1-2):

Inserts

SS Stuffer
Light Chain Stuffer
Heavy Chain Stuffer

Use

Phage display

Tags

6x His, HA tag, and geneIII

Expression

Bacterial

Promoter

lacZ

Available since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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pEGFP-Q23

Plasmid

#40261

Depositor

David Rubinsztein

Publication

Narain et al J Med Genet. 1999 Oct;36(10):739-46.

Insert

HTT partial exon 1 Q23 (HTT Human)

Tags

EGFP

Expression

Mammalian

Mutation

expanded poly-Q

Promoter

CMV

Available since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

27 Articles

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HDAC7 Flag

Plasmid

#13824

Purpose

Mammalian expression of human histone deacetylase 7 with flag tag

Depositor

Eric Verdin

Publication

Fischle et al J Biol Chem. 1999 Apr 23. 274(17):11713-

Insert

HDAC7 (HDAC7 Human)

Tags

flag

Expression

Mammalian

Available since

Feb. 19, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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ATeam1.03-nD/nA/pcDNA3

Plasmid

#51958

Purpose

mammalian expression of ATeam1.03-nD/nA, a FRET-based ATP indicator

Depositor

Takeharu Nagai

Publication

Kotera et al ACS Chem Biol. 2010 Feb 19;5(2):215-22.

Insert

ATeam1.03-nD/nA

Expression

Mammalian

Available since

March 28, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

24 Articles

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pcDNA Flag Yap1

Plasmid

#18881

Depositor

Yosef Shaul

Publication

Levy et al Mol Cell. 2008 Feb 15. 29(3):350-61.

Insert

Yap1 (YAP1 Human)

Tags

Flag

Expression

Mammalian

Available since

Aug. 14, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

29 Articles

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pcDNA3.1-2xFLAG-SREBP-1c

Plasmid

#26802

Depositor

Timothy Osborne

Publication

Toth et al Mol Cell Biol. 2004 Sep . 24(18):8288-30

Insert

SREBP-1c (SREBF1 Human)

Tags

2XFLAG

Expression

Mammalian

Available since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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pmTurquoise2-ER

Plasmid

#36204

Purpose

In vivo visualization of the endoplasmic reticulum (can be used for colocalization studies)

Depositor

Dorus Gadella

Publication

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

ER-mTurquoise2

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

21 Articles

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YFP-Parkin

Plasmid

#23955

Purpose

Mammalian expression of human Park2 fused to EYFP

Depositor

Richard Youle

Publication

Narendra et al J Cell Biol. 2008 Dec 1. 183(5):795-803.

Insert

Park2 (PRKN Human)

Tags

eYFP

Expression

Mammalian

Available since

April 9, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

51 Articles

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pCMV CEPIA2mt

Plasmid

#58218

Purpose

Green fluorescent indicator for calcium imaging in the mitochondria

Depositor

Masamitsu Iino

Publication

Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

Insert

CEPIA2mt

Tags

myc

Expression

Mammalian

Mutation

GCaMP2 N263Y E282V M339L

Promoter

CMV

Available since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

27 Articles

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EF.STAT3DN.Ubc.GFP

Plasmid

#24984

Purpose

Depositor

Linzhao Cheng

Publication

Hillion et al Cancer Research 68, 10121, December 2008

Insert

STAT3 (STAT3 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Y705F--dominant negative

Available since

June 14, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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pcDNA3.1-mCherry

Plasmid

#128744

Purpose

Expresses mCherry in mammalian cells

Depositor

David Bartel

Publication

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Type

Empty backbone

Expression

Mammalian

Available since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles

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7M8

Plasmid

#64839

Purpose

This plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the 7M8 capsid protein.

Depositor

John Flannery, David Schaffer

Publication

Dalkara et al Sci Transl Med. 2013 Jun 12;5(189):189ra

Insert

7M8 cap

Use

AAV

Mutation

7mer insertion in the AAV2 cap

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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pEGFP-parkin WT

Plasmid

#45875

Depositor

Edward Fon

Publication

Trempe et al Science. 2013 May 9.

Insert

Parkin (PRKN Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

31 Articles

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