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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 6781 - 6800 of 9089 results

  1. pCas3

    Plasmid
    #229538
    Purpose
    pMV_hyg encoding Cas3(wt) and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning.
    Depositor
    Kevin Verstrepen
    Article
    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:
    Insert
    Cas3
    Use
    CRISPR
    Expression
    Bacterial and Yeast
    Mutation
    codon optimized for S.cerevisiae
    Available Since
    Jan. 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    229538-plasmid-map-sequence-id-459007

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  3. RNF168-Halo HRD

    Plasmid
    #207080
    Purpose
    Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous RNF168 locus.
    Depositor
    Jens Schmidt
    Article
    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75
    Insert
    HaloTag followed by a PolyA signal and PuroR cassette flanked by human RNF168 locus sequences
    Expression
    Mammalian
    Promoter
    None
    Available Since
    May 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    207080-plasmid-map-sequence-id-432323

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  4. pAEF5

    Plasmid
    #136305
    Purpose
    Plasmid encoding the Cas9 gene and a gRNA expression cassette allowing to clone a single or multiple gRNAs for DSBs induction in yeast. Constructed by Aubin Fleiss
    Depositor
    Gilles Fischer
    Article
    Agier et al Methods Mol Biol. 2021;2196:181-198. doi
    Type
    Empty backbone
    Use
    CRISPR
    Available Since
    Feb. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    136305-plasmid-map-sequence-id-271550

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  7. pGL4-Gateway-SCP1

    Plasmid
    #71510
    Purpose
    humanSTARR-seq validation vector with Gateway cassette upstream of the firefly luciferase gene and the SCP1 core promoter
    Depositor
    Alexander Stark
    Article
    Arnold et al Science. 2013 Mar 1;339(6123):1074-7. do
    Type
    Empty backbone
    Use
    Humanstarr-seq validation vector
    Tags
    luciferase
    Expression
    Mammalian
    Available Since
    Jan. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 6781 - 6800 of 9089 results