170,231 results

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HIF-TRE-mStrawberry reporter

Plasmid

#158681

Purpose

This plasmid is a HIF1A pathway reporter. It has a 6 x HIF binding element promoter driving the expression of mStrawberry-pGK-BSD

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

mStrawberry

Use

Lentiviral

Expression

Mammalian

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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Flag-Vac 14 Full Length

Plasmid

#47418

Depositor

Peter McPherson

Article

Lemaire et al FEBS Lett. 2006 Dec 22;580(30):6948-54.

Insert

Vac14 (VAC14 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 29, 2014

Availability

Academic Institutions and Nonprofits only
pMpGWB401

Plasmid

#68666

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Expression

Plant

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST Halo-flag-CAD

Plasmid

#188117

Purpose

Expresses N-terminal Halo-tagged/C-terminal flag-tagged human CAD in mammalian cells

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag and Halo tag

Expression

Mammalian

Mutation

TCC -> AGT silent mutations at nt439-441 elimi…

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pKMV-VioC

Plasmid

#65349

Purpose

systhesis of vioC gene in the violacein pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

vioC

Use

Synthetic Biology

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

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TRUPATH Triple Ga12

Plasmid

#196061

Purpose

Encodes a G alpha subunit (GNA12) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

Depositor

Justin English

Article

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Inserts

GAlpha12-RLuc8 (GNA12 Human)
GGamma9-GFP2
GBeta3 (GNB3 Human)

Use

Luciferase

Tags

GFP2 and GSAG linker

Expression

Mammalian

Mutation

RLuc8 and flanking SGGGGS linkers have been inser…

Promoter

CMV

Available Since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-DeAct-SpvB

Plasmid

#89446

Purpose

Expresses EGFP-SpvB (Salmonella SpvB 375-591) in mammalian cells

Depositor

Brad Zuchero

Article

Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

Insert

DeAct-SpvB (spvB Salmonella enterica)

Tags

EGFP

Expression

Mammalian

Mutation

Truncation spanning SpvB amino acids 375-591 (mon…

Promoter

CMV

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a Cdk2ap1CAN

Plasmid

#178032

Purpose

Expression vector for the purpose of protein purification.

Depositor

Lin He

Article

Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

Insert

Cdk2ap1 (Cdk2ap1 Mouse)

Use

Nonviral

Tags

6xHIS - Thrombin - MBP- TEV-TRS

Expression

Bacterial

Promoter

T7LacI

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-Flag-hGSDMB

Plasmid

#218936

Purpose

Expression of N-terminal FLAG tagged human gasdermin B

Depositor

Christopher LaRock

Article

LaRock et al Nature. 2022 May;605(7910):527-531. doi:

Insert

human GSDMB (GSDMB Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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Cenp-B INCENP mCherry

Plasmid

#45235

Purpose

Target INCENP to centromeres via full length CENP-B

Depositor

Michael Lampson

Article

Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.

Insert

Cenp-B, INCENP-Δcen, mCherry

Tags

full length CENP-B and mCherry

Expression

Mammalian

Mutation

CEN box deleted from INCENP (aa1-46)

Available Since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV 3xFLAG-GABARAPL2 WT

Plasmid

#123103

Purpose

Expresses 3xFLAG-GABARAPL2 wild-type in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-PDGFRA-V5/HIS

Plasmid

#201987

Purpose

expression of human PDGFRA receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

PDGFRA receptor tyrosine kinase (PDGFRA Human)

Tags

V5, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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pTET GFP10-FRB/FKBP-GFP11

Plasmid

#182497

Purpose

Tripartite split-GFP. E. coli vector. Inducible coexpression of GFP10-FRB and FKBP-GFP11 coiled coils

Depositor

Stéphanie Cabantous

Article

Pedelacq et al Methods Mol Biol. 2019;2025:423-437. doi

Inserts

FRB
FKBP (Fkbp1a Synthetic, Mouse)

Tags

split GFP10 tripartite and split GFP11 tripartite

Expression

Bacterial

Promoter

TET

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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pJRH-1180 U6-reci Gag-pol v2

Plasmid

#201915

Purpose

Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

Gag-pol

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223-RPS6KC1

Plasmid

#23460

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

RPS6KC1 (RPS6KC1 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pTn7-SCOUT22

Plasmid

#200996

Purpose

pLv2-pUC18R6KT-miniTn7-Km, KmR, ApR

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Type

Empty backbone

Expression

Bacterial

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMEX174-hCALML3

Plasmid

#47600

Depositor

Emanuel Strehler

Article

Qian et al Protein Sci. 1998 Nov;7(11):2421-30.

Insert

CALM3 (CALML3 Human)

Expression

Bacterial

Promoter

T7

Available Since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only
pTRIP TRE BI_GFP11-14-SEPT7_SEPT9_i1-14-GFP10

Plasmid

#180348

Purpose

mammalian co-expression of human SEPT9_i1 fused to GFP10 and of human SEPT7 fused to GFP11

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Inserts

SEPTIN7 (SEPTIN7 Human)
SEPTIN9 (SEPTIN9 Human)
IRES-TagBFP

Use

Lentiviral

Tags

GFP10 and GFP11

Expression

Mammalian

Promoter

bidirectional TRE

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pH3FS-GAL1

Plasmid

#85782

Purpose

N-ICE plasmid pH3FS-GAL1

Depositor

Scott Briggs

Article

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-HphMX-loxP-GAL1p-3FLAG

Use

Cre/Lox

Expression

Yeast

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pCW57.1-DUX4-WT

Plasmid

#99282

Purpose

All-in-one lentivector with tet-inducible DUX4 (wildetype nucleotide sequence)

Depositor

Stephen Tapscott

Article

Jagannathan et al Hum Mol Genet. 2016 Oct 15;25(20):4419-4

Insert

DUX4 (DUX4 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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