59,544 results

1. pML334_RAB7A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227899

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   RAB7A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
   

   Use

   CRISPR

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAC150-FAM134C-GFP

   Plasmid

   #201932

   Purpose

   PiggyBac Vector expressing FAM134C-GFP

   Depositor

   Wade Harper

   Article

   Hoyer et al Nat Cell Biol. 2024 Mar;26(3):378-392. d

   Insert

   FAM134C (RETREG3 Human)
   

   Expression

   Mammalian

   Available Since

   Jan. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBabe_puro_DEST_Flag_PLD1

   Plasmid

   #45268

   Depositor

   William Hahn

   Article

   Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

   Insert

   PLD1 (PLD1 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   May 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. TFORF2944

   Plasmid

   #144420

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   SATB2 (SATB2 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. c-myc promoter (Del 3)

   Plasmid

   #16603

   Depositor

   Bert Vogelstein

   Article

   He et al Science. 1998 Sep 4. 281(5382):1509-12.

   Insert

   c-myc promoter (Del 3) (MYC Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Del-3 (AccI->PvuII). The AccI site is at -741 …

   Available Since

   March 24, 2008

   Availability

   Academic Institutions and Nonprofits only

6. pCMV-STAT6-IRES-Neo

   Plasmid

   #35482

   Depositor

   Axel Nohturfft

   Insert

   STAT6 (STAT6 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. KE-009

   Plasmid

   #133441

   Purpose

   Human elF4H, residue 1-228 (full-length), N-terminal His, GB1, TEV; in pET21 backbone.

   Depositor

   Gerhard Wagner

   Insert

   EIF4H (EIF4H Human)
   

   Expression

   Bacterial

   Available Since

   Jan. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pML531_TOMM20-C-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227890

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   TOMM20 homology arms with mEGFP-3xHA for insertion at C terminus
   

   Use

   CRISPR

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. CDK12 gRNA (BRDN0001147294)

   Plasmid

   #75916

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human CDK12

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   CDK12 (CDK12 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. ZsGreen1-cMYC/pLVX-Puromycin

    Plasmid

    #180278

    Purpose

    Plasmid encoding human c-MYC for reporter assays. Polycistronic puromycin selectable lentiviral vector (unmodified, from TAKARA BIO).

    Depositor

    Andre Catic

    Article

    Moree et al Cancers (Basel). 2022 Feb 4;14(3). pii:

    Insert

    ZsGreen1-cMYC fusion protein MYC (MYC Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pc DNA FRT TO- APP695

    Plasmid

    #114193

    Purpose

    Creation of a stable cell line expressing APP mutation (AD in vitro study)

    Depositor

    Aleksandra Radenovic

    Insert

    Amyloid-Precursor-Protein (APP Human)
    

    Expression

    Mammalian

    Mutation

    wild type splice variant 695: 695AA (2085bp, lack…

    Promoter

    CMV

    Available Since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. mEGFP-N1-LATS2

    Plasmid

    #166474

    Purpose

    Expresses fusion of mEGFP and LATS2

    Depositor

    Shuguo Sun

    Article

    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

    Insert

    mEGFP, LATS2 (LATS2 Human)
    

    Expression

    Mammalian

    Available Since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCI HA NEDD4 DD

    Plasmid

    #26999

    Depositor

    Joan Massague

    Article

    Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

    Insert

    NEDD4 (NEDD4 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    C867A

    Available Since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX-DAXX-His

    Plasmid

    #175782

    Purpose

    DAXX protein for bacteria expression system

    Depositor

    Xiaolu Yang

    Article

    Huang et al Nature. 2021 Aug 18. pii: 10.1038/s41586

    Insert

    death domain associated protein (DAXX Human)
    

    Tags

    GST and TEV

    Expression

    Bacterial

    Available Since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR223_MAPK1_WT

    Plasmid

    #82145

    Purpose

    Gateway Donor vector containing MAPK1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    MAPK1 (MAPK1 Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-GfaABC1D-GRAB_ATP1.0mut

    Plasmid

    #167580

    Purpose

    Express the ATP-insensitive control sensor GRAB_ATP1.0mut in astrocytes

    Depositor

    Yulong Li

    Article

    Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

    Insert

    ATP-insensitive sensor GRAB_ATP1.0mut (control sensor) (P2RY1 Human)
    

    Use

    AAV

    Promoter

    GfaABC1D

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRK5M-TIMP3

    Plasmid

    #31715

    Depositor

    Rik Derynck

    Article

    Liu et al Mol Cell. 2009 Jul 10. 35(1):26-36.

    Insert

    TIMP3 (TIMP3 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-GABRA1-IRES2-EGFP

    Plasmid

    #170820

    Purpose

    human GABAA receptors alpha1 subunit and IRES2-EGFP

    Depositor

    Tingwei Mu

    Article

    Fu et al PLoS One. 2018 Nov 27;13(11):e0207948. d

    Insert

    Human GABAA receptor alpha1 subunit (GABRA1 Human)
    

    Tags

    IRES2-EGFP

    Expression

    Mammalian

    Available Since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR221-SLC7A5_STOP

    Plasmid

    #161363

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC7A5 (SLC7A5 Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 15, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pRST7-ER beta (short)

    Plasmid

    #11357

    Depositor

    Donald McDonnell

    Article

    Hall et al Endocrinology. 1999 Dec . 140(12):5566-7

    Insert

    ER beta short (ESR2 Human)
    

    Expression

    Mammalian

    Available Since

    March 10, 2006

    Availability

    Academic Institutions and Nonprofits only