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pET SUMO-syt7 C2A-2A 2B

Plasmid

#213626

Purpose

Bacterial expression of SUMO-tagged mouse syt7 C2A-2A 2B

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

Insert

syt7 C2A-2A 2B (Syt7 Mouse)

Use

Tags

SUMO

Expression

Bacterial

Mutation

F167A, F229A, R363H

Promoter

Available since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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pET SUMO-FL-syt1 5W

Plasmid

#213629

Purpose

Bacterial expression of SUMO-tagged rat FL-syt1 5W

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

Insert

FL-syt1 5W (Syt1 Rat)

Use

Tags

SUMO

Expression

Bacterial

Mutation

M173W, F231W, F234W, C277A, V304W, Y364W, I367C

Promoter

Available since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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pET SUMO-cPLA2 C2 domain (Y96C)

Plasmid

#213633

Purpose

Bacterial expression of SUMO-tagged human cPLA2 C2 domain (Y96C)

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

Insert

cPLA2 C2 domain (Y96C) (PLA2G4A Human)

Use

Tags

SUMO

Expression

Bacterial

Mutation

Y96C, C139A, C141S

Promoter

Available since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1-Cdc45

Plasmid

#208796

Purpose

Xenopus laevis FLAG5-His10-TEV-Cdc45

Depositor

Aga Gambus

Article

Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

Insert

Cdc45 (cdc45.L Frog)

Use

Tags

FLAG and His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-DONSON-WM

Plasmid

#208798

Purpose

Xenopus laevis His6-Myc-DONSON (W234L, M463T)

Depositor

Aga Gambus

Article

Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

Insert

DONSON (donson.L Frog)

Use

Tags

His and Myc

Expression

Bacterial

Mutation

W234L; M463T

Promoter

T7

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-DONSON-DEWS

Plasmid

#208799

Purpose

Xenopus laevis His6-Myc-DONSON (D374A, E377A, W381A, S437A)

Depositor

Aga Gambus

Article

Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

Insert

DONSON (donson.L Frog)

Use

Tags

His and Myc

Expression

Bacterial

Mutation

D374A; E377A; W381A; S437A

Promoter

T7

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-DONSON-3A

Plasmid

#208800

Purpose

Xenopus laevis His6-Myc-DONSON (V473A, R476A, Y481A)

Depositor

Aga Gambus

Article

Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

Insert

DONSON (donson.L Frog)

Use

Tags

His and Myc

Expression

Bacterial

Mutation

V473A; R476A; Y481A

Promoter

T7

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-DONSON-Δ24

Plasmid

#208801

Purpose

Xenopus laevis His6-Myc-DONSON (1-24aa removed)

Depositor

Aga Gambus

Article

Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

Insert

DONSON (donson.L Frog)

Use

Tags

His and Myc

Expression

Bacterial

Mutation

Δ24, deletion of 1-24aa

Promoter

T7

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-DONSON-DEW

Plasmid

#208802

Purpose

Xenopus laevis His6-Myc-DONSON (D374A, E377A, W381A)

Depositor

Aga Gambus

Article

Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

Insert

DONSON (donson.L Frog)

Use

Tags

His and Myc

Expression

Bacterial

Mutation

D374A; E377A; W381A

Promoter

T7

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-DONSON-ΔD1

Plasmid

#208804

Purpose

Xenopus laevis His6-DONSON (1-155aa removed)

Depositor

Aga Gambus

Article

Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

Insert

DONSON (donson.L Frog)

Use

Tags

His

Expression

Bacterial

Mutation

ΔD1, deletion of 1-155aa

Promoter

T7

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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SpyTag003(M115G)-Talin rod-mCherry

Plasmid

#210026

Purpose

Expression of SpyTag003(M115G)-Talin rod(434-2541)-mCherry in mammalian cells

Depositor

Vesa Hytönen

Article

Rahikainen et al J Am Chem Soc. 2023 Nov 15;145(45):24459

Insert

SpyTag003(M115G)-Talin1 rod domain(434-2541)-mCherry

Use

Tags

Expression

Mammalian

Mutation

SpyTag003 M115G mutation to modify SpyCatcher003 …

Promoter

CMV

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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SpyTag003(M115A)-Talin rod-mCherry

Plasmid

#210025

Purpose

Expression of SpyTag003(M115A)-Talin rod(434-2541)-mCherry in mammalian cells

Depositor

Vesa Hytönen

Article

Rahikainen et al J Am Chem Soc. 2023 Nov 15;145(45):24459

Insert

SpyTag003(M115A)-Talin1 rod domain(434-2541)-mCherry

Use

Tags

Expression

Mammalian

Mutation

SpyTag003 M115A mutation to modify SpyCatcher003 …

Promoter

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-SUMO-B'-l2-B'

Plasmid

#209119

Purpose

Bacterial expression of CC-GEMS ligand SUMO-B'-l2-B'

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

SUMO-B'-l2-B'

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLeo-A-GEMS(JAK-STAT)

Plasmid

#209109

Purpose

Transient mammalian expression of the CC functionalized GEMS receptor A-GEMS (JAK-STAT)

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

A-GEMS(JAK-STAT)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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WT_IFL_A28C_R

Plasmid

#200184

Purpose

WT PA14 I-F Leader and A28C Repeat

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Wildtype I-F Leader and a mutant I-F Locus (A28C)

Use

Tags

Expression

Bacterial

Mutation

A28C in the I-F repeat

Promoter

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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PB-CAG-4300-L1-G1397N

Plasmid

#210396

Purpose

To correct m.A4300G mutation on mtDNA

Depositor

Bin Shen

Article

Chen et al Commun Biol. 2023 Nov 3;6(1):1116. doi:

Insert

4300-L1-G1397N

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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PB-CAG-4300-H2-G1397C

Plasmid

#210395

Purpose

To correct m.A4300G mutation on mtDNA

Depositor

Bin Shen

Article

Chen et al Commun Biol. 2023 Nov 3;6(1):1116. doi:

Insert

4300-H2-G1397C

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a(+)-SUMO-G'-l2-G'

Plasmid

#209120

Purpose

Bacterial expression of CC-GEMS ligand SUMO-G'-l2-G'

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

SUMO-G'-l2-G'

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLeo-A-a3-GEMS(JAK-STAT)

Plasmid

#209111

Purpose

Transient mammalian expression of the CC functionalized GEMS receptor A-a3-GEMS (JAK-STAT)

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

A-a3-GEMS(JAK-STAT)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 IntS12

Plasmid

#210521

Purpose

Expresses GST-tagged Drosophila IntS12

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

IntS12 (IntS12 Fly)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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