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TRE-VEGFA EF1a-rtTA3

Plasmid

#195881

Purpose

Lentiviral construct of doxycycline inducible VEGFA

Depositor

Elaine Fuchs

Article

Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

Insert

VEGFA (Vegfa Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE3GS

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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QQR1 variant

Plasmid

#138564

Purpose

Expresses human codon-optimized SpCas9-QQR1 and blasticidin resistance: EFS promoter-QQR1-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

Insert

QQR1

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

G1218R, N1286Q, I1331F, D1332K, R1333Q, R1335Q, T…

Promoter

EFS

Available Since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-HA_WT POLI

Plasmid

#201440

Purpose

Expresses human DNA polymerase iota with an N-terminal HA tag in mammalian cells

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

DNA polymerase iota (POLI Human)

Tags

HA

Expression

Mammalian

Mutation

Coding sequence has been optimised for expression…

Promoter

CMV

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLN539 (FuGW-G8p-antiPD1(HC)-P2A-antiPD1(LC)-1Pe)

Plasmid

#105200

Purpose

Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of anti-PD1 Ab transcripts containing miR1-Bs(Pe)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

G8p-antiPD1(HC)-P2A-antiPD1(LC)

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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EF1a_FOXA1_P2A_Hygro_Barcode

Plasmid

#120438

Purpose

Barcoded lentiviral vector to express FOXA1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

FOXA1 (FOXA1 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-FLCN

Plasmid

#72291

Purpose

overexpression

Depositor

David Sabatini

Article

Tsun et al Mol Cell. 2013 Nov 21;52(4):495-505. doi

Insert

FLCN (FLCN Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pJH2685

Plasmid

#191372

Purpose

Punc-4-TeTx::mCherry unc-54 3' UTR C.elegans A MNs/others neurou expression of TeTx RFP

Depositor

Mei Zhen

Article

Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

Insert

Tetanus Toxin Light Chain

Tags

wCherry

Expression

Worm

Promoter

Punc-4

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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SpCas9-NG

Plasmid

#138566

Purpose

Expresses human codon-optimized rationally engineered SpCas9 variant and blasticidin resistance: EFS promoter-SpCas9-NG-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

Insert

SpCas9-NG

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

L1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T…

Promoter

EFS

Available Since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_3C-Twin-Strep_IRES-EmGFP

Plasmid

#113884

Purpose

Mammalian (inducible) protein expression, bicistronic expression of cytosolic EmGFP

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Twin-Strep

Promoter

pHR-CMV-TetO2

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-FNIP2

Plasmid

#72294

Purpose

overexpression

Depositor

David Sabatini

Article

Tsun et al Mol Cell. 2013 Nov 21;52(4):495-505. doi

Insert

FNIP2 (FNIP2 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pCMV-ABE6.3

Plasmid

#102916

Purpose

expresses ABE6.3 in mammalian cells

Depositor

David Liu

Article

Gaudelli et al Nature. 2017 Nov 23;551(7681):464-471. d

Insert

ABE6.3

Tags

NLS

Expression

Mammalian

Mutation

see manuscript

Promoter

CMV

Available Since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG/ATP9A-HA

Plasmid

#209213

Purpose

Mammalian expression of ATP9A

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

Insert

ATP9A (ATP9A Human)

Tags

HA

Expression

Mammalian

Available Since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pKS016 - pCAGGS-3XFLAG-mKate2-Pds5a-bpA

Plasmid

#156439

Purpose

For transient expression of mouse PDS5A tagged with mKate2

Depositor

Elphege Nora

Article

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

mouse PDS5A (Pds5a Mouse)

Expression

Mammalian

Mutation

none

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pKS021 - pCAGGS-3XFLAG-mKate2-SA1-bpA

Plasmid

#156440

Purpose

For transient expression of mouse SA1 (= STAG1 or SCC3A) tagged with mKate2

Depositor

Elphege Nora

Article

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

mouse SA1 (Stag1 Mouse)

Expression

Mammalian

Mutation

none

Available Since

Sept. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2-Cx43-HA

Plasmid

#49851

Purpose

Encodes human connexin 43 with a C-terminal HA epitope fusion tag

Depositor

Robin Shaw

Article

Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

Insert

Connexin 43 (GJA1 Human)

Tags

HA

Expression

Mammalian

Mutation

Contains several wobble mutations to confer resis…

Promoter

CMV

Available Since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pLIB-GST-3C-CDK1

Plasmid

#177011

Purpose

Generate baculovirus for insect cell expression of CDK1 with N-terminal GST-3C (PreScission)

Depositor

Andrea Musacchio

Article

Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

Insert

CDK1 (CDK1 Human)

Tags

GST-3C

Expression

Insect

Mutation

codon-optimised for insect cell expression

Available Since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pDRM98 mKate2 Blast

Plasmid

#174720

Purpose

Expresses the NLS-tagged mKate2 gene, P2A and the blasticidin resistance gene.

Depositor

Wayne Tilley

Article

Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413

Inserts

mKate2
BSD

Use

Lentiviral

Tags

P2A linker and SV40 NLS with GGS linker

Expression

Mammalian

Promoter

MND and MND (off mKate-P2A)

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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SpCas9-HF1

Plasmid

#138556

Purpose

Expresses human codon-optimized high-fidelity SpCas9 and blasticidin resistance: EFS promoter-SpCas9-HF1-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

Insert

SpCas9-HF1

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

N497A, R661A, Q695A, Q926A

Promoter

EFS

Available Since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-SFFV-PYL1-sfGFP-Emerin

Plasmid

#121532

Purpose

expression of PYL-SfGFP-Emerin, lentivirus vector

Depositor

Stanley Qi

Article

Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

Insert

Emerin (EMD Human)

Use

Lentiviral

Tags

PYL1-SFGFP

Expression

Mammalian

Promoter

SFFV

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-PS9

Plasmid

#177944

Purpose

Transient mammalian expression of eGFP along with SARS-CoV-2 sequence 20080-21171 (PS9) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

eGFP (ORF1ab )

Expression

Mammalian

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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