We narrowed to 23,275 results for: CRISPR

1. pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESneopA

   Plasmid

   #112921

   Purpose

   Gateway entry vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Neomycin resistant marker

   Depositor

   Kosuke Yusa

   Article

   Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

   Insert

   EF1a promoter, dCas9VP64, MS2p65HSF1, IRESneo
   

   Use

   Gateway entry vector

   Expression

   Mammalian

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYPQ155

   Plasmid

   #69306

   Purpose

   Gateway entry vector with AteCas9D10A

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Insert

   AteCas9D10A (Arabidopsis codon optimized)
   

   Use

   CRISPR

   Mutation

   D10A mutation; NLS fusion

   Available Since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. mCas13 Twin-SUMO

   Plasmid

   #199753

   Purpose

   Expresses codon optimized miniature mCas13 for protein expression in E.coli

   Depositor

   Magdy Mahfouz

   Article

   Mahas et al ACS Synth Biol. 2021 Oct 15;10(10):2541-

   Insert

   mCas13
   

   Tags

   SUMO

   Expression

   Bacterial

   Promoter

   LacI

   Available Since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. qTAG-C-miniTurbo-Zeo

   Plasmid

   #207736

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a miniTurbo-2A-Zeo cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. qTAG-N-Zeo-miniTurbo

   Plasmid

   #207703

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-miniTurbo cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pSB700 lenti gRNA KanR neo

   Plasmid

   #167909

   Purpose

   Lentiviral vector for expressing U6 sgRNA cloning plasmid

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Available Since

   Feb. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti_9xD3B_Cluc_Norm

   Plasmid

   #68414

   Purpose

   CRISPR-Display Cypridina Luciferase-2A-Venus YFP "normalizer," lentiviral format.

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   Cypridina Luciferase-2A-Venus YFP
   

   Use

   Lentiviral and Luciferase

   Mutation

   YFP: M153T,V163A,S175G ("Venus") YFP Va…

   Promoter

   minimalCMV

   Available Since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330A_D10A-1x4

   Plasmid

   #58774

   Purpose

   Expresses Cas9 nickase and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 (D10A) nickase
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   D10A nickase-converting mutation

   Promoter

   CBh

   Available Since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCBC-DT1T2.2

   Plasmid

   #134752

   Purpose

   PCR template for sgRNA cloning

   Depositor

   Qi-Jun Chen

   Article

   Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

   Insert

   sgRNA-AtU6_29p
   

   Use

   CRISPR

   Available Since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCBC-MT1T2.2

    Plasmid

    #134751

    Purpose

    PCR template for sgRNA cloning

    Depositor

    Qi-Jun Chen

    Article

    Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

    Insert

    sgRNA-TaU3p
    

    Use

    CRISPR

    Available Since

    Dec. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. p2CT-His-MBP-Ppr_Cas13a_WT

    Plasmid

    #91872

    Purpose

    Bacterial expression for Cas13a

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

    Insert

    PprCas13a (PprC2c2) WT
    

    Tags

    His6-MBP-TEV site

    Expression

    Bacterial

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMOD6-pGRR-r11-r10-yeGFP

    Plasmid

    #74368

    Purpose

    pGRR NOR gate promoter responsive to gRNA-r11 and gRNA-r10. Integrates in the URA locus of W303 and recovers URA function.

    Depositor

    Eric Klavins

    Article

    Gander et al bioRxiv 041871

    Insert

    yeGFP
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Promoter

    pGRR-r11-r10

    Available Since

    April 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pM115: CasRx control presgRNA

    Plasmid

    #166867

    Purpose

    U6-driven expression of control (non-targeting) presgRNA compatible with CasRx.

    Depositor

    Guei-Sheung Liu

    Article

    Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

    Insert

    non-targeting presgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOC1 Gria1-GFP-FRB KI

    Plasmid

    #183428

    Purpose

    CreOFF knock-in for GluA1-GFP-FRB (amino acid position: stop codon)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and GFP-FRB donor
    

    Use

    CRISPR

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOC2 Dlg4-Halo-FKBP KI

    Plasmid

    #183435

    Purpose

    CreOFF knock-in for PSD95-Halo-FKBP (amino acid position: R721)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and Halo-FKBP donor
    

    Use

    CRISPR

    Available Since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. p2CT-His-MBP-Lne_Cas13a_WT

    Plasmid

    #91866

    Purpose

    Bacterial expression for Cas13a

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

    Insert

    LneCas13a (LneC2c2) WT
    

    Tags

    His6-MBP-TEV site

    Expression

    Bacterial

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX330A_D10A-1x6

    Plasmid

    #58776

    Purpose

    Expresses Cas9 nickase and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 (D10A) nickase
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D10A nickase-converting mutation

    Promoter

    CBh

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. p2CT-His-MBP-Lbu_C2c2_R1079A_K1080A

    Plasmid

    #91861

    Purpose

    Bacterial expression for Cas13a

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

    Insert

    LbuCas13a (LbuC2c2) R0179A/K1080A
    

    Tags

    His6-MBP-TEV site

    Expression

    Bacterial

    Mutation

    K1080A

    Available Since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCas5

    Plasmid

    #82378

    Purpose

    sgRNA targeting YFP expressed from pJ23119 in the NS1 locus with kanamycin resistance

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    gRNA
    

    Promoter

    PJ23119

    Available Since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. p2CT-His-MBP-Lbu_C2c2_E299A

    Plasmid

    #91855

    Purpose

    Bacterial expression for Cas13a

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

    Insert

    LbuCas13a (LbuC2c2) E299A
    

    Tags

    His6-MBP-TEV site

    Expression

    Bacterial

    Mutation

    E299A

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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