We narrowed to 7,168 results for: GFP expression plasmids

1. shNg Lenti FHRC3J1UGW

   Plasmid

   #92233

   Purpose

   lentiviral expression of EGFP and Ng shRNA

   Depositor

   Weifeng Xu

   Article

   Hwang et al Biol Psychiatry. 2021 Feb 1;89(3):256-26

   Insert

   shRNA targeting Ng
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   H1

   Available Since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAVS1-Puro-CAG-GCaMP6s

   Plasmid

   #120896

   Purpose

   Donor plasmid targeting human AAVS1 safe harbor locus to generate GCaMP6s gene knockin. Contains two homologous arms flanking T2A-Puro resistance gene and CAG-driven GCaMP6s gene.

   Depositor

   Xiaojun Lian

   Article

   Jiang et al iScience. 2018 Oct 12;9:27-35. doi: 10.1

   Insert

   GCaMP6s
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOPS0381

   Plasmid

   #133231

   Purpose

   Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.

   Depositor

   Philip Poole

   Article

   Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

   Insert

   constructed with constitutive promoter PsNifH (pOGG043), sfGFP (pOGG037) and T-pharma (pOGG003)
   

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Available Since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. mMaple-OMP25

   Plasmid

   #141151

   Purpose

   Photoconvertible fluorescent protein targeted to the outer mitochondrial membrane (OMM) in mammalian cells.

   Depositor

   Gia Voeltz

   Article

   Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Insert

   OMP25 (Synj2bp Rat)
   

   Tags

   mMaple

   Expression

   Mammalian

   Mutation

   OMP25 c terminus from addgene plasmid #69598

   Promoter

   CMV

   Available Since

   Aug. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pASPIre3

   Plasmid

   #154844

   Purpose

   Derivative of pASPIre2 lacking mCherry but containing a silent 150 bp DNA spacer flanked by attB/P sites; main uASPIre plasmid used in this study

   Depositor

   Markus Jeschek

   Article

   Hollerer et al Nat Commun. 2020 Jul 15;11(1):3551. doi:

   Insert

   Bxb1-sfGFP fusion controlled by rhamnose-inducible promoter; attB/attP-flanked silent discriminator
   

   Expression

   Bacterial

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. Lck-mCherry-RGS

   Plasmid

   #112935

   Purpose

   Plasmid encodes a membrane located inhibitor of Ga12/13 signaling (RGS domain from p115)

   Depositor

   Dorus Gadella

   Article

   Reinhard et al Mol Biol Cell. 2017 Nov 7;28(23):3371-33

   Insert

   p115RhoGEF-RGS domain
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAT9750-BEAR-mCherry

   Plasmid

   #162990

   Purpose

   BEAR target plasmid with split mCherry and disrupted 5' splice site

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   mCherry split with an intron between amino acids 119-120
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAT-9222

   Plasmid

   #124222

   Purpose

   Cloning plasmid for EGxFP assay with self-targeting gRNA-s

   Depositor

   Ervin Welker

   Article

   Talas et al Nucleic Acids Res. 2021 Jan 15. pii: 610

   Insert

   self-targeting gRNA between EGFP halves
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Nov. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. FgH1tUTG-sgRNA.FOXA-ZEB2_CRISPRd

   Plasmid

   #216170

   Purpose

   Express the gRNA targeting the FOXA-binding site in the human ZEB2 locus for the CRISPRd experiment

   Depositor

   Makiko Iwafuchi

   Article

   Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

   Insert

   sgRNA- FOXA.bs- ZEB2.locus
   

   Use

   Lentiviral

   Tags

   EGFP

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. FgH1tUTG-sgRNA.Control-ZEB2_CRISPRd

    Plasmid

    #216172

    Purpose

    Express the gRNA targeting the control site in the human ZEB2 locus for the CRISPRd experiment

    Depositor

    Makiko Iwafuchi

    Article

    Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

    Insert

    sgRNA- Control- ZEB2.locus
    

    Use

    Lentiviral

    Tags

    EGFP

    Available Since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pU6-sgRNA EF1Alpha-puro-T2A-BFP

    Plasmid

    #60955

    Purpose

    Parental vector for the CRISPRi/a libraries. Expresses an sgRNA from the U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter

    Depositor

    Jonathan Weissman

    Article

    Gilbert et al Cell. 2014 Oct 23;159(3):647-61. doi: 10

    Inserts

    sgGFP-NT2
    puro-T2A-BFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Jan. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-EF1a-hMLH1dn-Hygro

    Plasmid

    #195512

    Purpose

    Lentiviral expression plasmid of hMLH1dn with P2A-HygroR marker

    Depositor

    Hyongbum Kim

    Article

    Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

    Insert

    hMLH1dn-P2A-HygroR (MLH1 Human)
    

    Use

    Lentiviral

    Tags

    P2A

    Expression

    Mammalian

    Mutation

    Human MLH1 del754-756

    Promoter

    EF-1a

    Available Since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEG302_22aa_SunTag_MQ1(Q147L)_g4+g10+g18

    Plasmid

    #172318

    Purpose

    CRISPR dCas9 SunTag system to target a variant of bacterial DNA methyltransferase MQ1 to install CG specific methylation to the FWA locus with three guide RNAs

    Depositor

    Steven Jacobsen

    Article

    Ghoshal et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

    Insert

    g18_U6_g10_U6_g4_U6_NOS_MQ1(Q147L)_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-CaMKII-GAL4-iLight-VP16

    Plasmid

    #170274

    Purpose

    NLS-Gal4(1-65)-iLight(human codon optimized)-VP16 construct expression in mammalian cells. pAAV vector.

    Depositor

    Vladislav Verkhusha

    Article

    Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

    Insert

    NLS-Gal4(1-65)-iLight(human codon optimized)-VP16
    

    Use

    AAV

    Mutation

    See Comments

    Promoter

    CaMKII

    Available Since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSLED.NPL

    Plasmid

    #193014

    Purpose

    Neuron-specific reporter (CBh promoter, Pls3 exon, bichromatic reporter)

    Depositor

    Jonathan Ling

    Article

    Ling et al Nat Commun. 2022 Oct 1;13(1):5773. doi:

    Insert

    Bichromatic reporter (EGFP and dsRed-Express2)
    

    Use

    AAV

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSLED.GluA2

    Plasmid

    #193017

    Purpose

    GluA2 (Gria2) flip/flop reporter (hSyn promoter, Gria2 flip/flop exons, bichromatic reporter)

    Depositor

    Jonathan Ling

    Article

    Ling et al Nat Commun. 2022 Oct 1;13(1):5773. doi:

    Insert

    Bichromatic reporter (EGFP and dsRed-Express2)
    

    Use

    AAV

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSLED.ENS

    Plasmid

    #193016

    Purpose

    Excitatory neuron-specific reporter (hSyn promoter, Synrg exon, bichromatic reporter)

    Depositor

    Jonathan Ling

    Article

    Ling et al Nat Commun. 2022 Oct 1;13(1):5773. doi:

    Insert

    Bichromatic reporter (EGFP and dsRed-Express2)
    

    Use

    AAV

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSLED.RAB

    Plasmid

    #193015

    Purpose

    Photoreceptor-specific reporter (CBh promoter, Atp1b2 exon, bichromatic reporter)

    Depositor

    Jonathan Ling

    Article

    Ling et al Nat Commun. 2022 Oct 1;13(1):5773. doi:

    Insert

    Bichromatic reporter (EGFP and dsRed-Express2)
    

    Use

    AAV

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLJM1-FIRE-pHLy

    Plasmid

    #170775

    Purpose

    Ratiometric biosensor expressed with CMV promoter for probing lysosomal pH in mammalian cells

    Depositor

    Aimee Kao

    Article

    Chin et al ACS Sens. 2021 Jun 8. doi: 10.1021/acsse

    Insert

    LAMP1 (LAMP1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry and mTFP1

    Expression

    Mammalian

    Mutation

    84 nucleotide Signal Sequence switched to the beg…

    Promoter

    CMV

    Available Since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pmCherry C1 Omp25

    Plasmid

    #157758

    Purpose

    Expression of mcherry-labelled mitochondrial outer membrane protein 25 in cells

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    SYNJ2bp (SYNJ2BP Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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