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CMV:eGFP-p2A-HA-GPR151(p.Arg95Ter)

Plasmid

#190134

Purpose

expresses eGFP-p2A-HA-GPR151 (pArg95Ter variant) in mammalian cells

Depositor

Joshua Knowles

Article

Bielczyk-Maczynska et al Nat Commun. 2022 Dec 1;13(1):7408. doi:

Insert

eGFP-p2A-HA-GPR151(p.Arg95Ter)

Use

Lentiviral

Tags

HA, eGFP, and p2A

Expression

Mammalian

Mutation

p.Arg95Ter mutation

Promoter

CMV

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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Mito-mEosEM-E

Plasmid

#226335

Purpose

Photoswitchable fluorescent protein for correlative light-electron microscopy; mitochondria-targeted mEosEM-E for mammalian expression

Depositor

Pingyong Xu

Article

Peng et al Cells. 2022 Mar 23;11(7):1077. doi: 10.3

Insert

COX8A (COX8A Human)

Tags

mEosEM-E

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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MiniCoopR 2xU6:gRNA pten, mitfa:Cas9

Plasmid

#118845

Purpose

Targets ptena and ptenb and expresses zebrafish mitfa specifically in zebrafish melanocytes

Depositor

Leonard Zon

Article

Ablain et al Science. 2018 Nov 1. pii: science.aau650

Insert

ptena and ptenb (ptena, ptenb Zebrafish)

Use

CRISPR; Tol2

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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MadMyc-HA

Plasmid

#16557

Depositor

Bert Vogelstein

Article

Hermeking et al Proc Natl Acad Sci U S A. 2000 Feb 29. 9

Insert

MADMYC (MYC Human)

Tags

HA

Expression

Mammalian

Mutation

Dominant negative mutant

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3.1+_V5-ANKRD52

Plasmid

#92012

Purpose

Expresses V5-ANKRD52

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

ANKRD52 (ANKRD52 Human)

Tags

V5

Expression

Mammalian

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pSECC-sgTom

Plasmid

#138661

Purpose

Expresses a Tomato-targeting sgRNA, Cas9, and Cre-recombinase

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgTomato

Use

Lentiviral

Promoter

hU6

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pMH-SFB-DYRK1B

Plasmid

#101771

Purpose

Mammalian expression of DYRK1B fusion protein

Depositor

Michael Huen

Article

Dong et al Proc Natl Acad Sci U S A. 2020 Jul 21;11

Insert

DYRK1B / MIRK (DYRK1B Human)

Tags

S peptide-flag-streptavidin binding peptide (SFB)

Expression

Mammalian

Mutation

siRNA-resistant allele

Available Since

Oct. 16, 2017

Availability

Academic Institutions and Nonprofits only
pCDNA5 TEX264wt-cSSH

Plasmid

#220209

Purpose

Mammalian expression of wild type TEX264, C-terminal twin-Strep_HA tag

Depositor

Kristijan Ramadan

Article

Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

Insert

TEX264 (TEX264 Human)

Tags

Strep-strep-HA

Expression

Mammalian

Promoter

CMV/TetO

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-cTNT-Cre-miR122 TS

Plasmid

#209784

Purpose

Expresses Cre by the specific cTNT promoter and incorporation of the miR122 target sequences (miR122TS) into the 3’ untranslated region (3’ UTR)

Depositor

Yuxuan Guo

Article

Yang et al Circulation. 2024 May 28;149(22):1778-17

Insert

miR122 ts

Use

AAV

Promoter

cTNT

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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AID-NG-CBE

Plasmid

#227416

Purpose

C-to-T base editing in mammalian cells

Depositor

Yang Sun

Article

Liu et al Adv Sci (Weinh). 2024 Sep 19:e2405628. d

Insert

AID-nSpCas9-NG-2xUGI (AICDA Human)

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 25, 2024

Availability

Academic Institutions and Nonprofits only

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GA-DEVD-GB-NES

Plasmid

#61020

Purpose

green ddFP based caspase-3 biosensor

Depositor

Robert Campbell

Article

Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

Insert

ddGFP A and ddGFP B

Tags

HindIII and NES sequence LALKLAGLDIGS placed afte…

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pScarlessHD-N-3x127D01-DsRed

Plasmid

#171579

Purpose

For scarless insertion of 3x127D01 NanoTag into N-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

3x127D01 (CXCR2 Human)

Expression

Insect

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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mCardinal-Paxillin-22

Plasmid

#56171

Purpose

Localization: Focal Adhesions, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

Paxillin

Tags

mCardinal

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pAW-CD8-NbVHH05-GFP

Plasmid

#171576

Purpose

Expresses GFP-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-GFP) in fly cell cytomembrane

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

CD8-NbVHH05-GFP (UBE2J1 Human)

Tags

CD8 and GFP

Expression

Insect

Promoter

fly actin5C promoter

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pAG657 H2B-pFAST

Plasmid

#172862

Purpose

Expresses pFAST fused to zebrafish histone H2B in mammalian cells

Depositor

Arnaud Gautier

Article

Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

Insert

H2B-pFAST

Tags

Myc tag (C terminal on insert)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pAG671 mito-pFAST

Plasmid

#172867

Purpose

Expresses mitochondrial targeting domain-pFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

Insert

mito-pFAST

Tags

Myc tag (C terminal on insert)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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Pblu-AAVS1-Cas9-p300-M2rtTA-AAVS1

Plasmid

#112261

Purpose

Donor plasmid with a Cas9-p300 expression cassette, a M2rtTA expression cassette, a T2A-puro selection cassette, and two gRNA recognition sequences at both ends of the whole insertion DNA fragment.

Depositor

Zhili Rong

Article

Ma et al FEBS Lett. 2018 Jun 5. doi: 10.1002/1873

Insert

spCas9-p300 (EP300 Human, Synthetic)

Expression

Mammalian

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro CAG IL13-CAR

Plasmid

#157742

Purpose

knock IL13-CAR into AAVS1 safe harbor locus for constitutive expression

Depositor

Xiaoping Bao

Article

Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

Insert

Chimeric antigen receptor targeting IL13

Use

CRISPR, Synthetic Biology, and TALEN

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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Direct-seq_lentiGuide-Puro-tRNA-Loop2-8A8G

Plasmid

#157986

Purpose

Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

Depositor

Lijia Ma

Article

Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

Insert

S. pyogenes sgRNA cassette (tRNA-Loop2-8A8G)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCT-CMV-UBE3B-copGFP-Puro

Plasmid

#107342

Purpose

Expresses UBE3B fused to copGFP in mammalian cells.

Depositor

Robert Sobol

Article

Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

Insert

Ubiquitin-protein ligase E3B (UBE3B Human)

Use

Lentiviral

Tags

copGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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