We narrowed to 15,731 results for: LEA

1. pCAGGS-SMS2-TEV-Twin-Strep

   Plasmid

   #202529

   Purpose

   Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human Sphingomyelin synthase 2 (SMS2) in mammalian cell

   Depositor

   Chiaki Murakami

   Article

   Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

   Insert

   SGMS2 (SGMS2 Human)
   

   Tags

   TEV cleavable site and Twin-Strep-tag

   Expression

   Mammalian

   Mutation

   deleted stop codon (TGA)

   Promoter

   CAG and chicken β-actin promoter

   Available Since

   July 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pExodus CMV.Artemis

   Plasmid

   #40211

   Purpose

   Mammalian expression of codon-optimized Artemis.

   Depositor

   Andrew Scharenberg

   Article

   Certo et al Nat Methods. 2012 Oct;9(10):973-5. doi:

   Insert

   Artemis (DCLRE1C Human)
   

   Expression

   Bacterial and Mammalian

   Promoter

   CMV and T7

   Available Since

   Sept. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5-FRT/TO-3*Flag-APEX2 C-term SMN2

   Plasmid

   #187587

   Purpose

   Express FLAG epitope and APEX2-tagged SMN2 fusion protein in mammalian cells

   Depositor

   Benjamin Blencowe

   Article

   Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

   Insert

   SMN2 (SMN2 Human)
   

   Tags

   APEX2-FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENTR-actin-V159N

   Plasmid

   #118383

   Purpose

   Gateway compatible donor vector with actin-V159N.

   Depositor

   Maria Vartiainen

   Article

   Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

   Insert

   actin beta (ACTB Human)
   

   Use

   Donor-plasmid

   Mutation

   V159N

   Available Since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGP-CMV-GCaMP6-150

   Plasmid

   #91777

   Purpose

   Mammalian expression of low affinity calcium-sensing fluorescent protein

   Depositor

   GENIE Project, Douglas Kim

   Article

   de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

   Insert

   GCaMP6-150
   

   Tags

   6xHis, T7 epitope, and Xpress tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVH-EF1a-GFP-P2A-OCT4

   Plasmid

   #130692

   Purpose

   Lentiviral plasmids encoding human OCT4 (POU5f1) along with GFP linked via P2A.

   Depositor

   Kejin Hu

   Article

   Shao et al Nat Commun. 2016 Mar 7;7:10869. doi: 10.

   Insert

   human OCT4 (POU5F1 Human)
   

   Use

   Lentiviral

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMT-sox10-cytoBirA-2A-mCherry_Ras

   Plasmid

   #80062

   Purpose

   Sox10 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
   

   Use

   Zebrafish biotagging

   Tags

   3x HA

   Expression

   Bacterial

   Promoter

   Sox10 promoter

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. OCT4-eGFP-2A-Puro

   Plasmid

   #31939

   Depositor

   Rudolf Jaenisch

   Article

   Hockemeyer et al Nat Biotechnol. 2011 Jul 7. doi: 10.1038

   Inserts

   OCT4 (POU5F1 Human)
   eGFP (eGFP )
   

   Use

   Targeting donor

   Tags

   eGFP

   Available Since

   Sept. 13, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. KAT14-pDEST10

   Plasmid

   #118382

   Purpose

   HIS-tagged insect cell expression vector with KAT14.

   Depositor

   Maria Vartiainen

   Article

   Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

   Insert

   KAT14 (KAT14 Human)
   

   Tags

   HIS

   Expression

   Insect

   Promoter

   Polyhedrin

   Available Since

   May 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pWAP-HGF

    Plasmid

    #83503

    Purpose

    Generation of transgenic mice overexpressing the HGF under the control of the WAP gene promoter

    Depositor

    Lothar Hennighausen, Marta Gallego

    Article

    Gallego et al Oncogene. 2003 Nov 20;22(52):8498-508.

    Insert

    HGF (Hgf Mouse)
    

    Use

    Mouse Targeting

    Mutation

    also contains beta-globin sequences from pUC198

    Promoter

    mouse WAP (whey acidic protein)

    Available Since

    Feb. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pbZIP9:GFP-GUS

    Plasmid

    #172483

    Purpose

    GFP-GUS driven by the bZIP9 promoter

    Depositor

    Wolf Frommer

    Article

    Kim et al Plant Cell. 2021 May 5;33(3):511-530. do

    Insert

    bZIP9 promoter (BZIP9 Mustard Weed)
    

    Tags

    GFP and GUS

    Expression

    Plant

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBJG1 T7-8xHis-OGT K842M

    Plasmid

    #154272

    Purpose

    Bacterial expression of full length human OGT with K842M mutation (catalytically inactive). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.

    Depositor

    Suzanne Walker

    Article

    Lazarus et al Nature. 2011 Jan 27;469(7331):564-7. doi

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Tags

    8x His, HRV3C protease site, and T7 leader

    Expression

    Bacterial

    Mutation

    Lysine 842 changed to Methionine- bacterial codon…

    Available Since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. OCT4-eGFP-PGK-Puro

    Plasmid

    #31937

    Purpose

    Oct4 targeting donor vector

    Depositor

    Rudolf Jaenisch

    Article

    Hockemeyer et al Nat Biotechnol. 2011 Jul 7. doi: 10.1038

    Insert

    OCT4 (POU5F1 Human)
    

    Use

    Targeting donor

    Tags

    eGFP

    Available Since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5-FRT/TO-3*Flag-APEX2 N-term WRAP53

    Plasmid

    #187578

    Purpose

    Express FLAG epitope and APEX2-tagged WRAP53 fusion protein in mammalian cells

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Insert

    WRAP53 (WRAP53 Human)
    

    Tags

    APEX2-FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-MCP-SRSF3-V5-mCherry

    Plasmid

    #235090

    Purpose

    SRSF3 mCherry fusion protein with MCP domain

    Depositor

    Mitchell Guttman

    Article

    Bhat et al Nature. 2024 May;629(8014):1165-1173. do

    Insert

    pCAG-MCP-SRSF3-V5-mCherry (SRSF3 Human)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV_eA3A T31A-NG-nCas9

    Plasmid

    #163539

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    eA3A T31A-NG-nCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)eA3A T31ANG (L111R, D1135V, G1218R, E…

    Available Since

    Dec. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. VP16-ER beta (long)

    Plasmid

    #11352

    Depositor

    Donald McDonnell

    Article

    Chang et al Mol Cell Biol. 1999 Dec . 19(12):8226-39

    Insert

    ER beta long (ESR2 Human)
    

    Expression

    Mammalian

    Available Since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. pclbw-opa1(isoform 1)-myc

    Plasmid

    #62845

    Purpose

    Mammalian Expression of myc-tagged Opa1 isoform1

    Depositor

    David Chan

    Article

    Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi

    Insert

    mmOPA1isoform1-myc (Opa1 Mouse)
    

    Use

    Retroviral

    Tags

    9xMyc

    Expression

    Mammalian

    Promoter

    cmv-bactin

    Available Since

    Jan. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pAAV2-CAG-mGreenLantern-KASH

    Plasmid

    #191094

    Purpose

    To express a bright monomeric green FP to label the eucaryotic nuclear envelope. To be used in tissue clearing methods and other fluorescent microscopy methods

    Depositor

    Murray Blackmore, Pantelis Tsoulfas

    Article

    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

    Insert

    mGreenLantern-KASH
    

    Use

    AAV and Synthetic Biology

    Tags

    mGreenLantern fused to the KASH domain of Nesprin…

    Expression

    Mammalian

    Mutation

    Optimizzation was done using the Human codon usage

    Promoter

    CMV enhancer and CAG

    Available Since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSynapsin_NLS_GFP_P2A_His6_GluA2

    Plasmid

    #170453

    Purpose

    Expresses histidine tagged GluA2 in neuronal cells

    Depositor

    Shigeki Watanabe

    Article

    Li et al Nat Commun. 2021 Jan 29;12(1):677. doi:

    Insert

    GluA2 (Gria2 Mouse)
    

    Use

    Lentiviral

    Tags

    Hexa-histidine

    Expression

    Mammalian

    Promoter

    Synapsin

    Available Since

    July 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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