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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11,809 results for: emb

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Showing: 6881 - 6900 of 11809 results

  2. pcry:GFP,-503unc:hACTA1GFP

    Plasmid
    #172529
    Purpose
    alphaA-crystallin promoter (cry) drives GFP expression in the lens. The 503bp unc-45b muscle-specific promoter drives expression of GFP-tagged human ACTA1.
    Depositor
    Joachim Berger
    Publication
    Berger et al Cell Rep. 2018 Jan 9;22(2):313-322. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ATCA1 (ACTA1 Human)
    Expression
    Mammalian
    Available since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172529-plasmid-map-sequence-id-341033

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  3. pET3d-SF-DCTN1-TS

    Plasmid
    #169375
    Purpose
    E. coli expression plasmid for human DCTN1 (longest transcript variant p150glued/isoform 1), with an N-terminal splitFlAsH tag and a C-terminal TwinStrep tag.
    Depositor
    Bernd Bukau
    Publication
    Bertolini et al Science. 2021 Jan 1;371(6524):57-64. doi
    Insert
    dynactin subunit 1 isoform 1 (DCTN1 Human)
    Tags
    6xHis Tag, SplitFlAsH Tag, and TwinStrep Tag
    Expression
    Bacterial
    Mutation
    Point mutations: A139T, I304T, Q322R, T516A, E577…
    Available since
    May 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    169375-plasmid-map-sequence-id-333771

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  4. pBB0323-TEV-His12

    Plasmid
    #137039
    Purpose
    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BB0323 with C-terminal TEV-protease-cleavable His12
    Depositor
    Debra Hansen
    Publication
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66700.1
    Tags
    His12 (TEV protease cleavable)
    Expression
    Bacterial
    Mutation
    wild type, full-length protein, contains signal p…
    Promoter
    T7
    Available since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    137039-plasmid-map-sequence-id-291948

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  5. prNFL-P8R-EGFP

    Plasmid
    #132598
    Purpose
    Expresses EGFP-tagged rat NFL containing the Charcot-Marie-Tooth disease type 2E P8R mutation
    Depositor
    Anthony Brown
    Publication
    Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Nefl (Nefl Rat)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    23C>G; Pro8Arg
    Promoter
    CMV
    Available since
    Nov. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    132598-plasmid-map-sequence-id-260971

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  6. prNFL-E397K-EGFP

    Plasmid
    #132603
    Purpose
    Expresses EGFP-tagged rat NFL containing the Charcot-Marie-Tooth disease type 2E E397K mutation
    Depositor
    Anthony Brown
    Publication
    Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Nefl (Nefl Rat)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    1189G>A; Glu397Lys
    Promoter
    CMV
    Available since
    Nov. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    132603-plasmid-map-sequence-id-260029

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  7. pTIGER_tdTomato::3xFLAG::dCrk

    Plasmid
    #131138
    Purpose
    UASp construct for over-expression of Drosophila Crk with N-terminal tandem Tomato and 3X FLAG tag. Compatible with PhiC31-mediated site-specific integration or classical P-element insertion.
    Depositor
    Mark Peifer
    Publication
    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.
    Insert
    Crk (Crk Fly)
    Tags
    3XFLAG and tdTomato
    Expression
    Insect
    Available since
    Oct. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    131138-plasmid-map-sequence-id-258855

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  10. pUC19-K1LM04

    Plasmid
    #103119
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Publication
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    K1LM04(Cas9 coding gene from Bergeyella zoohelcum ATCC 43767)
    Use
    CRISPR; Cloning vector
    Mutation
    human codon-optimized
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103119-plasmid-map-sequence-id-201919

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  11. pUC19-E3CY56

    Plasmid
    #103075
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Publication
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    E3CY56(Cas9 coding gene from Aminomonas paucivorans DSM 12260)
    Use
    CRISPR; Cloning vector
    Mutation
    human codon-optimized
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103075-plasmid-map-sequence-id-201714

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  18. PR594-His-bio

    Plasmid
    #52332
    Purpose
    Expresses full-length Neuron-specific protein family member 1 ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.
    Depositor
    Gavin Wright
    Publication
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    PR594 (NSG1 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    52332-plasmid-map-sequence-id-81052

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  19. pCMV-HA-mIFITM3-K83,88,104A

    Plasmid
    #58405
    Purpose
    Expresses murine IFITM3-K83,88,104A with an N-terminal HA tag in mammalian cells
    Depositor
    Howard Hang, Jacob Yount
    Publication
    Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    IFITM3 (Ifitm3 Mouse)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Lysines 83, 88, and 104 to Alanine
    Promoter
    CMV IE
    Available since
    Sept. 8, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 6881 - 6900 of 11809 results