We narrowed to 11,014 results for: phen
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Plasmid#203517PurposeGateway-compatible destination vector for yeast expression with GAL1 promoter (2x GAL4 binding sites)/upstream ORF/6stop mutation/URA3 markerDepositorTypeEmpty backboneExpressionYeastAvailable SinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only
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pRM27
Plasmid#163695Purposeubb promoter driven expression of lck:mNeonGreenDepositorAvailable SinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCYC1pr-GNSI (LEU)
Plasmid#111450PurposeGNSI is a synthetic, genetically-encoded reporter that allows rapid plate-based assessment of AP-3 functional deficiency, using either colorimetric or growth phenotype readouts.DepositorInsertsCYC1 Promoter (CYC1 Budding Yeast)
Nyv1 Cytosolic Domain (aa 6-230)
Snc1 Transmembrane Domain (TMD)
SUC2 CDS
SUC2 terminator
TagsmGFP5ExpressionYeastMutationM109I (Naturally occurring SNP) and The first 21 …Available SinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRD395
Plasmid#163102PurposeExpression of mCherry_H-NSdbd in Escherichia coli (and, potentially, other bacteria)DepositorInsertmCherry_H-NSdbd
ExpressionBacterialPromoteraraBAD promoterAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
HA-VHL L158S-pRc/CMV
Plasmid#22041DepositorInsertvon Hippel-Lindau tumor suppressor (VHL) (VHL Human)
TagsHA-tagExpressionMammalianMutationpoint mutant converting aa 162 from Cysteine to P…Available SinceSept. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEasyG3_hph
Plasmid#184919PurposeTemplate to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_hph recombines in vivo with a PCR product from pEasyG3_mic.DepositorInsertgRNA scaffold
UseCRISPRExpressionBacterialAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCapV
Plasmid#183028Purposethe patatin-like phosphodiesterase CapV from the animal commensal strain Escherichia coli ECOR31 cloned in the L-arabinose inducible vector pBAD28DepositorInsertpatatin-like phospholipase CapV
TagsNoExpressionBacterialPromoterpBAD promoterAvailable SinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRF+275Dux4
Plasmid#21293DepositorInsertDUX 4 (DUX4 DUX 4, Human)
UseLuciferaseAvailable SinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-pDUXChd:hDUX4ctd-ca
Plasmid#198237PurposeLentivector with tet-inducible porcine DUXC/human DUX4 chimera (codon altered)DepositorInsertDUX4L pDUXChd:hDUX4ctd (codon altered) (DUX4 S. scrofa (porcine), Human)
UseLentiviralExpressionMammalianMutationcodon alteredPromoterTREAvailable SinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 PhiC31 integrase (GB1496)
Plasmid#160578PurposeStreptomyces phage PhiC31 integrase, complete CDSDepositorInsertPhiC31
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
J23117-mRFP1-331Bb
Plasmid#78280PurposemRFP1 behind constitutive promoter J23117 in pSEVA331Bb backboneDepositorInsertJ23117-mRFP1
Available SinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
GST-beta2(616-951)
Plasmid#100743PurposeBacterial expression of GST-tagged beta2 subunit of AP2 complex (hinge and ear) long splice formDepositorAvailable SinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCS2-mqgDUX4
Plasmid#21176DepositorInsertDUX4 (DUX4 Human)
ExpressionBacterial and MammalianAvailable SinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
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pPPAT-11AA-2XStrep
Plasmid#125680Purposeexpresses human PPAT protein, with first 11 residues removed, with a C-terminal 2XStrep affinity tagDepositorInsertPPAT (PPAT Human)
Tags2X Strep-Tag IIExpressionMammalianMutationthe first eleven residues have been removedPromoterCMVAvailable SinceMay 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti GW-mCit-PA
Plasmid#113457PurposeLentiviral ProteinA-mCitrine Gateway shuttle vector for C-terminal fusionsDepositorTypeEmpty backboneUseLentiviralTagsmCitrine-ProteinAExpressionMammalianPromoterhUbCAvailable SinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
HA-IFITM3-F8-TAG-C72A
Plasmid#122477Purposeexpresses human IFITM3-C72A with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.DepositorInsertIFITM3 (IFITM3 Human)
TagsHAExpressionMammalianMutationChanged Phenylalanine 8 to amber codon, cysteine …Available SinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
FB007
Plasmid#119705PurposePromoter gpdA from Aspergillus nidulans domesticated into pUPD2 with GGAG/AATG barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation.DepositorInsertPromoter gpdA
UseSynthetic Biology; Domestication of dna parts for…PromotergpdAAvailable SinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTSlb-Q758C
Plasmid#60729PurposeExpresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations Q758CDepositorInsertsResidues 1-179 of split T7 RNAP
Residues 180-880 of split T7 RNAP
UseSynthetic BiologyExpressionBacterialMutationResidues 1-180 of split T7 RNAP and Residues 180-…Available SinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBullet-pm-c
Plasmid#53078Purposedestination vector with PIP2a-ECFP (plasma membrane marker) for tagged fluorescent protein (C-ter-EYFP) of plant geneDepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
J23114-mRFP1-331Bb
Plasmid#78279PurposemRFP1 behind constitutive promoter J23114 in pSEVA331Bb backboneDepositorInsertJ23114-mRFP1
Available SinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
MBP-GTSE1(long)-His6
Plasmid#69110PurposeFor bacterial expression of recombinant human GTSE1 (longer isoform) tagged with MBP and His.DepositorAvailable SinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti GW-NL-myc
Plasmid#113455PurposeLentiviral NanoLuc Gateway shuttle vector for C-terminal fusionsDepositorTypeEmpty backboneUseLentiviral and LuciferaseTagsNanoLuc-cmycExpressionMammalianPromoterhUbCAvailable SinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCK669
Plasmid#192638PurposeExpresses dxCas9-NG and MCP-SoxS(R93A/S101A) on p15A-CmRDepositorInsertsSp.pCas9-dxCas9-NG
BBa_J23107-MCP-SoxS(R93A, S101A)
UseCRISPR and Synthetic BiologyMutationSoxS has R93A and S101A mutations and dxCas9-NG h…PromoterBBa_J23107 and Sp.pCas9Available SinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEasyG3_zeo
Plasmid#184917PurposeTemplate to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_zeo recombines in vivo with a PCR product from pEasyG3_mic.DepositorInsertgRNA scaffold
UseCRISPRExpressionBacterialAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
FB029
Plasmid#119713PurposePromoter paf from Penicillium chrysogenum domesticated into pUPD2 with GGAG/AATG barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation.DepositorInsertPromoter paf
UseSynthetic Biology; Domestication of dna parts for…PromoterPromoter pafAvailable SinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
FB030
Plasmid#119714PurposeTerminator paf from P. chrysogenum domesticated into pUPD2 with GCTT/CGCT barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformationDepositorInsertTerminator paf
UseSynthetic Biology; Domestication of dna parts for…Available SinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
MSCV N-term HA-FLAG puro BRD7 shRescue
Plasmid#41936DepositorAvailable SinceApril 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET22b-ecDHFR-N23PP/L28F/G51PEKN
Plasmid#118585PurposeBacterial expression of E.coli DHFR N23PP, L28F, and G51PEKN mutantDepositorInsertDHFR
ExpressionBacterialMutationN23 mutated to P23, L28 mutated to F28, G51 mutat…PromoterT7Available SinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT TO myc Separase S1226A/ C2029A
Plasmid#59823PurposeAllows the integration of myc Separase S1226A/ C2029A in the genome and Tet-inducible expression.DepositorInsertSeparase (ESPL1 Human)
TagsMycExpressionMammalianMutationS1226A/ C2029APromoterhybrid human cytomegalovirus (CMV)/TetO2 promoterAvailable SinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only