We narrowed to 17,661 results for: URE

Showing: 10381 - 10410 of 17661 results

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mCherry-YOD1

Plasmid

#85663

Purpose

Expression of human YOD1 with N-terminal mCherry tag

Depositor

Hemmo Meyer

Article

Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

Insert

YOD1 (YOD1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Flag-hsDicer (Y971A/Y972A)

Plasmid

#41590

Depositor

Phil Sharp

Article

Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

Insert

hsDicer (DICER1 Human)

Tags

Flag

Expression

Mammalian

Mutation

Y971A/Y972A

Promoter

CMV

Available Since

Feb. 7, 2013

Availability

Academic Institutions and Nonprofits only
V5-bArr1

Plasmid

#201491

Purpose

Expression of the beta-arrestin1 containing a N-terminal V5 tag.

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

V5-bArr1 (ARRB1 Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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PBLO CasX-gRNA

Plasmid

#126419

Purpose

E. coli vector for CasX gRNA --- Used for In Vitro Transcription

Depositor

Jennifer Doudna

Article

Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

Insert

casX gRNA

Use

CRISPR; In vitro transcription

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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MSCV-miR30_shBrd9_1061-PGK-NeoR-IRES-mCherry

Plasmid

#75132

Purpose

Retroviral expression plasmid encoding shBrd9_1061 (targeting murine Brd9)

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

shBrd9_1061

Use

Retroviral

Available Since

May 23, 2016

Availability

Academic Institutions and Nonprofits only
MK14_HUMAN_D0

Plasmid

#79680

Purpose

This plasmid encodes the kinase domain of MK14. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

MK14

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
FnoCas12a-2C-NLS in pCSDest

Plasmid

#126639

Purpose

Expresses FnoCas12a-2C-NLS in mammalian cells

Depositor

Scot Wolfe

Article

Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

Insert

FnoCas12a-2C-NLS

Use

CRISPR

Tags

3xHuman influenza hemagglutinin (HA)-Tag

Expression

Mammalian

Promoter

CMV IE94 promoter

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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KCC2B_HUMAN_D0

Plasmid

#79718

Purpose

This plasmid encodes the kinase domain of KCC2B. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

KCC2B

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pIN4_tsp-6_mScarlet

Plasmid

#182935

Purpose

Expresses tsp-6::mScarlet under its native promoter (amphid and phasmid ciliated neurons in C. elegans)

Depositor

Maureen Barr

Article

Nikonorova et al Curr Biol. 2022 May 9;32(9):1924-1936.e6

Insert

Ptsp-6-3::tsp-6::flexible linker::mScarlet (C. elegans, genomic)

Tags

flexible linker (GGGGS)x3 and mScarlet tag

Expression

Worm

Promoter

tsp-6

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-MSP1D1deltaH4/2H5

Plasmid

#71715

Purpose

Membrane scaffold protein (MSP), helix 4/2 + helix5 deletion

Depositor

Gerhard Wagner

Article

Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

Insert

Apolipoprotein A1 (APOA1 Human)

Tags

6xHis and TEV site

Expression

Bacterial

Available Since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-Ubl

Plasmid

#78712

Purpose

Expresses Sacsin Ubl- domain (2-85) in fusion with GST (bacterial)

Depositor

Kalle Gehring

Article

Menade et al J Biol Chem. 2018 Aug 17;293(33):12832-1

Insert

Human Sacsin 2-85

Tags

GST-Tag

Expression

Bacterial

Available Since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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Venus-7aa-ActA-pEGFP-C1

Plasmid

#177405

Purpose

To express Venus fused to mitochondria outer membrane targeting sequence "ActA". Venus faces the cytosol.

Depositor

David Andrews

Article

Osterlund et al Protocol Exchange (2022)

Insert

ActA tail anchor (actA Listeria monocytogenes)

Tags

Venus

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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FCK-ss-Prl-Arch-GFP

Plasmid

#22224

Purpose

Lentiviral expression of Arch-GFP, with N-terminal MHC Class I antigen signal sequence (ss) and Prolactin signal sequence (Prl), for optogenetic inhibition.

Depositor

Edward Boyden

Article

Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

Insert

ss-Prl-Arch-GFP

Use

Lentiviral

Tags

GFP, Prl, and ss

Expression

Mammalian

Promoter

CaMKII

Available Since

Jan. 6, 2010

Availability

Academic Institutions and Nonprofits only

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K401-Micro

Plasmid

#122485

Purpose

Component of optically-induced motor dimerization system

Depositor

Matt Thomson

Article

Ross et al Nature. 2019 Aug;572(7768):224-229. doi:

Insert

His tag MBP Kinesin 1-401 SspB micro

Tags

H6-MBP-TEV and SspB micro

Expression

Bacterial

Mutation

Kinesin amino acids 1-401

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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SaBE3-Gam

Plasmid

#100810

Purpose

Expresses SaBE3-Gam in mammalian cells

Depositor

David Liu

Article

Komor et al Sci Adv. 2017 Aug 30;3(8):eaao4774. doi:

Insert

SaBE3-Gam

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 TFAM

Plasmid

#67064

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TFAM (TFAM Human)

Use

Cell-free expression

Tags

EGFP and His

Mutation

S115C

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
T7-p73DD L371P-pcDNA3

Plasmid

#23049

Depositor

William Kaelin

Article

Irwin et al Nature. 2000 Oct 5. 407(6804):645-8.

Insert

tumor protein p73 (TP73 Human)

Tags

T7

Expression

Mammalian

Mutation

truncated cDNA encodes truncated protein (aa 327-…

Available Since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only

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ND4 left TALE–G1333 DddAtox C

Plasmid

#158092

Purpose

expresses the right-side ND4 TALE–DddAtox half in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

SOD2 MTS–3xHA–ND4 left TALE–G1333 DddAtox-C–1xUGI

Expression

Mammalian

Mutation

WT

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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NO- DSD Sensor 3

Plasmid

#199787

Purpose

Synthetic gene circuit

Depositor

Timothy Lu

Article

Inda-Webb et al Nature. 2023 Aug;620(7973):386-392. doi:

Insert

NO- DSD Sensor

Use

Synthetic Biology

Available Since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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mKO-Mito-7

Plasmid

#57848

Purpose

Localization: Mitochondria, Excitation: 548, Emission: 561

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

Mito (COX8A Human)

Tags

mKO

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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LentiGuide-Neo-CTRLg1

Plasmid

#139450

Purpose

Lentiviral vector with non-targeting gRNA and neomycin selectable marker

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

Non-targeting sgRNA inserted; resistance gene: neoR

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a / U6

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_UBASH3B-1/1

Plasmid

#91462

Purpose

Protein expression and purification of human SH3 domain construct UBASH3B-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

UBASH3B-1/1 (UBASH3B Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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Src(WT)-SsrA(Y7I)-mCer3

Plasmid

#220998

Purpose

This is microTag (Y7I mutation in SsrA)-Src with mCerulean3 at C-terminal

Depositor

Klaus Hahn

Article

Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

Insert

Src, SsrA, mCerulean3 (Src Mouse)

Tags

mCerulean3

Expression

Mammalian

Mutation

Y7I in SsrA

Promoter

CMV

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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binder/tag-WT-FAK-insertionB

Plasmid

#190440

Purpose

The tag is SsrA (AANDENY). Tag is inserted after D405 of FAK.

Depositor

Klaus Hahn

Article

Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

Insert

FAK (Ptk2 Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

SsrA inserted after aa 405

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-BBS4

Plasmid

#218713

Purpose

Expresses N-terminally EGFP-tagged BBS4 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

Insert

BBS4 (BBS4 Human)

Tags

EGFP

Expression

Mammalian

Mutation

I354T (t1110c)

Promoter

CMV

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pUBQ10:AtFLS2

Plasmid

#202186

Purpose

Express Arabidopsis thaliana FLS2 gene under UBQ10 promoter

Depositor

Gitta Coaker

Article

Trinh et al Plant Physiol. 2023 May 5:kiad263. doi:

Insert

Flagellin sensing 2 (FLS2 Mustard Weed)

Tags

HA

Expression

Plant

Promoter

AtUBQ10

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-sr1(SIRPT1)

Plasmid

#75297

Purpose

Expresses Sacsin sr1 (SIRPT1 repeat) domain (84-339) in fusion with GST (bacterial)

Depositor

Kalle Gehring

Article

Menade et al J Biol Chem. 2018 Aug 17;293(33):12832-1

Insert

Human Sacsin 84-339

Tags

GST-Tag

Expression

Bacterial

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pKH162_FLAG-APEX2-YIPF3 LIR mut

Plasmid

#214981

Purpose

Flag-APEX2 tagged LIR mutant YIPF3

Depositor

Wade Harper

Article

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

FLAG-APEX2-YIPF3_mut (YIPF3 Human)

Use

Lentiviral

Mutation

LIR mutant

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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tagSrc(WT)-mCer3 S208F/V224L

Plasmid

#179138

Purpose

mCerulean3 has S208F/V224L mutations that promote weak dimerization with YPet to optimize oritentation for FRET. tag is SsrA (AANDENY).

Depositor

Klaus Hahn

Article

Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

Insert

Src, SsrA

Tags

mCerulean3 (S208F/V224L)

Expression

Mammalian

Available Since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pSVL-mo FceRI gamma

Plasmid

#8372

Depositor

Jean Kinet

Article

Ra et al J Biol Chem 1989 Sep 15;264(26):15323-7.

Insert

mouse Fc epsilon RI gamma cDNA (Fcer1g Mouse)

Expression

Mammalian

Available Since

June 7, 2005

Availability

Academic Institutions and Nonprofits only