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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 8,430 results for: BLI

Showing: 6921 - 6940 of 8430 results

  1. pGlomyc-Grhl1 (618 aa)

    Plasmid
    #236049
    Purpose
    Mammalian expression vector with myc-tagged mouse Grhl1 coding sequence (long isoform)
    Depositor
    Renee van Amerongen
    Insert
    Grhl1 (Grhl1 Mouse)
    Use
    Tags
    myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    236049-plasmid-map-sequence-id-474703

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  2. pGlomyc-Grhl2

    Plasmid
    #236050
    Purpose
    Mammalian expression vector with myc-tagged mouse Grhl2 coding sequence
    Depositor
    Renee van Amerongen
    Insert
    Grhl2 (Grhl2 Mouse)
    Use
    Tags
    myc
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    236050-plasmid-map-sequence-id-474702

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  3. pcDNA3.1-IARS2-tb

    Plasmid
    #232340
    Purpose
    Expresses human IARS2 with synonymous mutations (to prevent recognition by siRNA) in mammalian cells
    Depositor
    Quanfu Ma
    Insert
    IARS2 (IARS2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    5 synonymous mutations in the siRNA (ACUUGCAGUCAU…
    Promoter
    CMV
    Available since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    232340-plasmid-map-sequence-id-474527

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  4. pET28a-shortSUMO1

    Plasmid
    #228951
    Purpose
    express a fragment of the human SUMO1 protein (residues 18-97 of UniProt P6316 with an N-terminal thrombin-cleavable tag, MGSSHHHHHHSSGLVPR|GSHM-, where | indicates the cleavage site). Codon-optimized
    Depositor
    Marcin Suskiewicz
    Insert
    SUMO1 (SUMO1 Human)
    Use
    Tags
    His tag and thrombine cleavage site : MGSSHHHHHHS…
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-17
    Promoter
    T7-lac
    Available since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    228951-plasmid-map-sequence-id-472406

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  5. pET28a-RANGAP1-CTD

    Plasmid
    #228947
    Purpose
    express the C-terminal domain of the human RANGAP1 protein (residues 418-587 of UniProt P46060 with the N-terminal thrombin-cleavable MGSSHHHHHHSSGLVPR|GSHM- tag). Codon-optimized
    Depositor
    Marcin Suskiewicz
    Insert
    RANGAP1-CTD (RANGAP1 Human)
    Use
    Tags
    His tag and thrombine cleavage site : MGSSHHHHHHS…
    Expression
    Bacterial
    Mutation
    contains aa 418-587 only
    Promoter
    T7-lac
    Available since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    228947-plasmid-map-sequence-id-472386

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  6. CMV_Mex3a_mutRING_t2a_mCherry

    Plasmid
    #223245
    Purpose
    Expression of mutant RING Mex3a in mammalian cells with t2a cleaved mCherry
    Depositor
    Stavros Lomvardas
    Insert
    Mex3a (Mex3a Mouse)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    4 point mutations in RING Domain: Cys 484 Ala; Hi…
    Promoter
    CMV
    Available since
    May 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    223245-plasmid-map-sequence-id-441774

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  7. pLD-puro-Cc-IFIT5_K197R-VA

    Plasmid
    #191223
    Purpose
    Lentiviral expression of human IFIT5_K197R in mammalian cells
    Depositor
    Mohan Babu
    Insert
    interferon-induced protein with tetratricopeptide repeats 5 (IFIT5 Human)
    Use
    Lentiviral
    Tags
    VA tag
    Expression
    Mammalian
    Mutation
    changed Lysine 197 to Arginine (K197R)
    Promoter
    CMV
    Available since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    191223-plasmid-map-sequence-id-467902

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  8. pLD-puro-Cc-SARS-CoV-2_Spike_Alpha-VA

    Plasmid
    #191217
    Purpose
    Lentiviral expression of SARS-CoV-2 Spike_Alpha in mammalian cells
    Depositor
    Mohan Babu
    Insert
    SPIKE_SARS2_Alpha (S SARS CoV-2)
    Use
    Lentiviral
    Tags
    VA tag
    Expression
    Mammalian
    Mutation
    Del 69-70 Del 144 N501Y A570D D614G P681H T716I S…
    Promoter
    CMV
    Available since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    191217-plasmid-map-sequence-id-422113

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  9. pLD-puro-Cc-SARS-CoV-2_Spike_Beta-VA

    Plasmid
    #191218
    Purpose
    Lentiviral expression of SARS-CoV-2 Spike_Beta in mammalian cells
    Depositor
    Mohan Babu
    Insert
    SPIKE_SARS2_Beta (S SARS CoV-2)
    Use
    Lentiviral
    Tags
    VA tag
    Expression
    Mammalian
    Mutation
    D80A D215G (Del 241-243) K417N E484K N501Y D614G …
    Promoter
    CMV
    Available since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    191218-plasmid-map-sequence-id-422111

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  10. pLD-puro-Cc-SARS-CoV-2_Spike_Delta-VA

    Plasmid
    #191219
    Purpose
    Lentiviral expression of SARS-CoV-2 Spike_Delta in mammalian cells
    Depositor
    Mohan Babu
    Insert
    SPIKE_SARS2_Delta (S SARS CoV-2)
    Use
    Lentiviral
    Tags
    VA tag
    Expression
    Mammalian
    Mutation
    T19R 156del 157del R158G L452R T478K D614G P681R …
    Promoter
    CMV
    Available since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    191219-plasmid-map-sequence-id-422106

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  11. pLD-puro-Cc-SARS-CoV-2_Spike_Lambda-VA

    Plasmid
    #191220
    Purpose
    Lentiviral expression of SARS-CoV-2 Spike_Lambda in mammalian cells
    Depositor
    Mohan Babu
    Insert
    SPIKE_SARS2_Lambda (S SARS CoV-2)
    Use
    Lentiviral
    Tags
    VA tag
    Expression
    Mammalian
    Mutation
    G75V T76I Del 246-252 L452Q F490S D614G T859N
    Promoter
    CMV
    Available since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    191220-plasmid-map-sequence-id-431854

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  12. pLD-puro-Cc-IFIT5_K160R-VA

    Plasmid
    #191222
    Purpose
    Lentiviral expression of human IFIT5_K160R in mammalian cells
    Depositor
    Mohan Babu
    Insert
    interferon-induced protein with tetratricopeptide repeats 5 (IFIT5 Human)
    Use
    Lentiviral
    Tags
    VA tag
    Expression
    Mammalian
    Mutation
    changed Lysine 160 to Arginine (K160R)
    Promoter
    CMV
    Available since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    191222-plasmid-map-sequence-id-467897

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  13. pcDNA3.1-SARS2-Spike-HiBiT

    Plasmid
    #191212
    Purpose
    Transient expression in mammalian cells of SARS-CoV-2 (A1) spike protein tagged on the C-terminus with HiBiT, the high affinity small fragment of split nanoluciferase assay
    Depositor
    Mohan Babu
    Insert
    SARS-CoV-2 spike_WT (S SARS CoV-2)
    Use
    Tags
    HiBiT (high affinity small nanoluciferase fragmen…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    191212-plasmid-map-sequence-id-437009

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  14. pLenti Puro HA-Ubiquitin-K48

    Plasmid
    #236290
    Purpose
    Lentiviral vector expressing HA-tagged ubiquitin K48 mutant
    Depositor
    Addgene Research Program, Melina Fan
    Insert
    Ubiquitin K48 (UBB Human)
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mutation
    Single ubiquitin coding sequence containing K48 m…
    Promoter
    CMV
    Available since
    April 10, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236290-plasmid-map-sequence-id-467837

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  15. pLenti Puro HA-Ubiquitin-K63

    Plasmid
    #236291
    Purpose
    Lentiviral vector expressing HA-tagged ubiquitin K63 mutant
    Depositor
    Addgene Research Program, Melina Fan
    Insert
    Ubiquitin K63 (UBB Human)
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mutation
    Single ubiquitin coding sequence containing K63 m…
    Promoter
    CMV
    Available since
    April 10, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236291-plasmid-map-sequence-id-467835

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  16. pHR_5xUAS-ETV2-P2A-BFP-WPRE_PGK-HygR

    Plasmid
    #216664
    Purpose
    Expressed Gal4-inducible ETV2 and BFP, with hygromycin resistance
    Depositor
    Leonardo Morsut
    Inserts
    ETV2 (Etv2 Mouse)
    BFP
    HygromycinR
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    216664-plasmid-map-sequence-id-432192

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  18. HaloTag-LacI-mHP1a

    Plasmid
    #232637
    Purpose
    lac repressor (LacI) which binds lacO sites; labeled with HaloTag and coupled with effector protein mouse HP1alpha
    Depositor
    Karsten Rippe
    Insert
    HaloTag-LacI-mHP1a (Cbx5 Synthetic, Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    March 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    232637-plasmid-map-sequence-id-463625

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  19. Kif1A-EGFP-SNAP

    Plasmid
    #229851
    Purpose
    Kif1A (aa1-351)-Kif1A neck linker(17aa) fused to Kin1 coiled coil (aa345-406)-EGFP-SNAP-his. This Kif1A was used to link an oligo to the motor using the SNAP tag for single molecule experiments
    Depositor
    William Hancock
    Insert
    Kif1A-EGFP-SNAP (Kif1a Rat)
    Use
    Tags
    6xHis, EGFP, and SNAP-tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    229851-plasmid-map-sequence-id-460711

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  20. Galt1-HT7-mGold

    Plasmid
    #230027
    Purpose
    Expressing fluorescent HaloTag7 fusion protein in mammalian cells with localization in the Golgi apparatus.
    Depositor
    Pablo Rivera-Fuentes
    Insert
    Galt1-HaloTag7-mGold (B4GALT1 Synthetic, Human)
    Use
    Tags
    HaloTag,mGold
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    230027-plasmid-map-sequence-id-458169

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Showing: 6921 - 6940 of 8430 results