We narrowed to 9,391 results for: BLI

1. pAG426-GAL-Alpha-synuclein-H50Q

   Plasmid

   #181724

   Purpose

   Galactose inducible expression of Alpha-synuclein-H50Q

   Depositor

   Alejandro Chavez

   Insert

   Alpha-synuclein (SNCA Human)
   

   Expression

   Yeast

   Mutation

   H50Q

   Available Since

   April 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pScDD2_ERdd

   Plasmid

   #109047

   Purpose

   destabilizing domains for yeast (S. cerevisiae) derived from the human estrogen receptor ligand binding domain. This ERdd can be stabilized with 10 uM 4-hydroxytamoxifen (4-OHT, OHTAM).

   Depositor

   Thomas Wandless

   Insert

   ER50 (ESR1 H. sapiens gene with yeast enhanced codon)
   

   Tags

   yeast enhanced GFP with 8 amino acids TSGRGEGQ li…

   Expression

   Yeast

   Mutation

   3 mutations : E380G, R434G, N532S

   Promoter

   SOD1

   Available Since

   April 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. CD44Z-c011

   Plasmid

   #175455

   Purpose

   Protein expression/secretion in mammalian cells. CD44s (standard form; exons 2-5, 15-16), A20-E649.

   Depositor

   Opher Gileadi

   Insert

   CD44 (CD44 Human)
   

   Tags

   His6

   Expression

   Mammalian

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO.1-blast shKGA

   Plasmid

   #110420

   Purpose

   shKGA, silence glutaminase KGA isoform, blasticidin selection.

   Depositor

   Sandra Martha Gomes Dias

   Insert

   GLS glutaminase (GLS Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   U6 (RNA Pol III)

   Available Since

   June 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. p-dCas9-p300-Hygro

   Plasmid

   #104411

   Purpose

   transient expression of dCas9-p300 fusion protein

   Depositor

   Stephan Beck

   Insert

   p300 (EP300 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTP-AAV-ChATen-mCherry

   Plasmid

   #193741

   Purpose

   This AAV vector contains a ChAT enhancer that drives mCherry expression in spinal cord skeletal motor neurons. Specificity of vector not characterized outside the mouse spinal cord.

   Depositor

   Hynek Wichterle

   Insert

   ChAT enhancer, mini promoter, synthetic intron, mCherry
   

   Use

   AAV and Mouse Targeting

   Expression

   Mammalian

   Promoter

   1000 bp ChAT enhancer with a 32 bp minimal promot…

   Available Since

   Oct. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEC2735 (pSpy0C2)

   Plasmid

   #220279

   Purpose

   integrative plasmid for heterologous gene expression in S. pyogenes SF370, integrates into the amyA locus via homologous recombination

   Depositor

   Emmanuelle Charpentier

   Article

   Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. DOT1L

   Plasmid

   #36196

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   DOT1L (GeneScript Synthesized) (DOT1L Human)
   

   Tags

   His tag with TEV cleavage

   Expression

   Bacterial

   Mutation

   contains amino acid residues 1-420

   Available Since

   Aug. 8, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

9. saposin B^W

   Plasmid

   #161759

   Purpose

   Saposin B (Homo Sapien) containing a single mutation, arginine 38 to tryptophan

   Depositor

   Sam Gellman

   Article

   Kurgan et al Biochemistry. 2021 Apr 13;60(14):1108-11

   Insert

   saposin B R38_W
   

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   changed Arginine 38 to Tryptophan

   Promoter

   T7

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. DOT1L

    Plasmid

    #40736

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    DOT1L (DOT1L Human)
    

    Tags

    6xHis TEV

    Expression

    Bacterial

    Available Since

    Oct. 18, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

11. Hs.KRAS4b G12R

    Plasmid

    #83143

    Purpose

    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12R mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    G12R

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBabe-Kras G12V-T35S

    Plasmid

    #58900

    Purpose

    Expresses human Kras with G12V and T35S mutations

    Depositor

    Channing Der

    Insert

    KRAS (KRAS Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    G12V and T35S mutations (effector binding mutant)

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

13. p413TEF1-ATR1(pYL573)

    Plasmid

    #178288

    Purpose

    Expresses NADPH-cytochrome P450 reductase 1 from A. thaliana (ATR1) in Saccharomyces cerevisiae. Centromeric HIS, PTEF1-ATR1-TCYC1

    Depositor

    Yanran Li

    Article

    Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

    Insert

    ATR1
    

    Expression

    Yeast

    Promoter

    TEF1

    Available Since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-T7-SpCas9-BPNLS(SV40)-3xFLAG-P2A-mScarlet (MNW001)

    Plasmid

    #174091

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a C-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-mScarlet
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-mScarlet

    Expression

    Mammalian

    Promoter

    pCMV and T7

    Available Since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. Hs.KRAS4b S17N

    Plasmid

    #83156

    Purpose

    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), S17N mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    S17N

    Available Since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. ACE2 g4 + Cas9

    Plasmid

    #153014

    Purpose

    A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2

    Depositor

    Jason Sheltzer

    Insert

    Cas9 + ACE2 gRNA (ACE2 Human)
    

    Use

    Lentiviral

    Tags

    TagBFP2

    Available Since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. ERAP1

    Plasmid

    #39174

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    ERAP1 (ERAP1 Human)
    

    Use

    Baculovirus expression

    Tags

    TEV-His10-Flag

    Promoter

    polyhedrin

    Available Since

    June 7, 2013

    Availability

    Academic Institutions and Nonprofits only

18. Hs.KRAS4b Q61L

    Plasmid

    #83134

    Purpose

    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), Q61L mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    Q61L

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pdx1-mTQ2-P2A-FlpO-PA

    Plasmid

    #67279

    Purpose

    Pancreas specific expression of the FlpO recombinase controlled by the pdx1 promoter . FlpO is linked to mTQ2 -a blue fluorescent protein. The gene cassette is in a SB transposon context

    Depositor

    Jannik Elverløv-Jakobsen

    Inserts

    mTQ2=mturquoise2 blue fluorescent gene
    FlpO recombinase
    

    Tags

    linked to FlpO through an P2A ribosomal skipping …

    Expression

    Mammalian

    Promoter

    pdx

    Available Since

    July 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. CasRX_Pac

    Plasmid

    #194803

    Purpose

    CasRX vector for gene knockdown in Giardia. Insert gRNA using Bbs1 restriction site. Puromycin resistance cassette.

    Depositor

    Alexander Paredez

    Article

    Shih et al Proc Natl Acad Sci U S A. 2022 May 31;11

    Insert

    CasRX
    

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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