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pDGE670

Plasmid

#153236

Purpose

Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

Depositor

Sylvestre Marillonnet

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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Orange Nano-lantern/pRSETB

Plasmid

#67659

Purpose

orange luminescent protein for high-speed single-cell and whole body imaging

Depositor

Takeharu Nagai

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Orange Nano-lantern

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+) neo with Ywhag CDS

Plasmid

#61799

Purpose

pcDNA3.1(+) with full length mouse Ywhag CDS

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

Ywhag CDS (Ywhag Mouse)

Expression

Mammalian

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)-ExRai-AKAR(T/A)

Plasmid

#118406

Purpose

Negative-control mutant for ExRai-AKAR biosensor.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

ExRai-AKAR(T/A)

Tags

6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Mutation

Contains Thr-to-Ala mutation in substrate sequenc…

Promoter

CMV

Available Since

April 25, 2019

Availability

Academic Institutions and Nonprofits only

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STAGR_SAMScaffold_mU6

Plasmid

#102845

Purpose

Can be used as PCR template for a STAgR reaction

Depositor

Stefan Stricker

Article

Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

Insert

STAgR Insert SAM_mU6

Use

Pcr template for stagr inserts

Promoter

mU6

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pALS343 (pGEX-2TP-cTevHis-Gibson/MCS)

Plasmid

#139788

Purpose

Vector for recombinant protein expression in E. coli; PreScission-cleavable N-terminal GST and TEV-cleavable C-terminal 6xHis

Depositor

Arneet Saltzman

Article

Saltzman et al Genetics. 2018 Nov;210(3):907-923. doi:

Type

Empty backbone

Tags

6xHis (cleavable by TEV protease) and GST (cleava…

Expression

Bacterial

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-75-2

Plasmid

#73036

Purpose

Express an NT-3-BDNF hybrid neurotrophic factor, with reduced affinity to p75NTR, in mammalian cells

Depositor

Pantelis Tsoulfas

Article

Enomoto et al Exp Neurol. 2013 Oct;248:170-82. doi: 10

Insert

p75-2

Use

Lentiviral

Expression

Mammalian

Mutation

Mature Human NT-3 gene exchanged Aspartic 15 to a…

Available Since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-sapphireAKAR

Plasmid

#118466

Purpose

cp-T-sapphire-based single-color PKA biosensor targeted to the plasma membrane.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

Lyn-sapphireAKAR

Tags

N-terminal targeting sequence from Lyn kinase

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pTU2S-a (colE1 origin)

Plasmid

#74091

Purpose

Secondary module (BpiI) site in pTU2-a backbone with colE1 (medium-copy) origin of replication

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Golden Gate destination vector - Level 2, 2 TU's with secondary module and colE1 origin

Use

Synthetic Biology

Expression

Bacterial

Promoter

None

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pDRf1-4CL5-DsRed (JBEI-15461)

Plasmid

#87935

Purpose

Cloning vector for co-expressing a gene with 4CL5 in S.cerevisiae

Depositor

Dominique Loque

Article

Eudes et al Microb Cell Fact. 2016 Nov 21;15(1):198.

Type

Empty backbone

Use

Synthetic Biology

Expression

Yeast

Available Since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-sapphireAKAR(T/A)

Plasmid

#118465

Purpose

Negative-control mutant for sapphireAKAR biosensor.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

sapphireAKAR(T/A)

Tags

6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Mutation

Contains Thr-to-Ala mutation in substrate sequenc…

Promoter

CMV

Available Since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pDGE652

Plasmid

#153230

Purpose

Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

Depositor

Sylvestre Marillonnet

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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CMVp-ECFP-Triplex-28-8xmiRNA-BS-28-pA (Construct 22)

Plasmid

#55199

Purpose

CFP reporter along with FF4 miRNA binding sites and 28 nt sequences

Depositor

Timothy Lu

Article

Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

Insert

ECFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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RFN_EGFP_81

Plasmid

#60073

Purpose

Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease

Depositor

Keith Joung

Article

Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

Insert

pair of multiplex gRNAs targeting EGFP

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Oct. 16, 2014

Availability

Academic Institutions and Nonprofits only
pENTR221-PGK.Pro

Plasmid

#79489

Purpose

MultiSite Gateway entry clones, first fragment, (attL1, attR5) Human PGK promoter

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

PGK Promoter (PGK1 Human, Synthetic)

Use

Multisite gateway entry clone

Promoter

PGK

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR221-SMAD3

Plasmid

#79507

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human SMAD3 CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

SMAD3 (SMAD3 Human)

Use

Multisite gateway entry clone

Tags

FLAG

Promoter

no Promoter

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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Cyan Nano-lantern/pRSETB

Plasmid

#67658

Purpose

cyan luminescent protein for high-speed single-cell and whole body imaging

Depositor

Takeharu Nagai

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Cyan Nano-lantern

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only
Cyan Nano-lantern(Ca2+)/pcDNA3

Plasmid

#71344

Purpose

Express Cyan Nano-lantern-based luminescent Ca2+ indicator in mammalian cell

Depositor

Takeharu Nagai

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Cyan Nano-lantern(Ca2+)

Expression

Mammalian

Available Since

Dec. 7, 2015

Availability

Academic Institutions and Nonprofits only
pBS U6 WASp RNAi

Plasmid

#11775

Depositor

Jack Strominger

Article

Krzewski et al J Cell Biol. 2006 Apr 10. 173(1):121-32.

Insert

WASp RNAi (WAS Human)

Use

RNAi

Expression

Mammalian

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pAAV-SMVP-Cas9N-U6-gRNA TdL

Plasmid

#80938

Purpose

Expresses Cas9N in mammalian cells; expresses gRNA TdL for Ai9 cleavage.

Depositor

George Church

Article

Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

Insert

Cas9N

Use

AAV, CRISPR, and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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