We narrowed to 58,550 results for: tra

1. pXD70Tet(DadHR)-DadR-Pdadh-DadhABC(A2)

   Plasmid

   #191645

   Purpose

   E. coli - Eggerthella lenta shuttle plasmid (TetR), native promoter-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strain with transcriptional regulator DadR

   Depositor

   Emily Balskus

   Article

   Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

   Insert

   Dopamine dehydroxylase
   

   Expression

   Bacterial

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTV-Flnc-5prime-1000-24MS96T-3F_NeoR

   Plasmid

   #174881

   Purpose

   Targeting vector for generating Flnc STREAMING-tag KI cell

   Depositor

   Hiroshi Ochiai

   Article

   Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

   Insert

   STREAMING-tag with homology arms for Flnc
   

   Use

   Mouse Targeting

   Available Since

   Dec. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTV-Usp5-5prime-1000-24MS96T-3F_NeoR

   Plasmid

   #174885

   Purpose

   Targeting vector for generating Usp5 STREAMING-tag KI cell

   Depositor

   Hiroshi Ochiai

   Article

   Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

   Insert

   STREAMING-tag with homology arms for Usp5
   

   Use

   Mouse Targeting

   Available Since

   Dec. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTV-Wnk1-5prime-1000-24MS96T-3F_NeoR

   Plasmid

   #174886

   Purpose

   Targeting vector for generating Wnk1 STREAMING-tag KI cell

   Depositor

   Hiroshi Ochiai

   Article

   Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

   Insert

   STREAMING-tag with homology arms for Wnk1
   

   Use

   Mouse Targeting

   Available Since

   Dec. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTSK405

   Plasmid

   #190817

   Purpose

   For C-terminus 3xGFP tagging of a gene of interest using URA3 selection marker in S. cerevisiae

   Depositor

   Tatiana Karpova, Gunjan Mehta

   Article

   Ball et al Nucleic Acids Res. 2016 Dec 1;44(21):e16

   Insert

   URA3 (URA3 Budding Yeast)
   

   Use

   Bacterial cloning vector

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIN2_mcm-3_mScarlet

   Plasmid

   #182933

   Purpose

   Expresses mcm-3 under its native promoter (embryonic nuclei and ciliated neurons IL2, CEMs, HOB, RnBs in C. elegans)

   Depositor

   Maureen Barr

   Article

   Nikonorova et al Curr Biol. 2022 May 9;32(9):1924-1936.e6

   Insert

   genomic mcm-3 fused to mScarlet
   

   Tags

   flexible linker (GGGGS)x3 and mScarlet tag

   Expression

   Worm

   Promoter

   mcm-3

   Available Since

   Aug. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. psiCheck2-SV40p-mtIF3(SR)-3xFLAG-HSVTKp-mCherry

   Plasmid

   #182381

   Purpose

   Dual expression construct encoding shRNA-resistant mtIF3 and mCherry from separate promoters

   Depositor

   Chunghun Lim

   Article

   Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

   Inserts

   mtIF3 (Mtif3 Mouse)
   mCherry
   

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Changed CDS sequence from 'ccacgttcaagtcacga…

   Promoter

   HSV TK promoter and SV40 promoter

   Available Since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEPQD1CB0867

   Plasmid

   #177085

   Purpose

   MoClo Level 1, position 3, transcriptional unit for transient expression of 7-deoxyloganetic acid glucosyl transferase (Cr7-DLGT) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31

   Depositor

   Nicola Patron

   Article

   Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

   Insert

   7-deoxyloganetic acid glucosyl transferase (Cr7-DLGT) from Catharanthus roseus
   

   Use

   Synthetic Biology

   Available Since

   Dec. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEPQD1CB0806

   Plasmid

   #177080

   Purpose

   MoClo Level 1, position 3, transcriptional unit for transient expression of loganic acid O-methyltransferase (CrLAMT) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31

   Depositor

   Nicola Patron

   Article

   Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

   Insert

   loganic acid O-methyltransferase (CrLAMT) from Catharanthus roseus
   

   Use

   Synthetic Biology

   Available Since

   Dec. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEPQD1CB0861

    Plasmid

    #177078

    Purpose

    MoClo Level 1, position 6, transcriptional unit for transient expression of 7-deoxyloganetic acid glucosyl transferase (Cr7-DLGT) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    7-deoxyloganetic acid glucosyl transferase (Cr7-DLGT) from Catharanthus roseus
    

    Use

    Synthetic Biology

    Available Since

    Dec. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1 MYC dBR

    Plasmid

    #176046

    Purpose

    Expresses Myc Mutant (delta basic region) in Mammalian cells

    Depositor

    Linda Penn

    Article

    Tu et al Cancer Cell. 2018 Oct 8;34(4):579-595.e8

    Insert

    Myc
    

    Expression

    Mammalian

    Mutation

    deleted amino acids 355-369

    Promoter

    CMV

    Available Since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pIRES-EGFP-TPD53

    Plasmid

    #172461

    Purpose

    Expression of untagged Tumor Protein D52 like 1 (TPD53/TPD52L1) and GFP

    Depositor

    Stephen Royle

    Article

    Larocque et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

    Insert

    TPD53 (TPD52L1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pIRES-EGFP-TPD54

    Plasmid

    #172459

    Purpose

    Expression of untagged Tumor Protein D52 like 2 (TPD54/TPD52L2) and GFP

    Depositor

    Stephen Royle

    Article

    Larocque et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

    Insert

    TPD54 (TPD52L2 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMS19: pHelper(PmcCAST)_PmcPSP1_ΔTniQ

    Plasmid

    #168152

    Purpose

    Inducible expression of PmcCAST proteins (except TniQ) with crRNA targeting PmcPSP1.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Inserts

    PmcCAST minimal CRISPR array (with spacer for PmcPSP1)
    PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
    PmcCAST Tns proteins (TnsAB, TnsC and TnsD)
    

    Expression

    Bacterial

    Available Since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMS2: pHelper(AvCAST)_AvPSP1_ΔTniQ

    Plasmid

    #168134

    Purpose

    Inducible expression of AvCAST proteins (except TniQ) with crRNA targeting AvPSP1.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Inserts

    AvCAST minimal CRISPR array (with spacer for AvPSP1)
    AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
    AvCAST Tns proteins (TnsA, TnsB and TnsC)
    AvTnsD
    

    Expression

    Bacterial

    Available Since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCFJ2308 - [1-2] ENTR - Fluor - mNeon(1 intron, syntrons(3), NLS(2), no_atg, no_stop)

    Plasmid

    #159852

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - mNeon(1 intron, syntrons(3), NLS(2), no_atg, no_stop)
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSEM266 - pEXP(mosTI, hygroR)[Pmex-5 | ceGFP(PATC, NLS) | tbb-2 3'UTR]

    Plasmid

    #159829

    Purpose

    Single copy or array insertion by MosTI using split hygroR selection

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    pEXP(mosTI, hygroR)[Pmex-5 | ceGFP(PATC, NLS) | tbb-2 3'UTR]
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSEM267 - pEXP(mosTI, neoR)[Pmex-5 | ceGFP(PATC, NLS) | tbb-2 3'UTR]

    Plasmid

    #159833

    Purpose

    Single copy or array insertion by MosTI using split NeoR selection

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    pEXP(mosTI, neoR)[Pmex-5 | ceGFP(PATC, NLS) | tbb-2 3'UTR]
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPRIME-CMV-GFP-shNts2

    Plasmid

    #132714

    Purpose

    Encodes short hairpin RNA (shRNA) #2 that targets the 3’-untranslated region of the rat Nts

    Depositor

    Robert Messing

    Article

    Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

    Insert

    shNts(2) (Nts Rat)
    

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    Oct. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPRIME-CMV-GFP-shNts1

    Plasmid

    #132713

    Purpose

    Encodes short hairpin RNA (shRNA) #1 that targets the the 3’-untranslated region of the rat Nts gene

    Depositor

    Robert Messing

    Article

    Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

    Insert

    shNts(1) (Nts Rat)
    

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    Oct. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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