160,922 results

1. pAAV-EWB-DIO-cyan pre-eGRASP(p32)

   Plasmid

   #111589

   Purpose

   An AAV vector that expresses double floxed cyan pre-eGRASP(p32) under the Ef1a promoter.

   Depositor

   Bong-Kiun Kaang

   Article

   Choi et al Science. 2018 Apr 27;360(6387):430-435.

   Insert

   cyan pre-eGRASP(p32)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Ef1a

   Available since

   June 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR223-PIK3R1

   Plasmid

   #23696

   Depositor

   David Root, William Hahn

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   PIK3R1 (PIK3R1 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pLL5.0 E-cadherin shRNA/E-cadherin GFP-IRES-HA-H-Ras V12

   Plasmid

   #101285

   Purpose

   Lentiviral expression ,was generatedby recombining bothIRES an HA-tagged H-Ras V12 into SbfI site of pLL5.0 Ecadherin shRNA/mEcad-GFP

   Depositor

   Alpha Yap

   Article

   Wu et al Nat Cell Biol. 2014 Feb;16(2):167-78. do

   Insert

   E-Cadherin-GFP-H-Ras
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   H-Ras V12

   Promoter

   Available since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC19-P70a-3WJdB-T

   Plasmid

   #87311

   Purpose

   Expresses the fluorescence-activating Three-way Junction dimeric Broccoli (3WJdB) aptamer under the control of the E. coli sigma 70 promoter.

   Depositor

   Donald Burke

   Article

   Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

   Insert

   Three-way Junction dimeric Broccoli aptamer
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   P70a

   Available since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR_Gal4UAS_4D5-High-CAR-mCherry_PGK_4D5-Low-SynNotch_Gal4VP64_RHL133

   Plasmid

   #164822

   Purpose

   Lentiviral vector for Gal4 inducible AntiHER2 4D5-High CAR-mCherry and constitutive expression of antiHER2 4D5-Low Gal4VP64 Synthetic Notch

   Depositor

   Wendell Lim

   Article

   Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

   Inserts

   AntiHER2 4D5-High Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)
   AntiHER2 4D5-Low Gal4VP64 synthetic Notch receptor
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   miniCMV and pGK

   Available since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-Flag-YAP-5SA/S94A

   Plasmid

   #33103

   Depositor

   Kunliang Guan

   Article

   Zhao et al Genes Dev. 2008 Jul 15. 22(14):1962-71.

   Insert

   YAP (YAP1 )
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S61A, S94A, S109A, S127A, S164A, S381A

   Promoter

   Available since

   Feb. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. Kras-Src FRET biosensor

   Plasmid

   #78302

   Purpose

   FRET biosensor. To monitor Src kinase activity in mammalian cells by FRET

   Depositor

   Yingxiao Wang

   Article

   Ouyang et al Proc Natl Acad Sci U S A. 2008 Sep 23;10

   Insert

   Src biosensor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEF-BFP

   Plasmid

   #138272

   Purpose

   Expression of engineered BFP variant that undergoes conversion to GFP after targeted modification with a cytidine deaminase-based DNA base editor

   Depositor

   Xiao Wang

   Article

   Standage-Beier et al Nucleic Acids Res. 2019 Aug 20. pii: 555

   Insert

   BFP H66 PAM
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   H66, PAM motif

   Promoter

   Ef1a

   Available since

   March 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR_PGK_SNIPR_hsNotch1 LNR

   Plasmid

   #188336

   Purpose

   Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a Notch1-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

   Depositor

   Kole Roybal

   Article

   Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

   Insert

   PGK_antiCD19_Notch1-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV.hSyn.FLP-FRT.iGluSnFR3.v857.GPI

    Plasmid

    #196223

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Insert

    pAAV hSyn FLP-FRT iGluSnFR3 v857.GPI
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    GPI anchor, IgK-chain, and Myc epi tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-hCas9D10A

    Plasmid

    #52101

    Purpose

    The expression vector for humanized nickase Cas9 (Cas9D10A) under CAG promoter

    Depositor

    Izuho Hatada

    Insert

    hCas9D10A
    

    Use

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    CAG

    Available since

    April 30, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pLenti-trGluc

    Plasmid

    #158959

    Purpose

    DNA donor plasmid containing repair template for Gaussia luciferase (Gluc) of bioluminescent repair reporter (BLRR).

    Depositor

    Charles P. Lai

    Article

    Chien et al Nucleic Acids Res. 2020 Aug 14. pii: 589

    Insert

    trGluc
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBMN mCherry-OPTN(D474N)

    Plasmid

    #119683

    Purpose

    For mammalian expression of UBD mutant OPTN

    Depositor

    Michael Lazarou

    Article

    Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

    Insert

    OPTN (OPTN Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    D474N

    Promoter

    Available since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET His6 GST TEV LIC cloning vector (2G-T)

    Plasmid

    #29707

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    6xHis, GST, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMW41: pET-His-MBP-SUMO-R2Bm-SII

    Plasmid

    #209290

    Purpose

    Expresses the R2Bm ORF in E. coli

    Depositor

    Feng Zhang

    Article

    Wilkinson et al Science. 2023 Apr 21;380(6642):301-308.

    Insert

    R2
    

    Use

    Tags

    His, MBP, StrepII, and bdSUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. VEGFR2-mCh

    Plasmid

    #108854

    Purpose

    Encodes for human VEGFR2 fluorescently labeled with mCherry on the C-Terminus via a 3 amino acid (GGS) flexible linker

    Depositor

    Kalina Hristova

    Article

    Sarabipour et al Elife. 2016 Apr 7;5:e13876. doi: 10.7554

    Insert

    KDR (KDR Human)
    

    Use

    Tags

    Labeled with mCherry on the C-Terminus via a 3 am…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pmScarlet-i_H2A_C1

    Plasmid

    #85053

    Purpose

    In vivo visualization of the nucleus (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    H2A (H2AC16 Human)
    

    Use

    Tags

    mScarlet-i

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. hCMV-IE1:Renilla

    Plasmid

    #118066

    Purpose

    Transcriptional unit for constitutive expression of Renilla in mammalian cells

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Insert

    CMV:GreenRenilla:bGHF
    

    Use

    Luciferase and Synthetic Biology; Assembly of gb2…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMXs-IP HA-Parkin

    Plasmid

    #38248

    Depositor

    Noboru Mizushima

    Article

    Yoshii et al J Biol Chem. 2011 Jun 3;286(22):19630-40

    Insert

    Parkin (PRKN Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mCerulean3-mVenus-CASPER

    Plasmid

    #58162

    Purpose

    Localization: Cytosolic FRET, Excitation: , Emission:

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    mCerulean3 and mVenus-CASPER

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV-TPH2-Cre

    Plasmid

    #189616

    Purpose

    Expresses Cre recombinase under the control of the tryptophan hydroxylase promoter

    Depositor

    David Clapham

    Article

    Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.

    Insert

    Cre Recombinase
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tph2

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRecT_2

    Plasmid

    #167546

    Purpose

    Encodes an IPTG inducible recT from E. faecium Com15 used to ectopically express recT in Enterococcus for recombineering.

    Depositor

    Howard Hang

    Article

    Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00

    Insert

    E. faecium Com15 recT under an IPTG inducible promoter, with lacI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    IPTG

    Available since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pENN226_CRISPRcharm

    Plasmid

    #220834

    Purpose

    CRISPRcharm expression in mammalian cells

    Depositor

    Jonathan Weissman

    Article

    Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

    Insert

    CRISPRcharm
    

    Use

    CRISPR

    Tags

    P2A-TagBFP

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CAG

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. TFORF2027

    Plasmid

    #143994

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NPAS2 (NPAS2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCap-SG5-Hgy

    Plasmid

    #227580

    Purpose

    Capture plasmid with hygromycin resistance and pSG5 replicon

    Depositor

    Chengzhang Fu

    Article

    Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLH-sgRNA1-2XPP7

    Plasmid

    #75390

    Purpose

    sgRNA1-2XPP7

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

    Insert

    sgRNA1-2XPP7
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. BPK1520_puroR

    Plasmid

    #173901

    Purpose

    SpCas9 guide cloning vector derived from BPK1520 that allows for puromycin-based selection

    Depositor

    Ronald Cohn

    Article

    Erwood et al Genome Res. 2019 Dec;29(12):2010-2019. d

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/U6

    Available since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. TVT7R(0,0)

    Plasmid

    #98631

    Purpose

    Vector for cloning infectious RNA virus genomes

    Depositor

    Andrew Ball, Karyn Johnson

    Article

    Johnson et al J Virol. 2000 Jun;74(11):5123-32.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3β-FLAG-CBP-HA

    Plasmid

    #32908

    Depositor

    Tso-Pang Yao

    Article

    Zhao et al Mol Cell Biol. 2005 Oct;25(19):8456-64.

    Insert

    CBP (Crebbp Mouse)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pTECH-chAcK3RS(IPYE)

    Plasmid

    #104069

    Purpose

    Expression plasmid encoding chAcK3RS(IPYE) and pylT

    Depositor

    David Liu

    Article

    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

    Insert

    Plpp chAcK3RS(IPYE); PproK pyl
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Contains activating mutations V31I, T56P, H62Y, A…

    Promoter

    Plpp and PproK

    Available since

    Dec. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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