We narrowed to 2,056 results for: ENDOG

1. pRGE32

   Plasmid

   #63159

   Purpose

   Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for transient expression in protoplast

   Depositor

   Yinong Yang

   Article

   Xie et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Promoter

   Rice snoRNA U3 promoter for gRNA/PTG expression a…

   Available Since

   April 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMC_mNG2(11)_BSDminus_F2

   Plasmid

   #184164

   Purpose

   Donor cassette plasmid for high-throughput tagging, encoding an mNG2(11) synthetic exon in frame 2, as well as a blasticidin resistance gene for selection of genomic integrants.

   Depositor

   Ophir Shalem

   Article

   Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

   Inserts

   mNG2(11)
   bsd
   

   Use

   CRISPR

   Available Since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-TadA8e-Ns3h

   Plasmid

   #221227

   Purpose

   Express HACE Editor as a fusion of TadA-8e adenosine deaminase and Ns3h helicase.

   Depositor

   Fei Chen

   Article

   Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

   Insert

   TadA8e-Ns3h
   

   Expression

   Mammalian

   Available Since

   June 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX330-PITCh-H2BC11

   Plasmid

   #207755

   Purpose

   Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of H2BC11 for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting C-terminus of H2BC11 (H2BC11 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Dec. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. qTAG-AAVS1-PGK-Blast

   Plasmid

   #227270

   Purpose

   Empty AAVS1 targeting donor for the insertion of Blast and a medium strength PGK promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-TadA8e-PcrA

   Plasmid

   #221228

   Purpose

   Express HACE Editor as a fusion of TadA-8e adenosine deaminase and PcrA helicase.

   Depositor

   Fei Chen

   Article

   Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

   Insert

   TadA8e-PcrA
   

   Expression

   Mammalian

   Available Since

   June 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330_DRS-1 sgRNA / hSpCas9

   Plasmid

   #172841

   Purpose

   Mammalian expression of the DRS-1 synthetic sgRNA sequence under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   DRS-1 sgRNA under the U6 promotor and hSpCas9 under the CAG promotor
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-CibN-dCas9-CibN

   Plasmid

   #60553

   Purpose

   Expresses CibN-dCas9-CibN in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

   Insert

   dCas9-CibN
   

   Use

   CRISPR

   Tags

   FLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   inactivating Cas9 mutations D10A and H840A

   Promoter

   CMV

   Available Since

   Feb. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEMHE-mEGFP-mTrim21

   Plasmid

   #105519

   Purpose

   T7 promotor drives in vitro transcription of mEGFP-tagged wildtype mouse TRIM21 mRNA

   Depositor

   Melina Schuh

   Article

   Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

   Insert

   TRIM21 (Trim21 Mouse)
   

   Use

   In vitro transcription

   Tags

   mEGFP

   Promoter

   T7

   Available Since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC57-Amp_CRISPIE donor B1

    Plasmid

    #172842

    Purpose

    CRISPIE donor B1 (Zhong et al, eLife 2021), mEGFP translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    CRISPIE designer exon (phase 0-0) encoding mEGFP
    

    Use

    CRISPR

    Available Since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX459V2.0-HypaCas9-mRuby2

    Plasmid

    #183872

    Purpose

    pX459V2.0 plasmid expressing HypaCas9 with P2A-mRuby2 and T2A-Puro, with a sgRNA cloning site for CRISPR editing in mammalian cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    HypaCas9-P2A-mRuby2-T2A-Puro

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV.hSyn.Cre

    Plasmid

    #170367

    Purpose

    AAV vector expressing Cre under human synapsin promotor

    Depositor

    Johan Jakobsson

    Article

    Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

    Insert

    Cre
    

    Use

    AAV

    Promoter

    human Synapsin

    Available Since

    Jan. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. hDHFR

    Plasmid

    #184707

    Purpose

    bacterial expression of human DHFR

    Depositor

    Peter Wright

    Article

    Bhabha et al FEBS Lett. 2011 Nov 16;585(22):3528-32.

    Insert

    DHFR (DHFR Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330-PITCh-PXN

    Plasmid

    #227315

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of PXN for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of PXN (PXN Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV.U6gLacZ.hSyn.flex.H2B.RFP

    Plasmid

    #170373

    Purpose

    Negative control guide, Cre-inducible plasmid expressing nuclear RFP.

    Depositor

    Johan Jakobsson

    Article

    Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

    Insert

    Cre inducible H2B RFP
    

    Use

    AAV

    Promoter

    human Synapsin

    Available Since

    Jan. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAGGS-mEGFP-loxP-P2A-dTKneo-pA-loxP

    Plasmid

    #139484

    Purpose

    PCR template for mEGFP gene knock-in donor vector

    Depositor

    Kazuhiro Aoki

    Article

    Komatsubara et al J Biol Chem. 2019 Apr 12;294(15):6062-60

    Insert

    mEGFP-loxP-P2A-dTKneo-pA-loxP
    

    Tags

    mEGFP

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    April 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a-His6-ODC-Ctag

    Plasmid

    #163614

    Purpose

    Expresses truncated human Ornithine Decarboxylase in bacterial cells for binding to Ornithine Decarboxylase Antizyme.

    Depositor

    Mark Howarth

    Article

    Scheu et al Nat Commun. 2021 Jan 29;12(1):717. doi:

    Insert

    ODC (ODC1 Human)
    

    Tags

    C-tag and His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUC57-Amp_CRISPIE donor B2

    Plasmid

    #172843

    Purpose

    CRISPIE donor B2 (Zhong et al, eLife 2021), mRuby3 translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 or DRS-2 sgRNA (with SpCas9).

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    CRISPIE designer exon (phase 0-0) encoding mRuby3
    

    Use

    CRISPR

    Available Since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBS-KS-attB1-2-PT-SA-SD-1-GFP11

    Plasmid

    #172068

    Purpose

    Protein trap plasmid for inserting GFP11 into a phase 1 coding intron

    Depositor

    Daichi Kamiyama

    Article

    Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

    Insert

    GFP11x7
    

    Expression

    Insect

    Available Since

    Oct. 20, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. ULK1-Halo HRD

    Plasmid

    #207548

    Purpose

    Homologous recombination donor to integrate a 3xFLAG-HaloTag at the C-terminus of the endogenous ULK1 locus in human cells

    Depositor

    Jens Schmidt

    Article

    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

    Insert

    HaloTag followed by a PolyA signal and PuroR cassette flanked by human ULK1 locus sequences
    

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    Endogenous

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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