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CRISPR-StAR2 (Retro)

Plasmid

#222688

Purpose

CRISPR-screening

Depositor

Ulrich Elling

Article

Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

Type

Empty backbone

Use

CRISPR and Retroviral

Tags

Expression

Mammalian

Mutation

PGK without BbsI cutsite

Promoter

Available since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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CRISPR-StAR3 (Retro)

Plasmid

#222689

Purpose

CRISPR-screening

Depositor

Ulrich Elling

Article

Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

Type

Empty backbone

Use

CRISPR and Retroviral

Tags

Expression

Mammalian

Mutation

PGK without BbsI cutsite

Promoter

Available since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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CRISPR-StAR4 (Retro)

Plasmid

#222690

Purpose

CRISPR-screening

Depositor

Ulrich Elling

Article

Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

Type

Empty backbone

Use

CRISPR and Retroviral

Tags

Expression

Mammalian

Mutation

PGK without BbsI cutsite

Promoter

Available since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-fDIO-VARNAM2-Kv2.1PR

Plasmid

#195536

Purpose

Flp recombinase-dependent red fluorescent, negative response-polarity voltage indicator; soma-targeted

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

VARNAM2-Kv2.1 proximal restriction sequence

Use

AAV

Tags

Expression

Mammalian

Mutation

VARNAM SI linker

Promoter

CAG

Available since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-NRF3

Plasmid

#207815

Purpose

N-terminal tagged NRF3 for miniTurbo proximity experiments

Depositor

Ben Major

Article

LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

Insert

miniTurbo-V5-NRF3 (NFE2L3 Human)

Use

Lentiviral

Tags

miniTurbo-V5

Expression

Mutation

Promoter

UBC

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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p667-UBC-NRF3-V5-miniTurbo

Plasmid

#207816

Purpose

C-terminal tagged NRF3 for miniTurbo proximity experiments

Depositor

Ben Major

Article

LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

Insert

NRF3-V5-miniTurbo (NFE2L3 Human)

Use

Lentiviral

Tags

V5-miniTurbo

Expression

Mutation

Promoter

UBC

Available since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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p207-Switch-ON (FRT)

Plasmid

#217886

Purpose

Retroviral Switch-ON vector for sgRNA expression; U6-BbsIx2-SWITCH-ON-scaffold; neoR

Depositor

Ulrich Elling

Article

Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

Type

Empty backbone

Use

CRISPR and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pECIA14

Plasmid

#47051

Purpose

This is a Gateway destination vector for secreted expression in Drosophila cell culture by CuSO4 induction. Inserts will be pentamerized and tagged with Alkaline phosphatase, FLAG and His tags

Depositor

Chris Garcia

Article

Ozkan et al Cell. 2013 Jul 3;154(1):228-39. doi: 10.

Type

Empty backbone

Use

Secreted expression in drosophila culture

Tags

Alkaline Phosphatase (human, placental), Flag, HR…

Expression

Insect

Mutation

Promoter

Metallothionein (Copper-Inducible)

Available since

Aug. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-VARNAM2-Kv2.1PR

Plasmid

#195524

Purpose

Red fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter; soma-targeted

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

VARNAM2-Kv2.1 proximal restriction sequence

Use

AAV

Tags

Expression

Mammalian

Mutation

VARNAM SI linker

Promoter

Synapsin

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-VARNAM2-Kv2.1PR

Plasmid

#195530

Purpose

Red fluorescent, negative response-polarity voltage indicator under the control of CaMKII promoter; soma-targeted

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

VARNAM2-Kv2.1 proximal restriction sequence

Use

AAV

Tags

Expression

Mammalian

Mutation

VARNAM SI linker

Promoter

CaMKII

Available since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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p206-Switch-ON

Plasmid

#217885

Purpose

Retroviral Switch-ON vector for sgRNA expression; U6-BbsIx2-SWITCH-ON-scaffold; neoR

Depositor

Ulrich Elling

Article

Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-WT (AF41)

Plasmid

#21367

Depositor

Gregory Germino

Article

Qian et al Proc Natl Acad Sci U S A. 2002 Dec 24. 9

Insert

autosomal dominant polycystic kidney disease type I (PKD1 Human)

Use

Tags

EGFP and FLAG

Expression

Mammalian

Mutation

wild-type sequence*

Promoter

Available since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pQTEV-HSPB8

Plasmid

#34710

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

Insert

heat shock 22 kDa protein 8 (HSPB8 Human)

Use

Tags

His and TEV

Expression

Bacterial

Mutation

Promoter

Available since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-pAceR

Plasmid

#195521

Purpose

Red fluorescent, positive response-polarity voltage indicator under the control of synapsin promoter

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

pAceR

Use

AAV

Tags

Expression

Mammalian

Mutation

VARNAM 78E, 81D, 92N; SI linker

Promoter

Synapsin

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pQStrep2-ARPC3

Plasmid

#31580

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

Insert

Actin related protein 2/3 complex, subunit 3 (ARPC3 Human)

Use

Tags

His and StrepII-tag

Expression

Bacterial

Mutation

Promoter

Available since

March 25, 2013

Availability

Academic Institutions and Nonprofits only

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pLC-EGFP-RIP3

Plasmid

#75165

Purpose

LentiCRISPR-EGFP with sgRNA targeting human RIP3

Depositor

Beat Bornhauser

Article

McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

Insert

RIP3 sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pLC-mCherry-MLKL

Plasmid

#75167

Purpose

LentiCRISPR-mCherry with sgRNA targeting human MLKL

Depositor

Beat Bornhauser

Article

McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

Insert

MLKL sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pQTEV-MAP1LC3A

Plasmid

#34824

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

Insert

microtubule-associated protein 1 light chain 3 alpha (MAP1LC3A Human)

Use

Tags

His and TEV

Expression

Bacterial

Mutation

Promoter

Available since

March 25, 2013

Availability

Academic Institutions and Nonprofits only

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phFluc-WT

Plasmid

#177714

Purpose

Cytosolic firefly luciferase reporter previously described in Shcherbakov et al. 2019

Depositor

Erik Böttger

Article

Shcherbakov et al Commun Biol. 2019 Oct 17;2:381. doi: 10.

Insert

hFluc WT

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Jan. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pAD-CMV-STAT5B-DN-CMV-GFP

Plasmid

#83262

Purpose

Adenoviral expression of dominant negative STAT5B with co-expression of GFP

Depositor

Andrew Brooks

Article

Legrand et al J Invest Dermatol. 2016 Sep;136(9):1781-

Inserts

Stat5b (Stat5b Mouse)
eGFP

Use

Adenoviral

Tags

FLAG

Expression

Mutation

dominant negative (Y699F)

Promoter

CMV

Available since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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