Skip to main content
Addgene
Showing: 1361 - 1400 of 1645 results

  4. pCDH-EF1-E-NoMi-P2A-CopGFP-T2A-PuroR

    Plasmid
    #207805
    Purpose
    E-NoMi is a re-engineered tetraspanin scaffold tagged with bioluminescent and fluorescent reporter proteins under an EF-1α promoter.
    Depositor
    Koen Breyne
    Article
    Maalouf et al Mol Ther. 2023 Jul 5;31(7):2206-2219. do
    Insert
    E-NoMi
    Use
    Lentiviral
    Tags
    3xFlag tag, copGFP, mCherry, and nanoluciferase
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1alpha
    Available since
    Jan. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    207805-plasmid-map-sequence-id-419158

    Enlarge

  11. AICSDP-63: ACTN2-mEGFP

    Plasmid
    #124607
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human ACTN2, via excisable Cas9-excisable CAGGS-mCherry selection cassette
    Depositor
    Allen Institute for Cell Science
    Insert
    ACTN2 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (ACTN2 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    124607-plasmid-map-sequence-id-244336

    Enlarge

  18. pBLU-VIPR

    Plasmid
    #220498
    Purpose
    Plasmid allowing for potent induction of gRNA using blue light. The plasmid contains VPR-EL222 and the C120 response element followed by mCherry and cloning sites for ribozyme flanked gRNA (RGR).
    Depositor
    Alexander Espinosa
    Article
    Pulgarin et al bioRxiv [Preprint]. 2023 Nov 10:2023.11.
    Type
    Empty backbone
    Use
    CRISPR; Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220498-plasmid-map-sequence-id-442816

    Enlarge

  21. pDONOR 6colors

    Plasmid
    #229974
    Purpose
    bigMamAct assembled plasmid for 6colors cellular labelling
    Depositor
    Martin Pelosse
    Article
    Pelosse et al Synthetic Biology 2025.02.13
    Insert
    mito-mCherry, EYFP-tubulin, H2B iRFP720, GTS-mTagBFP, mTFP1-actin
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    See Depositor Comments
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    229974-plasmid-map-sequence-id-461259

    Enlarge

  22. Equalizer-L episome

    Plasmid
    #169738
    Purpose
    Equalizer-L on an episomal vector. This plasmid encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.
    Depositor
    Francois St-Pierre
    Article
    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:
    Insert
    miR(FF4) target-tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pCMV-tetO2
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    169738-plasmid-map-sequence-id-334158

    Enlarge

  25. jk107

    Plasmid
    #87007
    Purpose
    plasmid for cell culture expression of SYNseq components
    Depositor
    Anthony Zador
    Article
    Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11
    Inserts
    SNAP-Nlg1AB-1xλN(i)
    mCherry
    Use
    Synthetic Biology; Sindbis virus genomic plasmid
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    87007-plasmid-map-sequence-id-173032

    Enlarge

  30. AICSDP-55:DMD-mEGFP

    Plasmid
    #159744
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human DMD via Cas9-excisable CAGGS-mCherry selection cassette
    Depositor
    Allen Institute for Cell Science
    Insert
    DMD Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (DMD Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    159744-plasmid-map-sequence-id-314374

    Enlarge

  34. jk64

    Plasmid
    #87012
    Purpose
    genomic Sindbis virus plasmid for use in SYNseq PLA screen
    Depositor
    Anthony Zador
    Article
    Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11
    Inserts
    Nrx1B-4xλN(i)
    mCherry
    Use
    Synthetic Biology; Sindbis virus genomic plasmid
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    87012-plasmid-map-sequence-id-173035

    Enlarge

  37. IDP429

    Plasmid
    #87016
    Purpose
    genomic Sindbis virus plasmid for use in SYNseq PLA screen
    Depositor
    Anthony Zador
    Article
    Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11
    Inserts
    CLIP-Nrx1B-GFP-1xλN(c)
    mCherry
    Use
    Synthetic Biology; Sindbis virus genomic plasmid
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    87016-plasmid-map-sequence-id-169748

    Enlarge

  40. jk68

    Plasmid
    #87020
    Purpose
    genomic Sindbis virus plasmid for use in SYNseq PLA screen
    Depositor
    Anthony Zador
    Article
    Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11
    Inserts
    SNAP-Nlg1AB-4xλN(i)
    mCherry
    Use
    Synthetic Biology; Sindbis virus genomic plasmid
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    87020-plasmid-map-sequence-id-173038

    Enlarge

Showing: 1361 - 1400 of 1645 results