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We narrowed to 5,231 results for: codon optimized

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Showing: 1701 - 1750 of 5231 results

  1. pHN1002

    Plasmid
    #178831
    Purpose
    Bacterial expression for human Saposin A
    Depositor
    Kazuhiro Abe
    Insert
    artificial gene encoding human Saposin A (AR Human)
    Tags
    His8, 3C
    Expression
    Bacterial
    Mutation
    Codon-usage was optimized for bacterial expressio…
    Promoter
    T7
    Available Since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    178831-plasmid-map-sequence-id-351933

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  3. pHH0103 YAP1 WW domain #2

    Plasmid
    #104228
    Purpose
    Bacterial expression of WW domain #2 from YAP1
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    YAP1 WW domain #2 (YAP1 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    104228-plasmid-map-sequence-id-204296

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  4. pHH0103 YAP1 WW domain #1

    Plasmid
    #104227
    Purpose
    Bacterial expression of WW domain #1 from YAP1
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    YAP1 WW domain #1 (YAP1 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    104227-plasmid-map-sequence-id-201508

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  7. NXPE1_pcDNA3.4-TOPO

    Plasmid
    #226841
    Purpose
    Expresses NXPE1 protein residues 60-547 in mammalian cells (HEK293T or expi293). x6 His tag and IL2 secretion signal included N terminal with GGGG linker to protein sequence.
    Depositor
    Bert Vogelstein
    Insert
    NXPE1 (NXPE1 Human)
    Tags
    x6 HIS and an IL2 secretion signal both N termina…
    Expression
    Mammalian
    Mutation
    Codon optimized for expression in human cells
    Available Since
    Dec. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    226841-plasmid-map-sequence-id-456206

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  8. pLV-BDNF-pHluorin

    Plasmid
    #187193
    Purpose
    To express the mouse BDNF tagged with the FP pHluorin
    Depositor
    Pantelis Tsoulfas
    Insert
    BDNF (Bdnf Mouse)
    Use
    Lentiviral
    Tags
    pPHluorin
    Expression
    Mammalian
    Mutation
    The codon were optimized for better expression
    Promoter
    CMV
    Available Since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    187193-plasmid-map-sequence-id-373713

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  9. pIK86

    Plasmid
    #47933
    Purpose
    C. elegans codon-optimized Cas9 with 2xSV40 NLS sequences. It has a T7 promoter for in vitro transcription, germline-compatible 5'UTR, tbb-2 3'UTR and a polyA sequence.
    Depositor
    Iskra Katic
    Article
    Katic et al Genetics. 2013 Aug 26.
    Insert
    Cas9
    Use
    CRISPR and Unspecified
    Promoter
    T7
    Available Since
    Oct. 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    47933-plasmid-map-sequence-id-68745

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  11. DYRK1A

    Plasmid
    #38913
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    DYRK1A (DYRK1A Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  12. HSD11B1

    Plasmid
    #39155
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    HSD11B1 (HSD11B1 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    T220I; codon-optimized
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  13. pISV-CRISPR/Cas9PGmUbi

    Plasmid
    #209189
    Purpose
    Expression of Cas9 in the model legume Medicago truncatula, Gmubi promoter (PGmubi), DsRed and Basta selection
    Depositor
    Christian Staehelin
    Inserts
    Cas9
    DsRed(deltaB)
    Tags
    NLS
    Expression
    Plant
    Mutation
    missing BsaI site and plant-codon optimized with …
    Promoter
    CaMV 35S and GmUbi from Glycine max (L.) Merr. cv…
    Available Since
    Jan. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    209189-plasmid-map-sequence-id-419145

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  14. pU6-Universal

    Plasmid
    #52694
    Purpose
    This plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.
    Depositor
    Sebastian Lourido
    Inserts
    Cas9
    Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
    Use
    CRISPR; Toxoplasma gondii
    Tags
    3X FLAG and Nuclear localization signal
    Promoter
    TgTUB1 and Toxoplasma U6
    Available Since
    June 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    52694-plasmid-map-sequence-id-82051

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  16. pV1025

    Plasmid
    #111424
    Purpose
    Expression of Candida albicans codon-optimized Cas9 (CaCas9) - integrates at ENO1 (Part of Duet system)
    Depositor
    Gerald Fink
    Article
    Vyas et al Sci Adv. 2015;1(3):e1500248.
    Insert
    CaCas9
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    Oct. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    111424-plasmid-map-sequence-id-212243

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  17. ACACA

    Plasmid
    #39083
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ACACA (ACACA Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  22. SpCas9 iFE

    Plasmid
    #190138
    Purpose
    Expresses SpCas9-POLB (iFE)
    Depositor
    Timothy Lu
    Article
    Nakade et al bioRxiv 2022.12.05.518807
    Insert
    SpCas9-POLB (HiFi iFE)
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Codon Optimized for mammalian cells
    Promoter
    CBh
    Available Since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    190138-plasmid-map-sequence-id-374863

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  23. pGBW-m4133639

    Plasmid
    #152344
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 3C-like proteinase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;3xFlag
    Expression
    Mammalian
    Mutation
    codon optimized
    Promoter
    CMV
    Available Since
    June 17, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152344-plasmid-map-sequence-id-300328

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  24. HPGD

    Plasmid
    #39154
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    HPGD (HPGD Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  26. pHN873

    Plasmid
    #178829
    Purpose
    Bacterial expression for human Saposin A
    Depositor
    Kazuhiro Abe
    Insert
    artificial gene encoding human Saposin A (AR Human)
    Tags
    MBP, His8, TEV
    Expression
    Bacterial
    Mutation
    Codon-usage was optimized for bacterial expressio…
    Promoter
    T7
    Available Since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    178829-plasmid-map-sequence-id-351932

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  27. pKG113

    Plasmid
    #63073
    Purpose
    Retroviral expression of EGFP-TEV-S-tag CENP-T for protein localization and affinity purification (LAP)
    Depositor
    Iain Cheeseman
    Insert
    CENP-T (CENPT Human)
    Use
    Retroviral
    Tags
    EGFP and TEV-S
    Expression
    Mammalian
    Mutation
    E. coli codon optimized. G217R mutation compared …
    Available Since
    Jan. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    63073-plasmid-map-sequence-id-134725

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  30. pGBW-m4133731

    Plasmid
    #152742
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 3C-like proteinase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;3xFlag
    Expression
    Mammalian
    Mutation
    codon optimized
    Promoter
    CMV
    Available Since
    July 30, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152742-plasmid-map-sequence-id-313507

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  31. pGBW-m4133568

    Plasmid
    #152340
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 3C-like proteinase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;V5
    Expression
    Mammalian
    Mutation
    codon optimized
    Promoter
    CMV
    Available Since
    July 30, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152340-plasmid-map-sequence-id-420347

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  32. LZ3 iFE

    Plasmid
    #190143
    Purpose
    Expresses LZ3Cas9-POLB (LZ3 iFE)
    Depositor
    Timothy Lu
    Article
    Nakade et al bioRxiv 2022.12.05.518807
    Insert
    LZ3Cas9-POLB (LZ3_iFE)
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Codon Optimized for mammalian cells
    Available Since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    190143-plasmid-map-sequence-id-374873

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  34. SpCas9 dFE

    Plasmid
    #190137
    Purpose
    Expresses SpCas9-T4pol (dFE)
    Depositor
    Timothy Lu
    Article
    Nakade et al bioRxiv 2022.12.05.518807
    Insert
    SpCas9-T4pol (dFE)
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Codon Optimized for mammalian cells
    Promoter
    CBh
    Available Since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    190137-plasmid-map-sequence-id-382840

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  35. pGBW-m4133256

    Plasmid
    #152133
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 3C-like proteinase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHis-003
    Expression
    Mammalian
    Mutation
    codon optimized
    Promoter
    CMV
    Available Since
    May 20, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152133-plasmid-map-sequence-id-300772

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  36. pGBW-m4133586

    Plasmid
    #152328
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 3C-like proteinase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHis-003
    Expression
    Mammalian
    Mutation
    codon optimized
    Promoter
    CMV
    Available Since
    July 30, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152328-plasmid-map-sequence-id-342371

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  37. pGBW-m4133862

    Plasmid
    #152487
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 3C-like proteinase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;HA
    Expression
    Mammalian
    Mutation
    codon optimized
    Promoter
    CMV
    Available Since
    July 30, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152487-plasmid-map-sequence-id-317734

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  38. pGBW-m4133872

    Plasmid
    #152464
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 3C-like proteinase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;3xFlag
    Expression
    Mammalian
    Mutation
    codon optimized
    Promoter
    CMV
    Available Since
    July 30, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152464-plasmid-map-sequence-id-309465

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  39. pHH0103 KIBRA WW domain #2

    Plasmid
    #104230
    Purpose
    Bacterial expression of WW domain #2 from KIBRA
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    KIBRA WW domain #2 (WWC1 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    104230-plasmid-map-sequence-id-204290

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  40. GUCY1B3

    Plasmid
    #39035
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    GUCY1B3 (GUCY1B1 Human)
    Tags
    TEV-His6-Flag
    Expression
    Bacterial
    Mutation
    codon-optimized
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  41. HSD17B14

    Plasmid
    #39160
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    HSD17B14 (HSD17B14 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  42. pCR241

    Plasmid
    #240644
    Purpose
    Expression of AmNeonGreen-13myc in Exaiptasia diaphana; In vitro transcription of the same construct via SP6 polymerase
    Depositor
    John Pringle
    Inserts
    AIPGENE865 promoter
    SP6 promoter
    AmNeonGreen
    Use
    Gene expression and genomic integration in fish
    Tags
    13myc
    Mutation
    Codon-optimized for Exaiptasia diaphana, amino ac…
    Available Since
    Aug. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    240644-plasmid-map-sequence-id-482908

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  43. pHR_SFFV*/Puro2a-PAG1-GFP

    Plasmid
    #198223
    Purpose
    Lentiviral plasmid derived from pHR_SFFV (Addgene #79121) expressing cDNA for a codon-optimized puromycin resistance gene, followed by an FMDV 2A domain and then EGFP
    Depositor
    Eric Davis
    Insert
    PAG1 (PAG1 Human)
    Use
    Lentiviral
    Tags
    Followed by EGFP, after a flexible linker and Pre…
    Expression
    Mammalian
    Mutation
    None
    Available Since
    April 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    198223-plasmid-map-sequence-id-389384

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  44. LZ3 dFE

    Plasmid
    #190144
    Purpose
    Expresses LZ3Cas9-T4pol (LZ3 dFE)
    Depositor
    Timothy Lu
    Article
    Nakade et al bioRxiv 2022.12.05.518807
    Insert
    LZ3Cas9-T4pol (LZ3 dFE)
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Codon Optimized for mammalian cells
    Available Since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    190144-plasmid-map-sequence-id-376723

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  45. HiFi dFE

    Plasmid
    #190142
    Purpose
    Expresses HiFiCas9-T4pol (HiFi dFE)
    Depositor
    Timothy Lu
    Article
    Nakade et al bioRxiv 2022.12.05.518807
    Insert
    HiFi Cas9-T4pol (HiFi dFE)
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    SpCas9 R691A mutant, Codon Optimized for mammalia…
    Promoter
    CBh
    Available Since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    190142-plasmid-map-sequence-id-374983

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  48. pGBW-m4134186

    Plasmid
    #152962
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 3C-like proteinase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;TapTag
    Expression
    Mammalian
    Mutation
    codon optimized
    Promoter
    CMV
    Available Since
    July 30, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152962-plasmid-map-sequence-id-295974

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  49. pGBW-m4133251

    Plasmid
    #152818
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 3C-like proteinase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;HA
    Expression
    Mammalian
    Mutation
    codon optimized
    Promoter
    CMV
    Available Since
    July 30, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152818-plasmid-map-sequence-id-295830

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  50. pGBW-m4133367

    Plasmid
    #152791
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 3C-like proteinase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;S-tag
    Expression
    Mammalian
    Mutation
    codon optimized
    Promoter
    CMV
    Available Since
    July 30, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152791-plasmid-map-sequence-id-295803

    Enlarge

Showing: 1701 - 1750 of 5231 results