We narrowed to 10,893 results for: ENA;
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Plasmid#130821PurposeExpresses LifeAct-iFAST in mammalian cells (actin labeling)DepositorInsertLifeAct-iFAST
UseTagsmyc-tagExpressionMammalianMutationPromoterCMVAvailable SinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAG498 MAP4-iFAST
Plasmid#130820PurposeExpresses MAP4-iFAST in mammalian cells (microtubule labeling)DepositorInsertMAP4-iFAST
UseTagsMyc-tagExpressionMammalianMutationPromoterCMVAvailable SinceOct. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
C-His-GvpC-TEV
Plasmid#153296PurposeExpresses TEV-sensitive variant of Anabaena flos-aquae GvpC in E.coliDepositorInsertTEV-sensitive Gas vesicle protein C (his-tagged)
UseTagsHis-tagExpressionBacterialMutation1) 21 bp TEV recognition and cleavage site flanke…PromoterT7Available SinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
N-His-GvpC-ssrA
Plasmid#153297PurposeExpresses ClpXP-degradable variant of Anabaena flos-aquae GvpC in E.coliDepositorInsertGas vesicle protein C (with N-terminal his-tag and C-terminal ssrA tag)
UseTagsHis-tag and ssrA tagExpressionBacterialMutation1) N-terminal 6xHis tag flanked by 1x glycine res…PromoterT7Available SinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPKm-232
Plasmid#90501PurposepSIN - EF-1alpha - MTS tHO1 - P2A - MTS - tPCYA, vector encoding for mitochondrial-tagged Thermosynechococcus elongates Heme Oxigenase-1 (HO1) and Phycocyanobilin:ferredoxin oxireductase, under EF-1alpha promoterDepositorInsertmito-tHO1 and mito-tPCYA
UseLentiviralTagsExpressionMutationPromoterAvailable SinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPKm-234
Plasmid#90507PurposepSIN - EF-1alpha - MTS sHO1 - P2A - MTS - sPCYA, vector encoding for mitochondrial-tagged Synechococcus sp. Heme Oxigenase-1 (HO1) and Phycocyanobilin:ferredoxin oxireductase (PcyA), under EF-1alpha promoterDepositorInsertmito-sHO1, mito-sPCYA
UseLentiviralTagsExpressionMutationPromoterAvailable SinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1 nptII-DISTC02-CBS-FRT-Rep-RepA-tNos-DISTC19-CBS-miniDFR-FLP-tNos-PROXC02-pNos-CUP2-Gal4AD-tNos-SF (GB4697)
Plasmid#225440PurposeModule for copper and flippase-regulated expression of BeYDV Rep/RepA, including the TU for copper-regulated FLP, the TU for constitutive expression of CUP2-GAL4, and the nptII cassette.DepositorInsertnptII-DISTC02-CBS-FRT:Rep/RepA:tNos-DISTC19-CBS:miniDFR:FLP:tNos-PROXC02-pNos:CUP2:Gal4AD:tNos-SF
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable SinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCOA-Leu-FvPPT1
Plasmid#179923PurposeExpression vector for heterologous expression of a polyketide synthase in S. cerevisiae. Carries and empty insertion site and the Fusarium verticillioides 4´-phosphopantetheinyltransferase PPT1DepositorInsertFvPPT1
UseTagsExpressionYeastMutationCodon optimized for expression in Saccharomyces c…PromoterYarrowia lipolytica EXP1Available SinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSEVA251_ Ptrc.x.tetO2::D-HicDH
Plasmid#185456PurposeCDS of D-HicDH codon optimized for expression in Synechocystis sp. PCC 6803 under the control of the synthetic promoter Ptrc.x.tetO2 and the RBS BBa_B0030 in pSEVA251 plasmid.DepositorInsertD-HicDH from Lactobacillus paracasei DSM 20008
UseTagsExpressionBacterialMutationPromoterPtrc.x.tetO2Available SinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAG660 lyn11-pFAST
Plasmid#172863Purposeexpresses lyn11-pFAST in mammalian cells (inner membrane labeling)DepositorInsertLyn11-pFAST
UseTagsMyc tag (C terminal on insert)ExpressionMammalianMutationPromoterCMVAvailable SinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFB-U6-Chrnb4-gRNA-hSyn-mCherry-WPRE-SV40pA
Plasmid#128345PurposepAAV encoding gRNA sequence for loss-of-function indelsDepositorAvailable SinceJuly 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDF1-ADAMTS18 (P1035S)
Plasmid#29558DepositorInsertADAMTS18 (ADAMTS18 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationchanged proline 1035 to serinePromoterAvailable SinceSept. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCDF1-ADAMTS18 (C638S)
Plasmid#29555DepositorInsertADAMTS18 (ADAMTS18 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationchanged cysteine 638 to serinePromoterAvailable SinceJuly 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pPKm-240
Plasmid#90510PurposepSIN - EF-1alpha - Cyto -sFd - P2A - Cyto - sFNR, vector encoding for cytoplasmic-tagged Synechococcus sp. Heme Oxigenase-1 (HO1) and Phycocyanobilin:ferredoxin oxireductase (PcyA), under EF-1alpha promoterDepositorInsertcyto-sFD, cyto-sFNR
UseLentiviralTagsExpressionMutationPromoterAvailable SinceAvailabilityAcademic Institutions and Nonprofits only -
pHR EGFR-CD3ε-FusionRed
Plasmid#188626PurposeHuman EGFR labeled with CD3ε ITAMs for monitoring with CD3ε/ZtSH2 pYtag biosensorDepositorInsertEGFR (EGFR Human, Synthetic)
UseLentiviralTagsCD3ε-FusionRedExpressionMammalianMutationPromoterSFFVAvailable SinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-TetOn-ZIM3-KRAB-dCas9-P2A-mCherry
Plasmid#212829PurposeDoxycycline-inducible (Zim3)KRAB-dCas9-HA-P2A-mCherry cassette for integration at the AAVS1-locus of the human genome.DepositorInsertZIM3-KRAB-dCas9-P2A-mCherry
UseTagsHA-tag and ZIM3-KRABExpressionBacterial and MammalianMutationPromoterCAG, TetOnAvailable SinceJune 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
mARGAna (cassette 1, transient)
Plasmid#197588PurposeSecond-generation mammalian acoustic reporter gene derived from AnabaenaDepositorInsertmARGAna (cassette 1)
UseTagsExpressionMammalianMutationPromoterAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO/GFPZNF598-9P-9A
Plasmid#141192PurposeExpresses GFP-ZNF598 mutated in polyproline streches in mammalian cells, can be used to make inducible cell lineDepositorInsertZNF598 (ZNF598 Human)
UseTagsGFPExpressionMammalianMutationall 3 proline repeats are mutated to Alanine (9xP…PromoterCMVAvailable SinceJune 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
SpySwitch
Plasmid#184225PurposeExpresses SpySwitch in the bacterial cytoplasm. SpySwitch binds SpyTag-, SpyTag002- or SpyTag003-fusions non-covalently for affinity purification, allowing gentle pH or temperature release.DepositorInsertSpySwitch
UseTagsHis6ExpressionBacterialMutationE77A mutation prevents isopeptide bond formation,…PromoterT7Available SinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDH-cGAS-E225A D227A-mCherry2-EF1-Puro
Plasmid#132771PurposeInteracts with cytoplasmic DNA. Useful for identifying sites of nuclear envelope rupture.DepositorInsertCyclic GMP-AMP synthase (CGAS Human)
UseLentiviralTagsmCherry2ExpressionMammalianMutationE225A D227APromoterAvailable SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only