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pAM-DCA-HA-Ast-3-NP-1-IRES-mCherry-WPRE

Plasmid

#159630

Purpose

AAV expression of HA Allatostatin-3, neurophysin, IRES mCherry under the CAG promoter

Depositor

Ross Bathgate, Andrew Allen

Article

Ngo et al Cell Rep. 2020 Sep 15;32(11):108139. doi

Insert

Ast (AstA Fly)

Use

AAV

Tags

HA, IRES, mCherry, and neurophysin-1

Expression

Mammalian

Mutation

Promoter

DCA (same as CAG promoter)

Available since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-C-6His-mEsrra

Plasmid

#173152

Purpose

mouse estrogen-related receptor alpha (Esrra) expression plasmid in mammalian cells

Depositor

Brijesh Kumar Singh

Article

Tripathi et al bioRxiv 2021.11.16.468891

Insert

ORF clone Estrogen-related receptor alpha (Esrra) (Esrra Mouse)

Use

Tags

His

Expression

Mammalian

Mutation

Wild type

Promoter

Available since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pCSC-CMV-HA-Ast-3-NP-1-IRES-mCherry

Plasmid

#159631

Purpose

expresses HA Allatostatin-3, Neurophysin-1, IRES mCherry under the CSC promoter

Depositor

Ross Bathgate, Andrew Allen

Article

Ngo et al Cell Rep. 2020 Sep 15;32(11):108139. doi

Insert

Ast (AstA Fly)

Use

Lentiviral

Tags

HA, IRES, mCherry, and neurophysin-1

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pMXs-IRES-blast GFP-SPNS1

Plasmid

#198571

Purpose

SPNS1 codon-optimized orf with n-terminal 3xFlag GFP in pMXs-IRES-Blast vector

Depositor

Monther Abu-Remaileh

Article

Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

Insert

SPNS1 with n-terminal 3xFlag-GFP fusion (SPNS1 Human)

Use

Retroviral

Tags

3xFlag-GFP

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pMXs-3xFlag-SPNS1

Plasmid

#198568

Purpose

SPNS1 codon-optimized orf with n-terminal 3xFlag in pMXs-IRES-Blast vector

Depositor

Monther Abu-Remaileh

Article

Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

Insert

SPNS1 with n-terminal 3xFlag (SPNS1 Human)

Use

Retroviral

Tags

3xFlag

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-EWSR1 No Stop

Plasmid

#84888

Purpose

DONOR vector for Gateway cloning of EWSR1 No Stop

Depositor

Aaron Gitler

Article

Couthouis et al Hum Mol Genet. 2012 Jul 1;21(13):2899-91

Insert

EWSR1 (EWSR1 Human)

Use

Gateway donor

Tags

Expression

Mutation

no stop

Promoter

none

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only
pDONR221-EWSR1 Stop

Plasmid

#84887

Purpose

DONOR vector for Gateway cloning of EWSR1

Depositor

Aaron Gitler

Article

Couthouis et al Hum Mol Genet. 2012 Jul 1;21(13):2899-91

Insert

EWSR1 (EWSR1 Human)

Use

Gateway donor

Tags

Expression

Mutation

Promoter

none

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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R989-X05-636: His6-MBP-tev-G-Hs.KRAS4b(1-188)

Plasmid

#159553

Purpose

Baculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188)

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-G-Hs.KRAS4b(1-188) (KRAS Human)

Use

Tags

His6-MBP-tev

Expression

Insect

Mutation

Promoter

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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R989-X02-566: His6-MBP-tev-G-Hs.KRAS4b(1-169)

Plasmid

#159539

Purpose

E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169)

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-G-Hs.KRAS4b(1-169) (KRAS Human)

Use

Tags

His6-MBP-tev

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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R989-X05-566: His6-MBP-tev-G-Hs.KRAS4b(1-188)

Plasmid

#159546

Purpose

E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188)

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-G-Hs.KRAS4b(1-188) (KRAS Human)

Use

Tags

His6-MBP-tev

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR221-EWSR1 g1750a No Stop

Plasmid

#84894

Purpose

DONOR vector for Gateway cloning of EWSR1 g1750a No Stop

Depositor

Aaron Gitler

Article

Couthouis et al Hum Mol Genet. 2012 Jul 1;21(13):2899-91

Insert

EWSR1 (EWSR1 Human)

Use

Gateway donor

Tags

Expression

Mutation

g1750a No Stop

Promoter

none

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only
pDONR221-EWSR1 g1750a Stop

Plasmid

#84893

Purpose

DONOR vector for Gateway cloning of EWSR1 g1750a Stop

Depositor

Aaron Gitler

Article

Couthouis et al Hum Mol Genet. 2012 Jul 1;21(13):2899-91

Insert

EWSR1 (EWSR1 Human)

Use

Gateway donor

Tags

Expression

Mutation

g1750a

Promoter

none

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only
pDONR221-EWSR1 c1655t No Stop

Plasmid

#84892

Purpose

DONOR vector for Gateway cloning of EWSR1 c1655t No Stop

Depositor

Aaron Gitler

Article

Couthouis et al Hum Mol Genet. 2012 Jul 1;21(13):2899-91

Insert

EWSR1 (EWSR1 Human)

Use

Gateway donor

Tags

Expression

Mutation

c1655t No Stop

Promoter

none

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only
pDONR221-EWSR1 c1655t Stop

Plasmid

#84891

Purpose

DONOR vector for Gateway cloning of EWSR1 c1655t Stop

Depositor

Aaron Gitler

Article

Couthouis et al Hum Mol Genet. 2012 Jul 1;21(13):2899-91

Insert

EWSR1 (EWSR1 Human)

Use

Gateway donor

Tags

Expression

Mutation

c1655t

Promoter

none

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only
pDONR221-EWSR1 g1532c No Stop

Plasmid

#84890

Purpose

DONOR vector for Gateway cloning of EWSR1 g1532c No Stop

Depositor

Aaron Gitler

Article

Couthouis et al Hum Mol Genet. 2012 Jul 1;21(13):2899-91

Insert

EWSR1 (EWSR1 Human)

Use

Gateway donor

Tags

Expression

Mutation

g1532c No Stop

Promoter

none

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only
R701-X54-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12D

Plasmid

#159562

Purpose

E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12D

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12D (KRAS Human)

Use

Tags

His6-MBP-tev-Avi

Expression

Bacterial

Mutation

G12D

Promoter

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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R701-X21-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G12D

Plasmid

#159569

Purpose

E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G12D

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G12D (KRAS Human)

Use

Tags

His6-MBP-tev-Avi

Expression

Bacterial

Mutation

G12D

Promoter

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pLV[TetOn]-Puro-TRE3G>mAscl1

Plasmid

#198755

Purpose

Vector encodes the gene for murine Ascl1 under control of a Tet-controlled promoter and the puromycin resistance gene under control of a constitutive PGK promoter

Depositor

Nael Nadif Kasri

Article

Mossink et al Mol Psychiatry. 2022 Jan;27(1):1-18. doi

Insert

Ascl1 (Ascl1 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

TRE3G

Available since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pLV[TetOn]-Puro-TRE3G>mNeurog2

Plasmid

#198754

Purpose

Vector encodes the gene for murine neurogenin-2 under control of a Tet-controlled promoter and the puromycin resistance gene under control of a constitutive PGK promoter

Depositor

Nael Nadif Kasri

Article

Mossink et al Mol Psychiatry. 2022 Jan;27(1):1-18. doi

Insert

Neurogenin-2 (Neurog2 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

TRE3G

Available since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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Human SHIP2

Plasmid

#124645

Purpose

Expresses HIS tagged human SHIP2 in COS-7/HEK cells

Depositor

Christophe Erneux

Article

Pesesse et al J Biol Chem. 2001 Jul 27;276(30):28348-5

Insert

INPPL1 (INPPL1 Human)

Use

Tags

His Xpress (N-terminal)

Expression

Mammalian

Mutation

coding sequence starts at amino acid 18 as calcul…

Promoter

Available since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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