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pShuttle-CMV-iRFP

Plasmid

#31856

Purpose

a phytochrome-based near-infrared fluorescent protein, also known as iRFP713, used for in vivo imaging

Depositor

Vladislav Verkhusha

Article

Filonov et al Nat Biotechnol. 2011 Jul 17. doi: 10.103

Insert

iRFP

Use

Adenoviral

Expression

Mammalian

Mutation

T2A, S13L, A92T, V104I, V114I, E161K, Y193K, F198…

Promoter

CMV

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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pFRT/TO/HIS/FLAG/HA-ALKBH5

Plasmid

#38073

Depositor

Markus Landthaler

Article

Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

Insert

ALKBH5 alkB, alkylation repair homolog 5 (E. coli) (ALKBH5 Human)

Tags

HIS/FLAG/HA

Expression

Mammalian

Mutation

cDNA clone MGC:71143 IMAGE:6339184 with longer 3&…

Available Since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only

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Vinculin-venus

Plasmid

#27300

Depositor

Martin Schwartz

Article

Grashoff et al Nature. 2010 Jul 8. 466(7303):263-6.

Insert

Vinculin (VCL Chicken)

Tags

Venus A206K

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 3, 2011

Availability

Academic Institutions and Nonprofits only

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pCAG-scFvGCN4sfGFPTET1CD

Plasmid

#82561

Purpose

Expression vector for antibody-sfGFP-TET1CD.

Depositor

Izuho Hatada

Article

Morita et al Nat Biotechnol. 2016 Aug 29. doi: 10.103

Insert

scFvGCN4sfGFPTET1CD

Expression

Mammalian

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pHAGE NFKB-TA-LUC-UBC-dTomato-W

Plasmid

#49335

Purpose

pHAGE NFKB-TA-LUC-UBC-dTomato-W

Depositor

Darrell Kotton

Article

Wilson et al Mol Ther. 2013 Apr;21(4):825-33. doi: 10

Inserts

NFkB consensus binding sequence
TA
Luciferase
UBC
dTomato

Use

Lentiviral

Available Since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-dCas9

Plasmid

#112233

Purpose

Lentiviral plasmid for expression of gRNA and dCas9 in mammalian cells; derivative of lentiCRISPR v2

Depositor

Thomas Gilmore

Article

Babaei et al PLoS One. 2019 Sep 25;14(9):e0222588. do

Inserts

dCas9
Porumycin resistance

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Changed: Aspartic acid 10 to Glycine, Histidine 8…

Promoter

EFS-NS

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pMKO.1 puro hTERT shRNA

Plasmid

#10688

Depositor

William Hahn

Article

Masutomi et al Proc Natl Acad Sci U S A. 2005 Jun 7. 10

Insert

hTERT shRNA (TERT Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Jan. 6, 2006

Availability

Academic Institutions and Nonprofits only

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903 pLNCX HA Akt1

Plasmid

#9004

Depositor

William Sellers

Article

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

Akt1 (AKT1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-EGFP

Plasmid

#89875

Purpose

EGFP reporter vector of iTango system for AAV production

Depositor

Hyungbae Kwon

Article

Lee et al Nat Methods. 2017 May;14(5):495-503. doi

Insert

tetO-EGFP

Use

AAV

Expression

Mammalian

Promoter

tetO

Available Since

May 26, 2017

Availability

Academic Institutions and Nonprofits only

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pQC membrane TdTomato IX

Plasmid

#37351

Depositor

Connie Cepko

Article

Beier et al Dev Biol. 2011 May 15;353(2):309-20. Epu

Insert

membrane TdTomato

Tags

Palmitoylation signal*

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

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pMIEG3-JunDN

Plasmid

#40350

Depositor

Alexander Dent

Article

Wang et al J Immunol. 2005 Feb 15;174(4):2098-105.

Insert

Jun dominant negative (JUN Human)

Use

Retroviral

Tags

HA and IRES eGFP

Expression

Mammalian

Mutation

first 122 amino acids deleted

Available Since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV6M-Pak1 K299R

Plasmid

#12210

Depositor

Jonathan Chernoff

Article

Sells et al Curr Biol. 1997 Mar 1. 7(3):202-10.

Insert

Pak1 (PAK1 Human)

Tags

Myc

Expression

Mammalian

Mutation

Catalytically inactive Pak1: K299R

Available Since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

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902 pLNCX myr HA Akt1 K179M

Plasmid

#9006

Depositor

William Sellers

Article

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

Akt1 K179M (AKT1 Human)

Use

Retroviral

Tags

HA and myr

Expression

Mammalian

Mutation

K179M. kinase dead.

Available Since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only

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pLJC5-3XHA-EGFP-PEX26

Plasmid

#139054

Purpose

HA-PEROXO Lentiviral Construct. Expresses a Peroxisomal Tag.

Depositor

David Sabatini

Article

Ray et al iScience. 2020 May 22;23(5):101109. doi:

Insert

3XHA-EGFP-PEX26

Use

Lentiviral

Tags

HA

Promoter

UBC

Available Since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR11 CA NFAT1

Plasmid

#11792

Depositor

Anjana Rao

Insert

NFAT1 (Nfatc2 Mouse)

Tags

HA

Expression

Bacterial

Mutation

Constitutively active: multiple serine to alanine…

Available Since

Aug. 7, 2006

Availability

Academic Institutions and Nonprofits only

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LAMP1-GFP

Plasmid

#16290

Depositor

Ron Vale

Article

Minin et al J Cell Sci. 2006 Feb 15. 119(Pt 4):659-7

Insert

LAMP1/RE72002

Tags

EGFP

Expression

Insect

Promoter

Ac5

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

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Myc-PSF-WT

Plasmid

#35183

Depositor

Benjamin Blencowe

Article

Rosonina et al Mol Cell Biol. 2005 Aug;25(15):6734-46.

Insert

PSF (SFPQ Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only

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pEGFP-hsp27 wt FL

Plasmid

#17444

Depositor

Andrea Doseff

Article

Voss et al J Biol Chem. 2007 Aug 24. 282(34):25088-

Insert

small heat shock protein27 (HSPB1 Human)

Tags

GFP

Expression

Mammalian

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only

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CLuc-FKBP

Plasmid

#31184

Depositor

David Piwnica-Worms

Article

Luker et al Proc Natl Acad Sci U S A. 2004 Aug 17. 1

Insert

C-terminus of Firefly Luciferase and FK506-binding protein 12 (FKBP1A Human)

Expression

Mammalian

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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pCasAb-apr

Plasmid

#121998

Purpose

Bacterial expression of Cas9 nuclease and RecAb recombination system in Acinetobacter baumannii

Depositor

Quanjiang Ji

Article

Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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