We narrowed to 11,069 results for: ena

1. pHR EGFR-SLP76-TagBFP

   Plasmid

   #188630

   Purpose

   Human EGFR labeled with SLP76 for monitoring with SLP76/VISH2 pYtag biosensor

   Depositor

   Jared Toettcher

   Article

   Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

   Insert

   EGFR (EGFR Human, Synthetic)
   

   Use

   Lentiviral

   Tags

   SLP76-TagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIRES-hrGFP II-mTET1_CD

   Plasmid

   #83571

   Purpose

   Expresses a mutant catalytic domain of TET1 in mammalian cells

   Depositor

   Jean-Pierre Issa

   Article

   Jin et al Nucleic Acids Res. 2014 Jun;42(11):6956-

   Insert

   Tet Methylcytosine Dioxygenase 1 (TET1 Human)
   

   Use

   Tags

   3X FLAG tag and hr GFP II

   Expression

   Mammalian

   Mutation

   TET1 amino acids 1-1417 deleted, catalytic domain…

   Promoter

   CMV

   Available since

   Sept. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDF1-GRM3 WT

   Plasmid

   #31798

   Depositor

   Yardena Samuels

   Article

   Prickett et al Nat Genet. 2011 Sep 25;43(11):1119-26. d

   Insert

   GRM3 (GRM3 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

4. pUC19 EGFRup-CD3ε-mNeonGreen-EGFRdown

   Plasmid

   #188634

   Purpose

   HDR template for CD3ε-mNeonGreen knock-in at C-terminus of human EGFR

   Depositor

   Jared Toettcher

   Article

   Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

   Insert

   EGFR (EGFR Human, Synthetic)
   

   Use

   CRISPR

   Tags

   CD3ε-mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. mARGAna (cassette 1, transient)

   Plasmid

   #197588

   Purpose

   Second-generation mammalian acoustic reporter gene derived from Anabaena

   Depositor

   Mikhail Shapiro

   Article

   Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   mARGAna (cassette 1)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGG-9.47_VP2_AS #2562

   Plasmid

   #74238

   Purpose

   Modified plasmid with a different backbone that yields higher titers of AAV-AS vectors. Encodes VP2-AS chimeric protein with 19 alanines fused to N-terminus of AAV9.47 VP2.

   Depositor

   Miguel Sena-Esteves

   Article

   Choudhury et al Mol Ther. 2015 Dec 28. doi: 10.1038/mt.2

   Inserts

   AAV2 Rep
   AAV9.47 VP2-AS
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNK3203

   Plasmid

   #219749

   Purpose

   DVK_AF CIDAR vector encoding hispidin-synthase from Mycena citricolor under control of CMV promoter, for mammalian expression

   Depositor

   Alexander Mishin

   Article

   Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

   Insert

   pCMV - mcitHispS - tSV40
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIRES-hrGFP II-mTET1

   Plasmid

   #83569

   Purpose

   Expresses TET1 with a mutated catalytic domain in mammalian cells

   Depositor

   Jean-Pierre Issa

   Article

   Jin et al Nucleic Acids Res. 2014 Jun;42(11):6956-

   Insert

   Tet Methylcytosine Dioxygenase 1 (TET1 Human)
   

   Use

   Tags

   3X FLAG tag and hr GFP II

   Expression

   Mammalian

   Mutation

   catalytic domain mutant H1672D, D1674A

   Promoter

   CMV

   Available since

   Sept. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. MiniCoopR 2xU6:gRNA pten, mitfa:Cas9

   Plasmid

   #118845

   Purpose

   Targets ptena and ptenb and expresses zebrafish mitfa specifically in zebrafish melanocytes

   Depositor

   Leonard Zon

   Article

   Ablain et al Science. 2018 Nov 1. pii: science.aau650

   Insert

   ptena and ptenb (ptena, ptenb Zebrafish)
   

   Use

   CRISPR; Tol2

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFB-U6-Chrna5-gRNA-hSyn-mCherry-WPRE-SV40pA

    Plasmid

    #128340

    Purpose

    pAAV encoding gRNA sequence for loss-of-function indels

    Depositor

    Ryan Drenan

    Article

    Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

    Insert

    Chrna5 (Chrna5 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV Syn hBE Rh Guanylyl Cyclase1 2A tDimer

    Plasmid

    #66779

    Purpose

    The rhodopsin-guanylyl cyclase of the aquatic fungus Blastocladiella emersonii enables fast optical control of cGMP signaling

    Depositor

    Thomas Oertner

    Article

    Scheib et al Sci Signal. 2015 Aug 11;8(389):rs8. doi:

    Inserts

    hbeRhGC
    red fluorescent protein
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human Synapsin

    Available since

    Oct. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. MtPT4-p3

    Plasmid

    #160003

    Purpose

    Multigene construct containing the betalain pigment biosynthetic genes under the control of the MtPT4 promoter for visualisation of arbuscular mycorrhizal colonisation in Medicago truncatula roots.

    Depositor

    Sam Brockington

    Article

    Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

    Inserts

    DsRed (reverse orientation)
    DODAα1
    CYP76AD1
    cDOPA5GT
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Arabidopsis thaliana Ubiquitin 10 Promoter (AtUb1…

    Available since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-S586A-VLCAD-FLAG

    Plasmid

    #55824

    Purpose

    Expresses S586A VLCAD mutant in mammalian cells

    Depositor

    Yoshimi Homma

    Article

    Kabuyama et al Am J Physiol Cell Physiol. 2010 Jan;298(

    Insert

    Very long-chain specific acyl-CoA dehydrogenase (ACADVL Human)
    

    Use

    Tags

    FLAG tag

    Expression

    Mammalian

    Mutation

    changed Serine 586 toAlanine

    Promoter

    CMV

    Available since

    July 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAR-9.47_VP2_AS

    Plasmid

    #74241

    Purpose

    Original plasmid described in Choudhury et al, 2015 Mol. Ther. for production of AAV-AS vectors. Encodes VP2_AS chimeric protein with 19 alanines fused to the N-terminus of AAV9.47 VP2.

    Depositor

    Miguel Sena-Esteves

    Article

    Choudhury et al Mol Ther. 2015 Dec 28. doi: 10.1038/mt.2

    Inserts

    AAV2 Rep
    AAV9.47-VP2_AS
    

    Use

    AAV

    Tags

    19 alanines fused to VP2 of AAV9.47

    Expression

    Mutation

    Promoter

    Available since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-nAChr-Alpha6

    Plasmid

    #48812

    Purpose

    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

    Depositor

    Henry Lester

    Article

    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

    Insert

    nAChr-alpha6 (Chrna6 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET21b-LOV-ipaA L514K L531E

    Plasmid

    #40253

    Depositor

    Brian Kuhlman

    Article

    Lungu et al Chem Biol. 2012 Apr 20;19(4):507-17.

    Insert

    LOV-ipaA L514K L531E
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    L514K; L531E; Jα helix region hybridized (AsLOV2 …

    Promoter

    T7

    Available since

    Sept. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA4/TO/GFPZNF598-9P-9A

    Plasmid

    #141192

    Purpose

    Expresses GFP-ZNF598 mutated in polyproline streches in mammalian cells, can be used to make inducible cell line

    Depositor

    Simon Bekker-Jensen

    Article

    Tollenaere et al Cell Rep. 2019 Mar 26;26(13):3511-3521.e

    Insert

    ZNF598 (ZNF598 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    all 3 proline repeats are mutated to Alanine (9xP…

    Promoter

    CMV

    Available since

    June 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA3.1-nAChr-beta3

    Plasmid

    #48811

    Purpose

    This report is the first to directly measure nAChR subunit stoichiometry using FRET and plasma membrane localization of α6- and β3-containing receptors using TIRF.

    Depositor

    Henry Lester, Jerry Stitzel

    Article

    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

    Insert

    nAChr Beta 3 subunit, isoform 1 (Chrnb3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    *

    Promoter

    CMV

    Available since

    Sept. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. C-His-GvpC-Calpain

    Plasmid

    #153295

    Purpose

    Expresses calpain-sensitive variant of Anabaena flos-aquae GvpC in E.coli

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

    Insert

    Calpain-sensitive Gas vesicle protein C (his-tagged)
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    1) Contains an extra glycine after the methionine…

    Promoter

    T7

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. delNC C-His Ana GvpC in pET28a

    Plasmid

    #85736

    Purpose

    Expresses a truncated His-tagged GvpC from Anabaena flos-aquae without the N-and C-terminal regions in E.coli

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al ACS Nano. 2016 Aug 23;10(8):7314-22. doi

    Insert

    Gas vesicle protein C (truncated and his-tagged)
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Deletion of N and C terminal regions, addition of…

    Promoter

    T7

    Available since

    March 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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