We narrowed to 4,296 results for: p2a

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pMK433 (mAID-P2A-BSD)

Plasmid

#168228

Purpose

C-terminal tagging with mAID

Depositor

Masato Kanemaki

Article

Saito et al Curr Protoc. 2021 Aug;1(8):e219. doi: 10

Insert

mAID-P2A-BSD

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pVH64-Tier1-PSV40-Citrine_p2a_SEAP

Plasmid

#169558

Purpose

Tier-1 vector encoding PSV40-driven Citrine-p2A-SEAP expression (PSV40-Citrine-p2A-SEAP-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PSV40-driven citrine-P2A-SEAP fusion construct

Expression

Mammalian

Promoter

PSV40

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-xCas9RA-P2A-Puro

Plasmid

#110871

Purpose

Lentiviral vector for constitutive expression of xCas9(3.7)RA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

xCas9(3.7)RA

Use

Lentiviral

Tags

FLAG

Mutation

A262T, R324L, S409I, E480K, E543D, M694I, E1219V

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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EGFP-P2A-APEX2-csChBP

Plasmid

#108877

Purpose

Expression of APEX2 fused to a conditionally stable nanobody against mCherry (includes green cytoplasmic reporter)

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

EGFP-P2A-APEX2-csChBP

Expression

Mammalian

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA4TO-24xGCN4_v1-P2A-sfGFP

Plasmid

#61057

Purpose

sfGFP and the 24xGCN4_v1 peptide array are expressed from the same transcript, separated by a P2A site

Depositor

Ron Vale

Article

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

24xGCN4_v1 and sfGFP

Expression

Mammalian

Available Since

Nov. 17, 2014

Availability

Academic Institutions and Nonprofits only
BE3-P2A-EGFP (pJUL977)

Plasmid

#123612

Purpose

CAG promoter expression plasmid for rAPOBEC1-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP.

Depositor

Keith Joung

Article

Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

Insert

BE3-P2A-EGFP

Expression

Mammalian

Mutation

D10A in SpCas9

Promoter

CAG

Available Since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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PuroR-P2A-DD-TCOF1-donor

Plasmid

#60747

Purpose

Donor plasmid for HR-mediated recombination to insert puromycin resistance and append a destabilization domain to the N-terminus of TCOF1

Depositor

Benhur Lee

Article

Park et al PLoS One. 2014 Apr 17;9(4):e95101. doi:

Insert

TCOF1 (TCOF1 Human)

Use

Targeting donor

Available Since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only
pCMVlac-dirTopo-P2A-aGFP

Plasmid

#61972

Purpose

A directional cloning vector of PCR-amplified cDNAs for mammalian expression

Depositor

Hiroshi Udo

Inserts

lacZ alpha
EGFP

Expression

Mammalian

Mutation

It contains silent mutations

Available Since

Feb. 4, 2015

Availability

Academic Institutions and Nonprofits only

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BlastR-P2A-DD-TCOF1-donor

Plasmid

#60748

Purpose

Donor plasmid for HR-mediated recombination to insert blasticidin resistance and append a destabilization domain to the N-terminus of TCOF1

Depositor

Benhur Lee

Article

Park et al PLoS One. 2014 Apr 17;9(4):e95101. doi:

Insert

TCOF1 (TCOF1 Human)

Use

Targeting donor

Available Since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only
pLV-H1-shSRGAP2A-hsynapsin-EGFP

Plasmid

#220798

Purpose

shRNA plasmid against human SRGAP2A gene with EGFP expression in neurons

Depositor

Pierre Vanderhaeghen

Article

Libe-Philippot et al Neuron. 2024 Oct 9:S0896-6273(24)00645-7

Insert

EGFP

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pNOC-CRISPR-P2A-BlastR

Plasmid

#98147

Purpose

Expresses Cas9-Nlux-P2A-BlastR and sgRNA in Nannochloropsis oceanica.

Depositor

Eva Farre

Article

Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

Type

Empty backbone

Use

Algae, nannochloropsis expression

Tags

P2A-BlastR fusion with Cas9-Nlux

Promoter

Ribi promoter

Available Since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pMK377 (Hygro-P2A-mCherry2)

Plasmid

#121188

Purpose

N-terminal tagging

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

Hygro-P2A-mCherry2

Use

CRISPR

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pMKO.1-Puro-shPP2A-Aalpha

Plasmid

#15249

Depositor

William Hahn

Article

Sablina et al Cell. 2007 Jun 1. 129(5):969-82.

Insert

PP2A regulatory subunit A, alpha isoform (PPP2R1A Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

July 12, 2007

Availability

Academic Institutions and Nonprofits only
CaMKIIa-mScarlet-I-P2A-2M-Qt-FLAG

Plasmid

#206956

Purpose

AAV plasmid for CaMKIIa driven co-expression of mScarlet-I and 2M-Qt-FLAG EMcapsulins

Depositor

Gil Westmeyer

Article

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

mScarlet-I, MmMT3, QtEnc

Use

AAV

Tags

FLAG

Available Since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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ICP8-P2A/Crimson (pSLIK4)

Plasmid

#113859

Purpose

For Doxycycline-inducible expression of ICP8 synaptase gene, with ICP8-P2A-red fluorescent protein gene E2-Crimson

Depositor

Richard S Myers

Article

Valledor et al PLoS One. 2018 Aug 15;13(8):e0200955. do

Insert

ICP8

Use

Lentiviral

Tags

P2A/Crimson

Available Since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pUC-dirTopo-P2A-aGFP

Plasmid

#61974

Purpose

A directional cloning vector of PCR-amplified cDNAs for mammalian expression

Depositor

Hiroshi Udo

Inserts

lacZ alpha
EGFP

Expression

Mammalian

Mutation

It contains silent mutations

Available Since

Feb. 4, 2015

Availability

Academic Institutions and Nonprofits only

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JDW 947 (pME-mmTcf4_DN_P2A_mCherry)

Plasmid

#232122

Purpose

A middle entry gateway clone containing myc-tagged murine dominant negative Tcf4 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

Tcf4ΔN (Tcf4 Mouse)

Use

Gateway cloning

Tags

myc

Mutation

lacks aa 1-56

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-postASAP-p2a-ChrimsonR

Plasmid

#178794

Purpose

Expresses voltage sensor targeted to dendrites and spines along with ChrimsonR

Depositor

Rafael Yuste

Article

Cornejo et al Science. 2022 Jan 7;375(6576):82-86. doi

Insert

FingR.PSD95, ASAP2f and ChrimsonR

Tags

mRuby2

Expression

Mammalian

Mutation

mutations in amino acids L146G, S147T N149R, S150…

Promoter

CAG

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pJL2-mGold-P2A-EBFP2

Plasmid

#157998

Purpose

Co-expression of mGold and EBFP2 in mammalian cells.

Depositor

Francois St-Pierre

Article

Lee et al Sci Adv. 2020 Oct 23;6(43). pii: 6/43/ea

Insert

mGold-P2A-EBFP2

Expression

Mammalian

Mutation

mGold is mVenus with L46F;T63S mutations

Promoter

CMV

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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katushka-FMDV-P2A-pTRIP

Plasmid

#163522

Purpose

Used to construct lentivirus to express katushka in mammalian cells with option for a second insert

Depositor

Thomas Weber

Insert

stopless Katushka-P2A

Use

Lentiviral

Expression

Bacterial and Mammalian

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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