We narrowed to 4,062 results for: p2a

1. pMK376 (Hygro-P2A-mClover)

   Plasmid

   #121187

   Purpose

   N-terminal tagging

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

   Insert

   Hygro-P2A-mClover
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKLV2-U6gRNA5(Empty)-PGKGFP2ABFP-W

   Plasmid

   #67983

   Purpose

   Cas9 activity reporter (control) with GFP and BFP

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   U6gRNA cassette, PGKGFP2ABFP cassette, WPRE
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Deleted BbsI site within WPRE, modified BFP

   Promoter

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-xBE3-P2A-Puro

   Plasmid

   #110870

   Purpose

   Lentiviral vector for constitutive expression of xCas9(3.7)-BE3 in mammalian cells

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   xCas9(3.7)-BE3
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5-FRT/T0-Flag-CIP2A

   Plasmid

   #222623

   Purpose

   mammalian expression vector of Flag-tagged CIP2A

   Depositor

   Daniel Durocher

   Article

   Adam et al Nat Cancer. 2021 Dec;2(12):1357-1371. do

   Insert

   CIP2A (CIP2A Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 18, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pFH2.16_MiniGagZip-MCP_Zip-P2A-eGFP

   Plasmid

   #205531

   Purpose

   Express HIV MiniGagZip-MCP_Zip-P2A-eGFP

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   MiniGagZip-MCP_Zip
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMY-MCS-P2A-Ly6G

   Plasmid

   #163353

   Purpose

   Retroviral backbone for in-frame cloning of a gene of interest and a P2A separated Ly6G surface marker

   Depositor

   Louis Ates, Teunis Geijtenbeek

   Article

   van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMY-eGFP-P2A-PuroR-HA

   Plasmid

   #163343

   Purpose

   Retroviral vector to express cytosolic GFP and an HA-tagged puromycin selection marker

   Depositor

   Louis Ates, Teunis Geijtenbeek

   Article

   van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

   Insert

   GFP
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMY-CD3-P2A-CD90.2

   Plasmid

   #163338

   Purpose

   Retroviral vector to overexpress the murine CD3 (TCR) components CD3 gamma, CD delta, CD epsilon and TCR zeta and surface marker CD90.2, separated by T2; F2A; E2A; P2A respectively, under control of the LTR promoter

   Depositor

   Louis Ates, Teunis Geijtenbeek

   Article

   van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

   Insert

   CD3
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMKO.1-Puro-shPP2A-Abeta

   Plasmid

   #15250

   Depositor

   William Hahn

   Article

   Sablina et al Cell. 2007 Jun 1. 129(5):969-82.

   Insert

   PP2A regulatory subunit A, beta isoform (PPP2R1B Human)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 12, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pME-mKate-P2A-APEX2-NS

    Plasmid

    #67670

    Purpose

    Gateway entry clone containing mKate2-P2A-APEX2 with no stop codon

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    mKate2-P2A-APEX2-NS
    

    Use

    Gateway multisite middle entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMY-mCherry-P2A-PuroR-HA

    Plasmid

    #163342

    Purpose

    Retroviral vector to express cytosolic mCherry and an HA-tagged puromycin selection marker

    Depositor

    Louis Ates, Teunis Geijtenbeek

    Article

    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

    Insert

    mCherry
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-BE3RA-P2A-Puro

    Plasmid

    #110839

    Purpose

    Lentiviral vector for constitutive expression of BE3RA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3RA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    D10A

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSV40-GUS-P2A-Hygro

    Plasmid

    #106480

    Purpose

    Expression of GUS and Hygromycin resistance gene from SV40 promoter

    Depositor

    Alex Hewitt

    Inserts

    beta-glucuronidase
    Hygromycin B resistance
    

    Use

    Tags

    Expression

    Mammalian and Plant

    Mutation

    Promoter

    SV40 promoter

    Available since

    April 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. AKT-APEX2-P2A-mKate2

    Plasmid

    #67654

    Purpose

    Mammalian expression vector driving AKT-APEX2 (Human AKT-PH domain, [marks PI(3,4)P2 and PI(3,4,5)P3] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    AKT-PH-APEX2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. BTK-APEX2-P2A-mKate2

    Plasmid

    #67653

    Purpose

    Mammalian expression vector driving BTK-APEX2 (Human BTK-PH domain, [marks PI(3,4,5)P3] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red fluorescent reporter.

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    BTK-PH-APEX2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. Thy1.2-NLS_HA_Dre-P2A-BFP

    Plasmid

    #51275

    Purpose

    Co-expression of the site-specific recombinase Dre and BFP under the control of the Thy1.2 expression cassette

    Depositor

    Pawel Pelczar

    Article

    Hermann et al Nucleic Acids Res. 2014 Jan 9.

    Insert

    NLS_HA_Dre-P2A-BFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Thy1.2

    Available since

    March 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti/TO/EYFP-P2A-IDH1(HA)

    Plasmid

    #122482

    Purpose

    A lentiviral vector (pLenti6.3/TO/DEST) expresses EYFP, P2A, and IDH1 C-terminally tagged with HA

    Depositor

    Eric Huang

    Article

    Tiburcio et al Acta Neuropathol. 2018 Feb;135(2):285-29

    Insert

    isocitrate dehydrogenase 1 (IDH1 Human)
    

    Use

    Lentiviral

    Tags

    HA and P2A

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TO

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKLV-EF1aGFP2AKAT7-linker-mAID-W

    Plasmid

    #159294

    Purpose

    Lentiviral vector expressing GFP and KAT7 (gRNA-resistant, mAID-tagged) driven by human EF1a promoter

    Depositor

    Kosuke Yusa

    Article

    Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

    Insert

    KAT7 (KAT7 Human)
    

    Use

    Lentiviral

    Tags

    mAID (auxin-inducible degron)-tagged

    Expression

    Mammalian

    Mutation

    CRISPR sites mutated (gRNA ID. 5 and A10)

    Promoter

    human EF1a promoter

    Available since

    Oct. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-FNLSHiFi-P2A-Puro

    Plasmid

    #136902

    Purpose

    Lentivirus for constitutive expression of FNLSHiFi in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    FNLSHiFi
    

    Use

    Lentiviral

    Tags

    FLAG-NLS and NLS

    Expression

    Mutation

    R691A

    Promoter

    EF1s

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-HF1RA-P2A-Puro

    Plasmid

    #110853

    Purpose

    Lentiviral vector for constitutive expression of Cas9-HF1RA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-HF1RA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    R661A, Q695A, Q926A, and NLS sequence at the N-te…

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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