19,660 results

1. lenti-SpCas9 puro

   Plasmid

   #104994

   Purpose

   This 3rd generation lentiviral construct delivers hSpCas9 and puromycin resistance.

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Insert

   KpnI-XhoI-BsrGI-NheI
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pNFkB:eGFP

   Plasmid

   #44922

   Depositor

   John Rawls

   Article

   Kanther et al Gastroenterology. 2011 Jul;141(1):197-20

   Insert

   EGFP
   

   Available Since

   Sept. 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLVX-M-Flag-BAP1-C91A

   Plasmid

   #125841

   Purpose

   express mutant BAP1-C91A

   Depositor

   Boyi Gan

   Article

   Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

   Insert

   BAP1 (BRCA1 associated protein 1) (BAP1 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Mutation

   C91A

   Available Since

   June 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pdestMB14_sur5p-dCas9-VP64

   Plasmid

   #177788

   Purpose

   Overexpression of dCas9-VP64 in C. elegans

   Depositor

   Michael Ristow

   Article

   Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

   Insert

   dCas9-VP64
   

   Expression

   Worm

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBbA8k-RFP

   Plasmid

   #35273

   Depositor

   Jay Keasling

   Article

   Lee et al J Biol Eng. 2011 Sep 20;5:12.

   Insert

   RFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   BAD

   Available Since

   Feb. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. mCherry-Mem

   Plasmid

   #55779

   Purpose

   This red fluorescent plasma membrane marker consists of the first twenty residues of neuromodulin fused to mCherry.

   Depositor

   Catherine Berlot

   Article

   Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep

   Insert

   mCherry-Mem
   

   Tags

   The N-terminal 20 residues of neuromodulin are fu…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGEX6P1-3xMBT

   Plasmid

   #46987

   Purpose

   Inducible expression of 3xMBT from L3MBTL1 as a GST fusion protein in E.coli

   Depositor

   Or Gozani

   Article

   Moore et al Mol Cell. 2013 May 9;50(3):444-56. doi:

   Insert

   Triple MBT domain from human L3MBTL1 (L3MBTL1 Human)
   

   Tags

   GST and PreScission cleavage site

   Expression

   Bacterial

   Mutation

   contains amino acids 190–530 of accession NP_0562…

   Promoter

   tac

   Available Since

   Sept. 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFA6a-13Myc-kanMX6

   Plasmid

   #39294

   Depositor

   Jurg Bahler, John Pringle

   Article

   Bahler et al Yeast. 1998 Jul;14(10):943-51.

   Insert

   KanR
   

   Use

   Yeast genomic targeting

   Tags

   13Myc, A. gossypii translation elongation factor …

   Available Since

   Aug. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AttB_ACE2_IRES-mCherry-H2A-P2A-PuroR

   Plasmid

   #171594

   Purpose

   Strong human ACE2 abundance from Bxb1 landing pad

   Depositor

   Kenneth Matreyek

   Article

   Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

   Inserts

   ACE2 (ACE2 Human)
   mCherry
   H2A
   Pac
   

   Use

   Synthetic Biology; Promoterless bxb1 dna recombin…

   Expression

   Mammalian

   Available Since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-TLR10-YFP

    Plasmid

    #13643

    Depositor

    Doug Golenbock

    Insert

    Toll-Like Receptor 10 (TLR10 Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Available Since

    June 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDestTol2pA2-9xGRE

    Plasmid

    #74736

    Purpose

    GRE-dependent EGFP reporter construct flanked by the minimal Tol2 transposon elements

    Depositor

    Luisa Dalla Valle

    Article

    Benato et al Mol Cell Endocrinol. 2014 Jul 5;392(1-2)

    Insert

    EGFP
    

    Use

    Zebrafish expression

    Promoter

    nine GC-responsive elements (9xGRE) from the rat …

    Available Since

    April 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRK GFP Paxillin

    Plasmid

    #50529

    Purpose

    This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

    Depositor

    Kenneth Yamada

    Insert

    Paxillin (PXN Human)
    

    Tags

    GFP21

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBS-Hsp70-Cas9

    Plasmid

    #46294

    Purpose

    A codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter.

    Depositor

    Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger

    Article

    Gratz et al Genetics. 2014 Apr;196(4):961-71. doi: 1

    Insert

    codon optimized Cas9
    

    Use

    CRISPR

    Tags

    3x Flag

    Expression

    Insect

    Mutation

    Silent change from C to A at bp 834 of insert

    Promoter

    Hsp70

    Available Since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Flag-HA-USP2

    Plasmid

    #22577

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    USP2 (USP2 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Nov. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. nAChR alpha4 WT

    Plasmid

    #24271

    Depositor

    Henry Lester

    Article

    Nashmi et al J Neurosci. 2003 Dec 17. 23(37):11554-67

    Insert

    nAChR alpha4 WT (Chrna4 Mouse)
    

    Expression

    Mammalian

    Available Since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

16. AAVS1-Puro CAG FUCCI

    Plasmid

    #136934

    Purpose

    donor plasmid for constitutive FUCCI expression in human cells

    Depositor

    Xiaoping Bao

    Article

    Chang et al Biotechnol Bioeng. 2020 Apr 11. doi: 10.

    Insert

    Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120)
    

    Use

    Synthetic Biology

    Tags

    Clover and mKO2

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pECE-M2-BAIAP2 wt

    Plasmid

    #31656

    Depositor

    Anne Brunet

    Article

    Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

    Insert

    BAI1-associated protein 2 (BAIAP2 Human)
    

    Tags

    M2

    Expression

    Mammalian

    Available Since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pEEV2/11

    Plasmid

    #166888

    Purpose

    Helper/packaging plasmid for production of AAV2/11 pseudotype vector

    Depositor

    Tadahito Kanda, Seiichiro Mori

    Article

    Mori et al Virology. 2004 Dec 20;330(2):375-83. doi

    Insert

    AAV2 rep/AAV11 cap
    

    Use

    AAV

    Promoter

    AAV2 p5

    Available Since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDEST-eGFP-C1-VPS35

    Plasmid

    #163622

    Purpose

    human VPS35 cDNA C-terminally tagged with eGFP

    Depositor

    Josef Kittler

    Article

    Halff et al Cell Rep. 2019 Nov 26;29(9):2599-2607.e6

    Insert

    human VPS35 (VPS35 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLVX-EF1alpha-SARS-CoV-2-N-2xStrep-IRES-Puro

    Plasmid

    #141391

    Purpose

    Lentiviral expression of SARS-CoV-2 nucleocapsid (N) protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    SARS-CoV-2-N (N SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available Since

    April 8, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge