We narrowed to 8,643 results for: SARS

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tet-pLKO-sgRNA-puro

Plasmid

#104321

Purpose

3rd generation lentiviral plasmid for inducible expression of sgRNA; derived from tet-pLKO-puro; puromycin selection. See manual for detailed protocols.

Depositor

Nathanael Gray

Article

Huang et al eLife. 2017 Sep 19;6. pii: e26693. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

H1/TO

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pGST2-GST-TEV-NDP52

Plasmid

#187828

Purpose

Plasmid for the expression and purification of GST-TEV-NDP52 in E. coli Rosetta pLysS. Internal reference: SMC1586

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

NDP52 (CALCOCO2 Human)

Tags

GST-TEV

Expression

Bacterial

Available Since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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EF.STAT3DN.Ubc.GFP

Plasmid

#24984

Purpose

Depositor

Linzhao Cheng

Article

Hillion et al Cancer Research 68, 10121, December 2008

Insert

STAT3 (STAT3 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Y705F--dominant negative

Available Since

June 14, 2010

Availability

Academic Institutions and Nonprofits only

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pOG44_FLP_recombinase (pEHA1338)

Plasmid

#209087

Purpose

FLP recombinase for FRT-mediated integration

Depositor

Vikram Khurana

Article

Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

Insert

FLP recombinase

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-CDH5-mCitrine-IRES-puro

Plasmid

#85143

Purpose

lentiviral expression of human VE-cadherin, C-terminally tagged with mCitrine

Depositor

Tobias Meyer

Article

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Insert

CDH5-mCitrine (VE-cadherin, C-terminally tagged with mCitrine (CDH5 Human)

Use

Lentiviral

Tags

mCitrine

Promoter

CAG

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pWal10-roe-sfGFP

Plasmid

#240239

Purpose

Gateway destination plasmid to generate C-terminal sfGFP fusions under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv [Preprint]. 2025 Jul 11:2025.07.

Type

Empty backbone

Use

Gateway destination vector

Expression

Insect

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-mTurquoise-MLC-IRES-neo

Plasmid

#85145

Purpose

lentiviral expression of myosin light chain (MLC, MYL9), N-terminally tagged with mTurquoise

Depositor

Tobias Meyer

Article

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Insert

mTurquoise-MLC (myosin regulatory light chain, N-terminally tagged with mTurquoise (MYL9 Human)

Use

Lentiviral

Tags

mTurquoise

Promoter

EF1a

Available Since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

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Strep-KIFC1*-mCh

Plasmid

#120169

Purpose

Expresses a chimera of streptavidin, a KIFC1-based retrograde motor and mCherry (fluorescent tag)

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

kinesin family member C1 (KIFC1 Human)

Tags

Streptavidin and mCherry

Expression

Mammalian

Mutation

Truncated KIFC1: 125-673 aa

Promoter

CMV

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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mCh-KIF5B*-strep

Plasmid

#120164

Purpose

Expresses a chimera of mCherry (fluorescent tag), a KIF5B-based anterograde motor and streptavidin

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

kinesin family member 5B (Kif5b Mouse)

Tags

Streptavidin and mCherry

Expression

Mammalian

Mutation

Truncated KIF5B: 1-555 aa

Promoter

CMV

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pMXS-GshF-blasticidin

Plasmid

#210313

Purpose

Expresses humanized Streptococcus thermophilus GshF in mammalian cells

Depositor

Kivanc Birsoy

Article

Wang et al Nature. 2021 Nov;599(7883):136-140. doi:

Insert

Bifunctional glutamate-cysteine ligase/glutathione synthetase GshF (gshAB Streptococcus thermophilus)

Use

Retroviral

Tags

FLAG peptide

Mutation

Codon optimized for expression in human cells

Available Since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pTXB1-EXO1b D173A

Plasmid

#68269

Purpose

Bacterial expression of human EXO1 D173A (catalytically-dead) mutant

Depositor

Stefano Ferrari

Article

Eid et al EMBO Rep. 2010 Dec;11(12):962-8. doi: 10

Insert

EXO1 (EXO1 Human)

Tags

Mxe intein/chitin binding domain

Expression

Bacterial

Mutation

catalytically-dead D173A. Please see depositor co…

Promoter

T7

Available Since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only
mCh-KIF1A*-strep

Plasmid

#120165

Purpose

Expresses a chimera of mCherry (fluorescent tag), a KIF1A-based anterograde motor and streptavidin

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

kinesin family member 1A (Kif1a Mouse)

Tags

Streptavidin and mCherry

Expression

Mammalian

Mutation

Truncated KIF1A: 1-405 aa

Promoter

CMV

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pCEFL_GPR161-HA

Plasmid

#249268

Purpose

Expression of GPR161 wild type

Depositor

Ramiro Iglesias-Bartolome

Article

Krantz et al Cancer Res. 2025 Dec 29. doi: 10.1158/00

Insert

GPR161 (GPR161 Human)

Tags

HA

Expression

Mammalian

Promoter

EF-1

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pCIneoMyc UNC119Aa

Plasmid

#128331

Purpose

Mammalian expression vector of Myc-tagged human UNC119A isoform a

Depositor

Yutaka Hata

Article

Iwasa et al Cancer Sci. 2018 Sep;109(9):2767-2780. d

Insert

UNC119 (UNC119 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.2-FUS-1-526aa-V5

Plasmid

#29609

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS (FUS Human)

Tags

V5

Expression

Mammalian

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Linear Tau 0N4R 3X Flag tag

Plasmid

#194171

Purpose

Insert contains linear tau 0N4R with a 3X Flag tag. Expresses linear protein tau 0N4R with a 3X Flag tag for Immunoprecipitation.

Depositor

Stefan Stamm

Article

Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129

Insert

Microtubule-Associated Protein Tau 0N4R

Tags

3X Flag

Expression

Mammalian

Available Since

Jan. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pAS_4xU6-PylT M15 (UUA) FLAG-Mma PylRS

Plasmid

#154774

Purpose

plasmid with 4xMma PylT (M15 mutant, UUA anticodon) cassette and Mma PylRS for ochre suppression in transient or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

Mma PylRS (pylS Methanosarcina mazei)

Tags

FLAG

Expression

Mammalian

Mutation

in PylT: UUA anticodon and G10A, G14U, G16U, C20U…

Promoter

EF1

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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MCP-MSN

Plasmid

#210704

Purpose

This plasmid express EF1a promoter driven MCP-MSN transactivation module

Depositor

Isaac Hilton

Article

Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

Insert

MCP fused to transactivation domain from Human transcription factor MRTF-A, STAT-1 and NRF2

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

N55K in MCP

Promoter

EF1a

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pGP-20XUAS-IVS-Syn21-jGCaMP8s-p10

Plasmid

#162386

Purpose

Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Insert

jGCaMP8s

Tags

6xHis

Expression

Insect

Mutation

S26M F286Y Q315H

Promoter

hsp70

Available Since

May 3, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCV-LTR-dCas9-VP64-BFP

Plasmid

#46912

Purpose

Human expression vector containing MSCV LTR promoter, dCas9 that is fused to 2x NLS, VP64 and tagBFP

Depositor

Stanley Qi, Jonathan Weissman

Article

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Inserts

dCas9-VP64-BFP fusion
Puromycin resistance

Use

CRISPR and Retroviral

Tags

3xNLS, BFP, and VP64 domain

Expression

Mammalian

Promoter

LTR and PGK

Available Since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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