We narrowed to 11,713 results for: NSI

1. pLX304_zeo_eGFP_V5

   Plasmid

   #160096

   Purpose

   Expresses eGFP in mammalian cells

   Depositor

   Rizwan Haq

   Article

   Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

   Insert

   eGFP
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pVL47 - pSCR>>

   Plasmid

   #116013

   Purpose

   destination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression

   Depositor

   Thomas Greb

   Article

   Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

   Insert

   pSCR:GR-LhG4:tSCR:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
   

   Tags

   HDEL and SP(ER)

   Expression

   Plant

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. Sumo3 PolQM1 deltains1

   Plasmid

   #78461

   Purpose

   Inducible expression in E. coli

   Depositor

   Sylvie Doublie, Susan Wallace

   Article

   Hogg et al J Mol Biol. 2011 Jan 21;405(3):642-52. d

   Insert

   Polymerase theta (POLQ Human)
   

   Tags

   His and SUMO3

   Expression

   Bacterial

   Mutation

   delta 1-1791; delta 2149-2170; Q2513R (please see…

   Promoter

   T7

   Available Since

   July 28, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pX330A-Yap1-E1-#1

   Plasmid

   #171517

   Purpose

   deletion of a genomic locus in Yap1 gene

   Depositor

   Hans Schöler

   Article

   Kagiwada et al Development. 2021 Oct 15;148(20). pii: 2

   Insert

   Yap1 (Yap1 Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330A-Yap1-E2-#1

   Plasmid

   #171518

   Purpose

   deletion of a genomic locus in Yap1 gene

   Depositor

   Hans Schöler

   Article

   Kagiwada et al Development. 2021 Oct 15;148(20). pii: 2

   Insert

   Yap1 (Yap1 Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5-FRT/TO-neo-C11orf51-IRES2-mRuby (2763)

   Plasmid

   #39858

   Depositor

   Jonathon Pines

   Article

   Mansfeld et al Nat Cell Biol. 2011 Sep 18;13(10):1234-4

   Insert

   C11orf51 (ANAPC15 Human)
   

   Tags

   IRES2-mRuby

   Expression

   Mammalian

   Available Since

   Oct. 31, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pAS_4xMx PylT C41CA_FLAG_Mx PylRS YA

   Plasmid

   #140014

   Purpose

   Plasmid with 4xMx PylT C41CA cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

   Insert

   Mx1201 PylRS YA (MMALV_RS05520 Methanomethylophilus alvus Mx1201)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Y126A, Y197F

   Promoter

   EF1

   Available Since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. Emerald-MannII-N-10

   Plasmid

   #54150

   Depositor

   Michael Davidson

   Article

   Planchon et al Nat Methods. 2011 May;8(5):417-23. doi:

   Insert

   MannII (Man2a1 Mouse)
   

   Tags

   Emerald

   Expression

   Mammalian

   Mutation

   aa 1-112 of MannII aa 1-112 of MannII

   Promoter

   CMV

   Available Since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

9. p260 pCMV-CRE-M-AR(wt)

   Plasmid

   #12494

   Depositor

   Jeffrey Green

   Article

   Kaczmarczyk et al Nucleic Acids Res. 2003 Aug 1. 31(15):e8

   Insert

   CRE-M AR (AR Human)
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Mutation

   CRE-M and AR ligand binding domain

   Available Since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

10. mEos3.2trLAT-2P

    Plasmid

    #203748

    Purpose

    Encodes the transmembrane domain from mouse LAT with mEos3.2 fused on the N terminus. Palmitoylation sites at C27 and C30 mutated to A. Used as a fluorescent plasma membrane probe.

    Depositor

    Sarah Veatch

    Article

    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

    Insert

    trLAToC27AC30A (Lat Mouse)
    

    Tags

    ER translocation signal - mEos3.2

    Expression

    Mammalian

    Mutation

    C27A, C30A

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHH0103_ARHGAP4-1/1

    Plasmid

    #91518

    Purpose

    Protein expression and purification of human SH3 domain construct ARHGAP4-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    ARHGAP4-1/1 (ARHGAP4 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHH0103_ARHGAP9-1/1

    Plasmid

    #91442

    Purpose

    Protein expression and purification of human SH3 domain construct ARHGAP9-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    ARHGAP9-1/1 (ARHGAP9 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNCST-AausFP3

    Plasmid

    #129501

    Purpose

    Expresses AausFP3 constitutively in E. coli (most strains)

    Depositor

    Nathan Shaner

    Article

    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

    Insert

    AausFP3
    

    Expression

    Bacterial

    Promoter

    synthetic constitutive (stationary phase) promote…

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLVX-mCherry-SPASTIN, F124D

    Plasmid

    #180648

    Purpose

    Lentiviral expression vector for SPASTIN. Has F124D mutation. Used for generating cell lines. Has N-terminal mCherry tag. Dox-inducible. SPASTIN starts on M87. Internal ID: WISP20-44.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    SPASTIN
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Starts at M87, F124D mutation, has siRNA resistan…

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTRE Tight ArchT-GFP

    Plasmid

    #79627

    Purpose

    Tetracycline-dependent expression of ArchT-EGFP in mammalian cells

    Depositor

    Yasunori Hayashi

    Article

    Sakaguchi et al PLoS One. 2015 Jun 15;10(6):e0130163. do

    Insert

    ArchT
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    ArchT is a more sensitive version of Arch (archae…

    Promoter

    Tet responsive Ptight promoter

    Available Since

    July 1, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pET28a_6HT-TBP6.7(R49A)

    Plasmid

    #112726

    Purpose

    Arg49 of TBP6.7 and other TBPs is the only arginine that was lab-evolved from wild-type U1A to play a significant role in TAR RNA recognition. Mutation results in ~230-fold reduction in Kd.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(R49A)
    

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    R49A

    Promoter

    T7

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pVL46 - pWOX4>>

    Plasmid

    #116012

    Purpose

    destination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression

    Depositor

    Thomas Greb

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    pWOX4:GR-LhG4:tWOX4:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
    

    Tags

    HDEL and SP(ER)

    Expression

    Plant

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. TRS6E1b-luc(-732/-721) mutant 5

    Plasmid

    #45388

    Depositor

    Thomas Gelehrter

    Article

    Song et al J Biol Chem. 1998 Nov 6;273(45):29287-90

    Insert

    6 copies of hPAI-1 promoter -732/-721 mutated at -725/723
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    -725/-723 mutated from gtt to ccc

    Available Since

    June 20, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3-FLAG-MTOR-D2512H

    Plasmid

    #69017

    Purpose

    activating MTOR mutation

    Depositor

    David Sabatini

    Article

    Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

    Insert

    MTOR-D2512H (MTOR Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    change Aspartate 2512 to Histidine

    Available Since

    Dec. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

20. MSCV-TRE-dsRed-miR30_shBrd9_1116-PGK-Venus-IRES-NeoR

    Plasmid

    #75136

    Purpose

    Retroviral expression plasmid encoding inducible shBrd9_1116 (targeting murine Brd9)

    Depositor

    Christopher Vakoc

    Article

    Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

    Insert

    shBrd9_1116
    

    Use

    Retroviral

    Available Since

    May 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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