We narrowed to 30,735 results for: PLE
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Plasmid#134598PurposeLow Phosphate inducible SBS (bonryl-diphosphate) synthase with C-terminal mCherry)DepositorInsertSBS-mCherry
TagsmCherryExpressionBacterialPromoteryibDpAvailable SinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-T7-CBD-hGLY
Plasmid#134940PurposeT7 based expression of human glycine acyltransferase with an N-terminal chitin binding domainDepositorInserthuman glycine acyltransferase 2 (GLYATL2 )
Tagschitin binding domainExpressionBacterialPromotert7Available SinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTKhc-yibDp-GFP-β20cp6
Plasmid#127060PurposeLow phosphate inducible circular permutation of GFP, which is a substrate of the lon proteaseDepositorInsertGFPcp6-beta20
TagsHis tagExpressionBacterialPromoteryibDAvailable SinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM328
Plasmid#134243PurposeMammalian expression construct expressing sgRNA against PLK1DepositorInsertgRNA targeting PLK1 3'
UseCRISPRExpressionMammalianAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pst39-NST-clone14
Plasmid#133421PurposeS. cerevisiae Nip1 (with C-terminal TEV cleavage site and strep-II tag), Sui1 and Tif5B6 (with N-terminal 7xHis tag and TEV cleavage set) coding sequences with intervening ribosome binding sites and other cis-elements for expression as a polycistron, T7 theta 10 promoter (and corresponding terminator after polycistron).DepositorExpressionBacterialMutationORF for Nip1 encodes aa 4-156 (not complete ORF),…Available SinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
MTK2_025
Plasmid#123717PurposeEncodes crippled human pEF1alpha as a Type 2 part to be used in the MTK systemDepositorInsertpEF1ac
ExpressionMammalianMutationCrippled Kozac sequenceAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
LC8-like
Plasmid#133115PurposeHuman LC8-like protein, codon optimised for Sf9 expression.DepositorAvailable SinceOct. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1 mouse Tankyrase1 ARC2-3 (308-655)
Plasmid#132637Purposeexpresses mouse Tankyrase1 ARC2-3 (308-655) in E. coliDepositorInsertTankyrase1 (308-655)
ExpressionBacterialAvailable SinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMtnA UTR delta 21-55 eGFP SV40
Plasmid#132653PurposeExpresses an eGFP mRNA from the Drosophila Metallothionein A promoter that has 20 nt of the MtnA 5' UTRDepositorInserteGFP
ExpressionInsectPromoterMtnAAvailable SinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMtnA UTR delta 41-55 eGFP SV40
Plasmid#132652PurposeExpresses an eGFP mRNA from the Drosophila Metallothionein A promoter that has 40 nt of the MtnA 5' UTRDepositorInserteGFP
ExpressionInsectPromoterMtnAAvailable SinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
S1_AscI_V5-gateway_Bsu36I
Plasmid#128467Purposedestination vector with N-terminal V5 tagDepositorInsertdestination vector with N-terminal V5 tag
ExpressionBacterialAvailable SinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
S2_AscI_HA-gateway_Bsu36I
Plasmid#128468Purposedestination vector with N-terminal HA tagDepositorInsertdestination vector with N-terminal HA tag
ExpressionBacterialAvailable SinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
2ERT2-AAVS1 TALEN-L
Plasmid#120544PurposeDrug inducible TALEN targeting the human AAVS1 locus for genome editingDepositorInsertERT2, hAAVS1 1L TALEN
UseLentiviralExpressionMammalianPromoterEF1αAvailable SinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
YSBE420:pNEO_CAC1_Vn_HOG1 terminator
Plasmid#122701PurposePlasmid for tagging proteins in Cryptococcus neoformans; N-terminal fragment of Venus proteinDepositorInsertN-terminal fragment of Venus protein
UseTagging proteins in cryptococcus neoformansAvailable SinceMay 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
YSBE418:pNAT_ACA1_Vc_HOG1 terminator
Plasmid#122700PurposePlasmid for tagging proteins in Cryptococcus neoformans; C-terminal fragment of Venus proteinDepositorInsertC-terminal fragment of Venus protein
UseTagging proteins in cryptococcus neoformansAvailable SinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGAD-Atg17(∆77-91)
Plasmid#121516PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
ExpressionYeastMutationdeleted amino acids 77-91Available SinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3xFLAG LacZ
Plasmid#83093PurposeGateway entry vector for inducible expression of LacZ with N-terminal 3xFLAG tagDepositorInsertLacZ
UseEntry vector for gateway cloningTags3xFLAGPromoterTRE tight promoterAvailable SinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
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6xmyc_cort_UAS-P
Plasmid#112991PurposeP element vector for transposon insertion of Myc-tagged Drosophila cort (Cdc20) into Drosophila genomeDepositorAvailable SinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
gnu cDNA LD12084
Plasmid#112973PurposeCloning vector carrying the gnu cDNADepositorInsertgnu (gnu Fly)
ExpressionBacterialAvailable SinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
mTalin2_R7R8
Plasmid#111041PurposePlasmid expresses the talin2 rod domains R7 and R8DepositorAvailable SinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 N4A 3xFLAG
Plasmid#111975PurposeShuffle plasmid containing the HSH155 gene and surrounding regions. It bears a TRP marker. It has 3xFLAG at the C-terminus. Contains the N4A mutation.DepositorInsertHsh155 N4A 3x FLAG
Tags3xFLAGExpressionBacterial and YeastMutationT178A R181A R182A R186AAvailable SinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 K818A 3xFLAG
Plasmid#111982PurposeShuffle plasmid containing the HSH155 gene and surrounding regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has 3xFLAG at the C-terminus. Contains K818A mutation. LethalDepositorInsertHsh155 K818A 3x FLAG
Tags3xFLAGExpressionBacterial and YeastMutationK818AAvailable SinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 R775A 3xFLAG
Plasmid#111977PurposeShuffle plasmid containing the HSH155 gene and surrounding regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has 3xFLAG at the C-terminus. Contains R775A mutation. LethalDepositorInsertHsh155 R775A 3x FLAG
Tags3xFLAGExpressionBacterial and YeastMutationR775AAvailable SinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 R778A 3xFLAG
Plasmid#111979PurposeShuffle plasmid containing the HSH155 gene and surrounding regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has 3xFLAG at the C-terminus. Contains R778A mutation. LethalDepositorInsertHsh155 R778A 3x FLAG
Tags3xFLAGExpressionBacterial and YeastMutationR778AAvailable SinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
mTalin2_R7
Plasmid#111039PurposePlasmid expresses the talin2 rod domain R7 a 5 helix bundle.DepositorAvailable SinceJune 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15b+R2(L171C)
Plasmid#108239PurposeExpresses PdR-PCNA2(L171C) fusion protein in E. coliDepositorInsertPdR-PCNA2(L171C) fusion protein
TagsHis6 tagExpressionBacterialMutationPCNA2,L171CPromoterT7 promoterAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15T+1X
Plasmid#108188PurposePCNA1-PdX fusion proteinDepositorInsertPCNA1-PdX fusion protein
TagsHis6 tagExpressionBacterialMutationC73S/C85S (PdX)PromoterT7 promoterAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ121
Plasmid#99325PurposeExpress two TALE-VP64 activator linked by in-frame T2A (encoding Thosea asigna 'self-cleaving' 2A peptide) sequenceDepositorInsertTALE-VP64-T2A-TALE-VP64
UseTALENExpressionPlantAvailable SinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJFRC81-3xVAS4-IVS-Syn21-GFP-P10
Plasmid#104615PurposeExpresses GFP upon binding of TALE4 to VAS4DepositorInsert3xVAS4
ExpressionInsectPromoterhsp70Available SinceJan. 29, 2018AvailabilityAcademic Institutions and Nonprofits only