We narrowed to 18,529 results for: IRE

1. His6-MBP-uTEV3

   Plasmid

   #135464

   Purpose

   Bacterial expression of uTEV3 (full-length) protease

   Depositor

   Alice Ting

   Article

   Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

   Insert

   His6-MBP-uTEV3
   

   Use

   Tags

   MBP, His6

   Expression

   Bacterial

   Mutation

   S219V mutation (improves stability) , I138T/S153N…

   Promoter

   T5

   Available since

   Jan. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pXR003: CasRx gRNA cloning backbone

   Plasmid

   #109053

   Purpose

   hU6-driven expression of guide RNAs compatible with CasRx. 5' processed DR followed by BbsI sites for guide cloning.

   Depositor

   Patrick Hsu

   Article

   Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSicoR human Dicer1

   Plasmid

   #14763

   Depositor

   Tyler Jacks

   Article

   Kumar et al Nat Genet. 2007 Apr 1. ():.

   Insert

   Dicer shRNA (DICER1 Human)
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 5, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. piggyBAC-H2B mCherry-XL (R125K)

   Plasmid

   #189926

   Purpose

   Expresses nuclear-localized mCherry-XL (R125K) in mammalian cells

   Depositor

   Ralph Jimenez

   Article

   Mukherjee et al J Phys Chem B. 2022 Jun 16. doi: 10.1021

   Insert

   mCherry-XL (R125K)
   

   Use

   Tags

   H2B

   Expression

   Mammalian

   Mutation

   differs from mCherry by R125K, W143S, I161V, Q163…

   Promoter

   CMV7

   Available since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-basP:fLuc-TK:rLuc

   Plasmid

   #138369

   Purpose

   Lentiviral plasmid contains basal promoter driving firefly Luciferase 2 and Renilla Luciferase driven by TK promoter

   Depositor

   Nathan Lawson

   Article

   Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

   Insert

   sequence multi cloning sites, minP driving firefly luciferase and TK promoter driving renilla luciferase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSicoR human Drosha1

   Plasmid

   #14766

   Depositor

   Tyler Jacks

   Article

   Kumar et al Nat Genet. 2007 Apr 1. ():.

   Insert

   Drosha shRNA (DROSHA Human)
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 5, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pTECH-MbAcK3RS(IPYE)

   Plasmid

   #104070

   Purpose

   Expression plasmid encoding MbAcK3RS(IPYE) and pylT

   Depositor

   David Liu

   Article

   Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

   Insert

   Plpp MbAcK3RS(IPYE); PproK pyl
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Contains activating mutations V31I, T56P, H62Y, A…

   Promoter

   Plpp and PproK

   Available since

   Dec. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNP568

   Plasmid

   #222725

   Purpose

   pNP555 - IS621 RE-LE TBL-WT, DBL-WT bRNA in cis

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

   Insert

   IS621 bridge RNA
   

   Use

   Tags

   sfGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. CaMKIIP-mEGFP-P2A-paAIP2/pAAV

   Plasmid

   #91718

   Purpose

   AAV-mediated expression of mEGFP and paAIP2 for optogenetic control of CaMKII

   Depositor

   Ryohei Yasuda

   Article

   Murakoshi et al Neuron. 2017 Apr 5;94(1):37-47.e5. doi:

   Insert

   mEGFP-P2A-paAIP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CaMKII 1.3

   Available since

   June 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pICH47742:: pRPS5a-PE2max-NC::tHSP

    Plasmid

    #227694

    Purpose

    Golden Gate level 1, position 2, PE2max-NC expression cassette for Arabidopsis.

    Depositor

    Jae-Yean Kim

    Article

    Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

    Insert

    PE2max-NC being driven by pRPS5a and terminated by tHSP
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    pRPS5a

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pU6-PspCas13b-gRNA-Actb1216

    Plasmid

    #155368

    Purpose

    PspCas13b guide RNA positioned 8bp 5' of Actb 1216 for targeted m6A RNA methylation

    Depositor

    David Liu

    Article

    Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

    Insert

    PspCas13b gRNA targeting Actb 1216
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNP1830

    Plasmid

    #222728

    Purpose

    pNP561 - T5 Target with T7 Inducible IS621 Recombinase

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

    Insert

    IS621 Recombinase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNP1047

    Plasmid

    #222726

    Purpose

    pNP555 - IS621 RE-LE TBL-T5, DBL-WT bRNA in cis

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

    Insert

    IS621 bridge RNA
    

    Use

    Tags

    sfGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti_EF1a_EV

    Plasmid

    #183116

    Purpose

    Empty vector control for pLenti_EF1a constructs

    Depositor

    William Kaelin

    Article

    Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

    Insert

    Small multiple cloning site
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. Halo-TNFa-RUSH

    Plasmid

    #166901

    Purpose

    RUSH cargo: Str-KDEL_IRES_Tumor Necrosis Factor alpha (TNFa)-SBP-Halo

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

    Insert

    HaloTag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. p2T-CMV-evoCjCas9-PEmax_dRnH-BlastR

    Plasmid

    #194063

    Purpose

    Mammalian expression of evoCjCas9 PEmax_deltaRnH

    Depositor

    Gerald Schwank

    Article

    Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

    Insert

    evoCjCas9-PEmax_dRnH
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJH645

    Plasmid

    #172169

    Purpose

    Reporter plasmid for pylBCD variant in pBAD backbone

    Depositor

    Matthew Bennett

    Article

    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

    Insert

    Pem7 luxCDA(B.1TAG)E; Plpp pylS; PproK pylT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Inserted amber codon immediately after initiator …

    Promoter

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLentiGuide_GFP_NT135

    Plasmid

    #183118

    Purpose

    pLentiGuide modified to express GFP and a non-targeting sgRNA

    Depositor

    William Kaelin

    Article

    Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

    Insert

    NT135
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. CMV-p150

    Plasmid

    #51412

    Purpose

    expression of full length of chicken DCTN1 p150Glued in mammalian cells

    Depositor

    Trina Schroer

    Article

    Quintyne et al J Cell Biol. 1999 Oct 18;147(2):321-34.

    Insert

    DCTN1 (DCTN1 Chicken)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pLentiGuide_mCherry_NT135

    Plasmid

    #183121

    Purpose

    pLentiGuide modified to express mCherry and a non-targeting sgRNA

    Depositor

    William Kaelin

    Article

    Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

    Insert

    NT135
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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