We narrowed to 7,206 results for: RAP

1. pTRE-BI-aaAFB2

   Plasmid

   #207839

   Purpose

   Inducible AFB2 Plasmids for rapid depletion of GFP-tagged proteins in mammalian cells

   Depositor

   Michaela Mueller-McNicoll

   Article

   Arnold et al J Cell Biol. 2024 Feb 5;223(2):e20230403

   Inserts

   atAFB2
   mCherry
   miniIAA7
   VHH-GFP4
   

   Use

   Tags

   3X FLAG-Tag and weak NLS

   Expression

   Mammalian

   Mutation

   All K mutated to R

   Promoter

   TRE3G BI promoter

   Available since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. Epac-S-H90

   Plasmid

   #170340

   Purpose

   CFP(UST)_EPACdDEPCD_cp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

   Depositor

   Kees Jalink

   Article

   van der Krogt et al PLoS ONE. 2008 . 3(4):e1916.

   Insert

   CFP(UST)_EPACdDEPCD_cp173Venus(ST) (RAPGEF3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Deletion of DEP domain, Catalytically dead T781A …

   Promoter

   CMV

   Available since

   June 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti6/V5-DEST-ANLN

   Plasmid

   #31203

   Depositor

   Lynda Chin

   Article

   Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

   Insert

   ANLN (ANLN Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 29, 2011

   Availability

   Academic Institutions and Nonprofits only

4. pLD003-pCMV-APOBEC-Cas9(D10A)-rPB(8kD)

   Plasmid

   #165445

   Purpose

   Expresses ACX, rPB(8kD) in mammalian cells

   Depositor

   Wei Leong Chew

   Article

   Chen et al Nat Commun. 2021 Mar 2;12(1):1384. doi:

   Insert

   APOBEC-nCas9-rPB(8kD)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-mCherry-FKBP-GAP(ARHGAP29)

   Plasmid

   #222632

   Purpose

   Rho inactivation using rapamycin

   Depositor

   Arnold Hayer

   Article

   Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

   Insert

   mCherry-FKBP-GAP(ARHGAP29) (ARHGAP29 Human)
   

   Use

   Tags

   mCherry-FKBP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. TFORF0107

   Plasmid

   #143010

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   TRERF1 (TRERF1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTRF.1 udsVenus (P_JUND)

   Plasmid

   #58692

   Purpose

   A rapidly responsive reporter of endogenous JUND promoter activity (Lentiviral expression vector for reporter ultradestabilized Venus)

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

   Insert

   endogenous promoter of JUND (JUND Human)
   

   Use

   Lentiviral

   Tags

   udsVenus

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA5-eYFP-FKBP-MAD1-AA

   Plasmid

   #114038

   Purpose

   Chemical induction of MAD1 localisation to a specific site in the cell (by rapamycin & FRB)

   Depositor

   Geert Kops

   Article

   Kuijt et al Chromosoma. 2014 Oct;123(5):471-80. doi:

   Insert

   Human MAD1 (MAD1L1 Human)
   

   Use

   Tags

   eYFP-FKBP12

   Expression

   Mammalian

   Mutation

   MAD2 binding deficient MAD1 K541A/L543A

   Promoter

   CMV

   Available since

   Oct. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX459-HypaCas9-mR2-ANLN_sgRNA

   Plasmid

   #183876

   Purpose

   pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and ANLN sgRNA for N-terminal tagging of anillin in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   ANLN sgRNA spacer (ANLN Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. LentiGuide-Puro-NCOA7g1 (BB22)

    Plasmid

    #139457

    Purpose

    Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable marker

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    NCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR (NCOA7 Synthetic)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a / U6

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. LentiGuide-Puro-NCOA7g2 (BB23)

    Plasmid

    #139458

    Purpose

    Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable marker

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    NCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR (NCOA7 Synthetic)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a / U6

    Available since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. TFORF2115

    Plasmid

    #144384

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NCOA6 (NCOA6 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-X1-Neo-GFP-ATL1-G20TM

    Plasmid

    #109019

    Purpose

    stable lentiviral expression of cDNA

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    ATL1-deltaTM (ATL1 Human)
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    truncated form without C-terminal ER transmembran…

    Promoter

    EIF-1a promoter

    Available since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCold I-Hero13

    Plasmid

    #187929

    Purpose

    Bacterial expression of heat-resistant obscure (Hero) protein Hero13

    Depositor

    Yukihide Tomari

    Article

    Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

    Insert

    Hero13 (BEX3 Human)
    

    Use

    Tags

    Flag-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. FKBP-DCRD-YFP

    Plasmid

    #87450

    Purpose

    rapamycin-mediated heterodimerization between PCRD and DCRD from C-terminal of L-type Ca2+ channels

    Depositor

    Xiaodong Liu

    Article

    Liu et al Elife. 2017 Jan 6;6. pii: e21989. doi: 1

    Insert

    DCRD (distal C-terminal regulatory domain) (CACNA1F Human)
    

    Use

    Tags

    6xHis-T7, FKBP, and YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. Epac-S-H96

    Plasmid

    #170342

    Purpose

    CFP(UST)Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

    Depositor

    Kees Jalink

    Article

    van der Krogt et al PLoS ONE. 2008 . 3(4):e1916.

    Insert

    CFP(UST)Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) (RAPGEF3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deletion of DEP domain, Catalytically dead T781A …

    Promoter

    CMV

    Available since

    Oct. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CFP-PCRD-FRB

    Plasmid

    #87449

    Purpose

    rapamycin-mediated heterodimerization between PCRD and DCRD from C-terminal of L-type Ca2+ channels

    Depositor

    Xiaodong Liu

    Article

    Liu et al Elife. 2017 Jan 6;6. pii: e21989. doi: 1

    Insert

    PCRD (proximal C-terminal regulatory domain) (CACNA1F Human)
    

    Use

    Tags

    6xHis-T7, CFP, and FRB

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p-super-retro puro RhoGDI2 shRNA

    Plasmid

    #58897

    Purpose

    produces an shRNA against RhoGDI2

    Depositor

    Channing Der

    Article

    Stevens et al Small GTPases. 2011 Jul;2(4):202-210. Ep

    Insert

    RhoGDI2 (ARHGDIB Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 23, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pLenti6/V5-DEST-ACP5

    Plasmid

    #31204

    Depositor

    Lynda Chin

    Article

    Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

    Insert

    ACP5 (ACP5 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 29, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pmCherry-AHPH

    Plasmid

    #187278

    Purpose

    Express mCherry-AHPH (RhoA biosensor)

    Depositor

    Alpha Yap

    Article

    Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

    Insert

    Anillin and Pleckstrin homology domain (ANLN Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    AHPH

    Promoter

    CMV

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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