Addgene

We narrowed to 21,474 results for: ESC;

Showing: 10651 - 10680 of 21474 results

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dCas9-MSK1

Plasmid

#165601

Purpose

Expresses Sp dCas9 fused to human MSK1

Depositor

Isaac Hilton

Article

Li et al Nat Commun. 2021 Feb 9;12(1):896. doi: 1

Insert

ribosomal protein S6 kinase A5 (RPS6KA5 Synthetic, S. Pyogenes, Human)

Use

CRISPR and Lentiviral

Tags

FLAG Tag

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyso-InPAkt

Plasmid

#184053

Purpose

Lysosome-targeted FRET-based biosensor for monitoring 3-phosphoinositide dynamics in living cells.

Depositor

Jin Zhang

Article

Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

Insert

Lyso-InPAkt

Use

Tags

Lysosome-associated membrane protein 1 (LAMP1).

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-HA-eLACCO2.1-NGR

Plasmid

#208023

Purpose

Biosensor for extracellular L-lactate

Depositor

Robert Campbell

Article

Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

Insert

eLACCO2.1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-MAPTau-N-10

Plasmid

#55312

Purpose

Localization: Microtubules, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

MAPTau (MAPT Human)

Use

Tags

mTagBFP2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pRDB_182

Plasmid

#216103

Purpose

CRISPRi, EF1a-driven dCas9-KRAB (ZIM3) (Cas only)

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

Cas9 [Sp]

Use

CRISPR and Lentiviral; Assembled vector

Tags

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-EGFP-P2A-FusionRed

Plasmid

#191108

Purpose

Mammalian cell line expression plasmids for production of EGFP and FusionRed proteins

Depositor

Kiryl Piatkevich

Article

Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

Insert

pAAV-CAG-EGFP-P2A-FusionRed

Use

Tags

FusionRed

Expression

Mammalian

Mutation

See depositor comments below

Promoter

chicken β-actin promoter

Available since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-Flpo

Plasmid

#183535

Purpose

INTRSECT

Depositor

Karl Deisseroth

Article

Fan et al Cell. 2023 Feb 2;186(3):543-559.e19. doi

Insert

Flpo

Use

AAV

Tags

Expression

Mutation

None

Promoter

CaMKIIa

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pTRIP-CMV-tagRFP-FLAG-cGAS

Plasmid

#86676

Purpose

Expresses catalytically-active tagRFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.

Depositor

Nicolas Manel

Article

Raab et al Science. 2016 Apr 15;352(6283):359-62. d

Insert

tagRFP-FLAG-cGAS

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 18, 2017

Availability

Academic Institutions and Nonprofits only

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pRDB_181

Plasmid

#216102

Purpose

CRISPRi, EF1a-driven dCas9-KRAB (ZNF10) (Cas only)

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

Cas9 [Sp]

Use

CRISPR and Lentiviral; Assembled vector

Tags

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-GRAB_rDA1h

Plasmid

#140557

Purpose

Expresses the red fluorescent high-affinity DA sensor GRAB_rDA1h in neurons

Depositor

Yulong Li

Article

Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

Has Service

AAV9

Insert

Red fluorescent DA sensor GRAB_rDA1h (high affinity)

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

July 29, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-DIO-Ef1a-TVA-2A-N2cG

Plasmid

#172360

Purpose

Targeting envA-pseodotyped RVdG vectors for specific retrograde labeling specifically from cre-expressing neurons

Depositor

Yoav Ben-Simon, Peter Jonas

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

TVA + B19-N2cG

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

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px458_2A_GFP_sgRNA_PELO

Plasmid

#127124

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

gRNA PELO (PELO Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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Cas9-sgRNA-A

Plasmid

#149369

Purpose

Expresses SpCas9 and sgRNA targeting the lenti-CDDR reporter

Depositor

Tarek Abbas

Article

Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592

Inserts

Cas9
sgRNA-A
Puromycin resistance

Use

CRISPR

Tags

3xFLAG, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Mutation

Promoter

CBh, PGK, and U6

Available since

Nov. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pRDA_662

Plasmid

#216101

Purpose

CRISPRi, EFS-driven dCas9-KRAB (ZNF10) (Cas only)

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

Cas9 [Sp]

Use

CRISPR and Lentiviral; Assembled vector

Tags

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pRDA_831

Plasmid

#216100

Purpose

CRISPRa, EF1a-driven dCas9-VP64 (Cas only)

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

Cas9 [Sp]

Use

CRISPR and Lentiviral; Assembled vector

Tags

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCA528 AMSH 1-178

Plasmid

#160050

Purpose

Expresses MIT domain (residues 1-178) of AMSH as a HIS-SUMO fusion protein. Internal ID: WISP18-22.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

AMSH residues 1-178 (STAMBP Human)

Use

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 1-178

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003 DLAT-1

Plasmid

#184484

Purpose

Lentivirus gRNA targeting human DLAT gene

Depositor

Peter Tsvetkov, Todd Golub

Article

Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

Insert

DLAT-1

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003 DLAT-2

Plasmid

#184485

Purpose

Lentivirus gRNA targeting human DLAT gene

Depositor

Peter Tsvetkov, Todd Golub

Article

Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

Insert

DLAT-2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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AnkyrinG-190-GFP

Plasmid

#31059

Depositor

Vann Bennett

Article

Mohler et al J Biol Chem. 2002 Mar 22. 277(12):10599-

Insert

Ankyrin G (Ank3 Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV_CaMKIIa_SERT_EYFP

Plasmid

#198737

Purpose

Serotonin transporter with EYFP fluorescent protein driven by CaMKIIa promoter on a AAV vector

Depositor

Lin Tian

Article

Vargas et al Science. 2023 Feb 17;379(6633):700-706.

Insert

Serotonin transporter and yellow fluorescent protein

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CaMKIIa

Available since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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Hsp70-mplum

Plasmid

#45016

Depositor

Anna Planas

Article

de la Rosa et al Eur J Nucl Med Mol Imaging. 2013 Feb;40(

Insert

mPlum

Use

Tags

Expression

Mammalian

Mutation

Promoter

mouse Hspa1b

Available since

May 24, 2013

Availability

Academic Institutions and Nonprofits only

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pET15b CnA CnB

Plasmid

#11787

Depositor

Anjana Rao

Article

Mondragon et al Biochemistry. 1997 Apr 22. 36(16):4934-4

Insert

CnA CnB (PPP3R1, PPP3CA Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 4, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA-R-iLACCO1

Plasmid

#208025

Purpose

Biosensor for intracellular L-lactate

Depositor

Robert Campbell

Article

Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

Insert

R-iLACCO1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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PIK3CA - Exon20 H1047R

Plasmid

#16639

Depositor

Bert Vogelstein

Article

Samuels et al Science. 2004 Apr 23. 304(5670):554.

Insert

PIK3CA H1047R (PIK3CA Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

H1047R

Promoter

Available since

March 10, 2008

Availability

Academic Institutions and Nonprofits only

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pLENTI_RPS2_Keima

Plasmid

#127139

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS2_Keima (RPS2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-mCherry-anti-mouse-IgG(kappa)-nanobody

Plasmid

#138130

Purpose

Expresses GST-tagged mCherry-anti-mouse-IgG(kappa)-nanobody in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

Insert

mCherry-anti-mouse-IgG(kappa)-nanobody

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.Flex.GCaMP6f.WPRE.SV40

Plasmid

#100835

Purpose

AAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from CAG promoter

Depositor

Douglas Kim, GENIE Project

Article

Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

Has Service

AAV1, AAV5, and AAV9

Insert

GCaMP6f

Use

AAV

Tags

Expression

Mammalian

Mutation

GCaMP3-T302L R303P A317E D380Y T381R S383T R392G

Promoter

CAG

Available since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pLNCX2-mEGFP-CHMP2B

Plasmid

#115329

Purpose

expresses mEGFP-CHMP2B in mammalian cells

Depositor

Sanford Simon

Article

Johnson et al Elife. 2018 Jul 4;7. pii: 36221. doi: 10

Insert

CHMP2B (CHMP2B Human)

Use

Retroviral

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

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mCerulean3-TOMM20-N-10

Plasmid

#55449

Purpose

Localization: Outer Mitochondrial Membrane, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

TOMM20 (TOMM20 Human)

Use

Tags

mCerulean3

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only
pDule2-Acd82

Plasmid

#174100

Purpose

Acridone82 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.

Depositor

Ryan Mehl

Article

Jones et al Chem Sci. 2021 Aug 3;12(36):11955-11964.

Insert

Acridone 82 tRNA synthetase and tRNA

Use

Tags

Expression

Bacterial

Mutation

N311S, C313G, V366A, W382T

Promoter

Available since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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