We narrowed to 15,838 results for: grna

1. pDGO186N-KS3

   Plasmid

   #174303

   Purpose

   Replicating plasmid with sgRNA cassette containing a killing spacer #3 targeting the wild type polC gene.

   Depositor

   Lee Lynd

   Article

   Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

   Insert

   spacer expression cassette
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)_g1_CASH-1

   Plasmid

   #188975

   Purpose

   A human codon-optimized SpCas9 nickase and chimeric g1 guide RNA expression plasmid.

   Depositor

   Sangdun Choi

   Article

   Javaid et al Stem Cells Int. 2022 Sep 22;2022:4537335

   Insert

   g1 guide RNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)_g2_CASH-1

   Plasmid

   #188976

   Purpose

   A human codon-optimized SpCas9 nickase and chimeric g2 guide RNA expression plasmid.

   Depositor

   Sangdun Choi

   Article

   Javaid et al Stem Cells Int. 2022 Sep 22;2022:4537335

   Insert

   g2 guide RNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMpGE010-Target2 (Mpphot)

   Plasmid

   #186729

   Purpose

   Binary vector for CRISPR/Cas9 (target 2: Mpphot [negative control]) in plants (for Agrobacterium-mediated genetic transformation).

   Depositor

   Yutaka Kodama

   Article

   Konno et al Biochem Biophys Res Commun. 2018 Sep 3;5

   Insert

   Mphot
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. shRNA-Ratio-PosCon

   Plasmid

   #183553

   Purpose

   Designed to test the efficacy of shRNA by assessing the ratio of GFP-fusion protein to RFP (shRNA ratio positive control (Pbrm1), Figure S2)

   Depositor

   Erik Dent

   Article

   Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

   Insert

   Pbrm1 shRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available Since

   June 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. shRNA-Ratio-NegCon

   Plasmid

   #183554

   Purpose

   Designed to test the efficacy of shRNA by assessing the ratio of GFP-fusion protein to RFP (shRNA ratio negative control (Luciferase), Fig S2)

   Depositor

   Erik Dent

   Article

   Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

   Insert

   Luciferase shRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available Since

   June 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTAG4E

   Plasmid

   #176868

   Purpose

   First sgRNA in a tRNA separated array of sgRNAs

   Depositor

   Daniel Voytas

   Article

   Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

   Insert

   sgRNA_4E
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available Since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTAG5

   Plasmid

   #176869

   Purpose

   First sgRNA in a tRNA separated array of sgRNAs

   Depositor

   Daniel Voytas

   Article

   Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

   Insert

   sgRNA_5
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available Since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTAG6E

   Plasmid

   #176870

   Purpose

   First sgRNA in a tRNA separated array of sgRNAs

   Depositor

   Daniel Voytas

   Article

   Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

   Insert

   sgRNA_6E
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available Since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTAG2

    Plasmid

    #176864

    Purpose

    First sgRNA in a tRNA separated array of sgRNAs

    Depositor

    Daniel Voytas

    Article

    Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

    Insert

    sgRNA_2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available Since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTAG3

    Plasmid

    #176866

    Purpose

    First sgRNA in a tRNA separated array of sgRNAs

    Depositor

    Daniel Voytas

    Article

    Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

    Insert

    sgRNA_3
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available Since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTAG4

    Plasmid

    #176867

    Purpose

    First sgRNA in a tRNA separated array of sgRNAs

    Depositor

    Daniel Voytas

    Article

    Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

    Insert

    sgRNA_4
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available Since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLeGO.sgGata1.4.RUNX1A.iG2

    Plasmid

    #181976

    Purpose

    gene knock out of mouse GATA1, overexpression of 3xFLAG-RUNX1A (human)

    Depositor

    Jan-Henning Klusmann

    Inserts

    GATA1 gRNA: CCTAGACCAGGAAAATCCAT (Gata1 Mouse)
    RUNX1A (RUNX1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available Since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pX459-sg-CMV152

    Plasmid

    #179916

    Purpose

    All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting cytomegaloviral promoter site 152.

    Depositor

    Indeever Madireddy, Johan Sosa

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    CMV promoter sgRNA
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pX459-sg-CMV51

    Plasmid

    #179915

    Purpose

    All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting cytomegaloviral promoter site 51.

    Depositor

    Indeever Madireddy, Johan Sosa

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    CMV promoter sgRNA
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pX459-sg-CMV162

    Plasmid

    #179917

    Purpose

    All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting cytomegaloviral promoter site 162.

    Depositor

    Indeever Madireddy, Johan Sosa

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    CMV promoter sgRNA
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pX459-sg-mGreenLantern332

    Plasmid

    #179914

    Purpose

    All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting mGreenLantern fluorescent protein site 332.

    Depositor

    Indeever Madireddy, Johan Sosa

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    mGreenLantern sgRNA
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PX552-rScn2a-3xV5-Syn-miRFP670

    Plasmid

    #182566

    Purpose

    For rat Nav1.2 knockin with 3xV5 at the extracellular loop

    Depositor

    James Zhe Liu

    Article

    Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

    Inserts

    rat Scn2a gRNA and 3xV5 donor DNA
    miRFP670
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Syn and U6

    Available Since

    March 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMCL8

    Plasmid

    #176184

    Purpose

    gRNA cloning cassette with RPR1p-tetO promoter and terminator RPT1t, RPR1p-tetO-GFP-scRNA-RPR1t

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMCL8_28bp

    Plasmid

    #176185

    Purpose

    plasmid containing scaffold gRNA for Cas9 followed by a 28bp Csy4 recognition sequence, RPR1p-tetO-GFP-scRNA-28bpCsy4-RPR1t

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMCL9

    Plasmid

    #176186

    Purpose

    2 mu cloning vector for single or multiplexed gRNAs, SNR52p-sfGFP-scRNA-SUP4t-CYC1t

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pU6-TREX1 g3 Cas9-T2A-mCherry

    Plasmid

    #164252

    Purpose

    Transcription of TREX1 guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

    Depositor

    John Maciejowski

    Article

    Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

    Insert

    sgTREX1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pPPC008.106

    Plasmid

    #171141

    Purpose

    Expression of J106 scRNA on pBBR1-GmR plasmid

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

    Insert

    J106 scRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119

    Available Since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pJJF144_SECGFP_sg2

    Plasmid

    #163865

    Purpose

    sgRNA targeting GFP in C. elegans. Backbone: pJJR50.

    Depositor

    Nikolaus Rajewsky

    Article

    Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

    Insert

    sgRNA targeting GFP
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    R07E5.16 (U6)

    Available Since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJJF143_SECGFP_sg1

    Plasmid

    #163864

    Purpose

    sgRNA targeting GFP in C. elegans. Backbone: pJJR50.

    Depositor

    Nikolaus Rajewsky

    Article

    Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

    Insert

    sgRNA targeting GFP
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    R07E5.16 (U6)

    Available Since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. sgiRFP1

    Plasmid

    #113972

    Purpose

    Single short guide RNA targeting GATCGAGTTCGAGCCTGCGG in iRFP670 sequence

    Depositor

    Jordan Green

    Article

    Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

    Insert

    iRFP670 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. sgiRFP2

    Plasmid

    #113973

    Purpose

    Single short guide RNA targeting GCGCGTTCTTTGGACGCGA in iRFP670 sequence

    Depositor

    Jordan Green

    Article

    Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

    Insert

    iRFP670 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. sgiRFP3

    Plasmid

    #113974

    Purpose

    Single short guide RNA targeting CGTGATGTTGTACCGCTTC in iRFP670 sequence

    Depositor

    Jordan Green

    Article

    Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

    Insert

    iRFP670 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. RIB VP64 R2

    Plasmid

    #161494

    Purpose

    CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_RIB1_R2_gRNA_MS2

    Depositor

    Diethard Mattanovich, Brigitte Gasser

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2-VP64
    gRNA (Targeting R2 in the RIB1 promoter)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pGAP, pPOR1, and pTEF2

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. RIB VP64 R3

    Plasmid

    #161495

    Purpose

    CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_RIB1_R3_gRNA_MS2

    Depositor

    Diethard Mattanovich, Brigitte Gasser

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2-VP64
    gRNA (Targeting R3 in the RIB1 promoter)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pGAP, pPOR1, and pTEF2

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge