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pGV15 (CK2alpha K68M)

Plasmid

#27089

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Insert

CK2alpha K68M (kinase dead) (CSNK2A1 Human)

Tags

HA

Expression

Mammalian

Mutation

K68M (kinase dead)

Promoter

CMV

Available Since

May 17, 2012

Availability

Academic Institutions and Nonprofits only
pWPXL-Sox4

Plasmid

#36984

Depositor

Bob Weinberg

Article

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

Sox4 (SOX4 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 13, 2012

Availability

Academic Institutions and Nonprofits only

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PB-SRT-tdTomato

Plasmid

#154885

Purpose

piggyBac self-reporting transposon with tdTomato marker for mammalian calling cards

Depositor

Joseph Dougherty, Rob Mitra

Article

Moudgil et al Cell. 2020 Aug 20;182(4):992-1008.e21. d

Insert

piggyBac SRT

Use

Transposon

Expression

Mammalian

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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FLAG-Foxo1-WT 6KR

Plasmid

#17560

Depositor

Domenico Accili

Article

Kitamura et al Cell Metab. 2005 Sep . 2(3):153-63.

Insert

Foxo1 6KR (Foxo1 Mouse)

Tags

Flag and Myc

Expression

Mammalian

Mutation

K242, K245, K259, K262, K271 and K291 were replac…

Promoter

CMV

Available Since

Oct. 20, 2008

Availability

Academic Institutions and Nonprofits only
pLKO human shRNA 2 DEPTOR

Plasmid

#21336

Depositor

David Sabatini

Article

Peterson et al Cell. 2009 May 29;137(5):873-86. doi: 10

Insert

DEPTOR shRNA 2 (DEPTOR Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only
FASTHDR-Cterm-mRuby3-Zeo

Plasmid

#167206

Purpose

Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mRuby3 and selection with Zeocin

Depositor

Oscar Perez

Article

Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

Type

Empty backbone

Use

CRISPR

Tags

mRuby3

Expression

Mammalian

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pSJ1726 (pFA6-NATMX-CDC42pr-KAR2SS-GFP1-10)

Plasmid

#86421

Purpose

N-terminal PCR tagging cassette for yeast codon optimized split GFP1-10 with CDC42 promoter and KAR2 signal sequence

Depositor

Sue Jaspersen

Article

Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

Insert

CDC42pr-KAR2SS-GFP1-10

Tags

GFP1-10

Expression

Yeast

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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TRE-UBC-mCD24-T2A-GFP

Plasmid

#205448

Purpose

lentiviral plasmid for expression of mouse CD24

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

mCD24 (Cd24a Mouse)

Expression

Mammalian

Promoter

UBC

Available Since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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PB-SRT-Puro

Plasmid

#154884

Purpose

piggyBac self-reporting transposon with puromycin marker for mammalian calling cards

Depositor

Joseph Dougherty, Rob Mitra

Article

Moudgil et al Cell. 2020 Aug 20;182(4):992-1008.e21. d

Insert

piggyBac SRT

Use

Transposon

Expression

Mammalian

Available Since

July 29, 2020

Availability

Academic Institutions and Nonprofits only

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pET28-His-MBP-NLS-RfxCas13d-NLS-HA

Plasmid

#171586

Purpose

Bacterial expression of NLS-RfxCas13d-NLS-HA

Depositor

Neville Sanjana

Article

Mendez-Mancilla et al Cell Chem Biol. 2022 Feb 17;29(2):321-32

Insert

His-MBP-NLS-RfxCas13d-NLS-HA

Tags

HA

Expression

Bacterial

Available Since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pBR_attB(bxb)_ccdB_lox

Plasmid

#183763

Purpose

Bxb1-specific donor plasmid for the cloning of multiple inserts by Golden Gate assembly (BpiI)

Depositor

Richard Davis

Article

Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

Insert

ccdB

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-Pickles 2.34NES

Plasmid

#100028

Purpose

Improved FRET biosensor for the measurement of BCR-ABL activity.

Depositor

Yusuke Ohba

Article

Horiguchi et al Cell Struct Funct. 2017 Feb 2;42(1):15-2

Insert

Pickles-2.34NES

Use

Pfx vector is derived from pcmv vector.

Expression

Mammalian

Promoter

CMV

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pLX317 PRDM14

Plasmid

#193682

Purpose

Constitutive lentiviral expression of PRDM14

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

PRDM14 (PRDM14 Human)

Use

Lentiviral

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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vDip-C: pAAV-Pcp2-mGreenLantern-KASH

Plasmid

#207613

Purpose

vDip-C AAV vector with mouse Pcp2 (also known as L7) promoter driving green fluorophore mGreenLantern with a KASH nuclear membrane tag to isolate cell nuclei of cerebellar Purkinje cells

Depositor

Longzhi Tan

Article

Tan et al Science. 2023 Sep 8;381(6662):1112-1119.

Insert

mGreenLantern

Use

AAV

Tags

KASH

Promoter

mouse Pcp2 promoter (L7-6)

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pAS_4xMma PylT_FLAG-AcKRS

Plasmid

#140021

Purpose

Plasmid with 4xMmaPylT cassette and MmaPylRS for amber suppression and incorporation of acetyl-lysine; for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

AcKRS-3 (pylS Methanosarcina mazei)

Tags

FLAG

Expression

Mammalian

Mutation

L301M, L305I, Y306F, L309A, C348F

Promoter

EF1

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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3XHalo_GFP_TRF1#1

Plasmid

#164644

Purpose

tag TRF1 with GFP and Halo

Depositor

Michael Lampson, Huaiying Zhang

Article

Zhang et al Mol Biol Cell. 2020 Aug 15;31(18):2048-2

Insert

TRF1 (TERF1 Human)

Use

Cre/Lox

Expression

Mammalian

Promoter

CAG

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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p21 shRNA1

Plasmid

#25868

Depositor

Sally Temple

Article

Fasano et al Cell Stem Cell. 2007 Jun 7. 1(1):87-99.

Insert

p21 (Cdkn1a Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 20, 2010

Availability

Academic Institutions and Nonprofits only

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mKate2-P2A-APEX2-2xFYVE_hrs

Plasmid

#67663

Purpose

Mammalian expression vector driving APEX2-2xFYVE (2xMouse FYVE domain from HRS, [marks PI3P] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

mKate2-P2A-APEX2-2xFYVE

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pRetroX-PTuner DD-M.EcoGII-v5-Telomeric repeat-binding-factor1

Plasmid

#122084

Purpose

Express M.EcoGII fused to TRF1 with detabilization domain - inducible

Depositor

Laure Crabbe

Article

Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5

Insert

DD-linker-M.EcoGII-V5-TERF1

Use

Retroviral

Tags

V5 tag on Nter of TERF1

Expression

Bacterial and Mammalian

Available Since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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pTY-EF1a-wild type mKdm2b-Flag

Plasmid

#61739

Purpose

Express wild-type mouse histone demethylase Kdm2b

Depositor

Yi Zhang

Article

He et al Nat Cell Biol. 2013 Apr;15(4):373-84. do

Insert

Kdm2b (Kdm2b Mouse)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 6, 2015

Availability

Academic Institutions and Nonprofits only